A.S.Sadykov Institute of Bioorganic Chemistry

According to the literature, Ájuga turkestánica extract has an effective impact on diseases of the cardiovascular system. Aortic smooth muscle ion transport systems play an important role in the development of these diseases1. This study investigated the effects of Ájuga turkestánica extract on ion transport systems in rat aortic smooth muscle preparations under isometric conditions in vitro, and it was found that this extract exerts a strong dose-dependent relaxant effect on the contraction induced by KCl (50 mM) From the obtained results, the effect of this extract on potential-dependent Ca2+ channels was estimated, and in order to confirm the assumption, it was compared with verapamil, a specific blocker of this channel, and the effect of Ájuga turkestánica extract on potential-dependent Ca2+ channels was determined. In the course of the studies, it was assumed that the investigated extract affects not only the potential-dependent Ca2+ channels, but also the receptor-dependent Ca2+ channels, the Ca2+ transport system in the sarcoplasmic reticulum, and the endothelium-dependent relaxation mechanisms. According to the results of the study, it was found that Ájuga turkestánica extract has an effective effect on these ion transport systems (the results obtained are described in detail in the article below).

ctive: As a result of the study, branched polysaccharides were isolated from the basidiomycete raw materials of Ganoderma lucidum. It has been established that the isolated fractions contain branched polysaccharides in the form of complexes with melanin. Methods: After purification of polysaccharides by ion-exchange chromatography, two fractions were obtained from basidial raw materials: neutral polysaccharides GW-1 with a yield of 25.71% and anionic polysaccharides GW-2, the yield of which was 5.26%, respectively. The physicochemical properties of the obtained samples were studied by IR and UV spectroscopy. The degree of purity of the obtained fractions of branched polysaccharides was established. Using gas chromatography, one-dimensional (13C NMR, 1H NMR) and two-dimensional (COSY, TOCSY, HSQC, HMBC, NOESY) NMR spectroscopy, the composition and molecular structure of the obtained polysaccharide samples were determined. Results and Discussion: The results showed that the isolated and purified polysaccharides are branched glucans with 1,4,6- and 1,3,6-bonds between glucopyranose units. Pharmacotoxicological studies were carried out on white outbred mice and it was found that the resulting polysaccharides belong to class V, practically non-toxic compounds (LD50³ 2000 mg/kg). Conclusions: Isolated polysaccharides (GW) are promising biologically active components, on the basis of which it is possible to create drugs with hepatoprotective and antitumor activity.

Изучена структурно-функциональная зависимость экстенсиноподобных белков (ЭПБ) хлопчатни­ка. Установлено, что ЭПБ тормозят пролиферацию клеток и действие их зависит от дозы и фазы клеточного цикла. Антипролиферативный эффект дегликозилированного ЭПБ на клетки линии КМЛ имеет более выраженный характер, чем самих ЭПБ. В работе использованы культура клеток линии КМЛ и методы молекулярного моделирования.

AbstractThe paper presents the results of comprehensive study of SiC samples, which is one of the most promising materials in the field of semiconductor nanotechnology. The identification and qualitative analysis of a SiC sample was carried out by Raman spectroscopy using an In Via Raman spectrometer. Based on the spectrogram of X-ray phase analysis, the amorphous and crystalline phases of this substance were determined. At the same time, according to the peaks of the spectrogram, based on the Miller indices and inter planar distances dhkl, complex compounds are shown that are newly formed on the basis of silicon carbide. Using the method of IR spectroscopy, the IR spectra of SiC were obtained in the optical range ν = 400-4000 cm−1.Keywords: SiC samplesIR spectroscopyRaman spectroscopyX-ray phase analysisgamma irradiation Disclosure StatementNo potential conflict of interest was reported by the author(s).

Получены 12 стабильных гибридом, продуцирующих моноклональные антитела (мкАТ) к мемб­ранным белкам, выделенным из двухдневных проростков хлопчатника. Твердофазным иммуноферментным методом ELISA изучена перекрестная реактивность мкАТ к мембранным белкам: выявлены антитела к белкам клеточной стенки хлопчатника (семейство экстенсинов) и различным фракциям лектиноподобных белков.

Установлена антипролиферативная активность экстенсино- (ЭПБ) и лектиноподобных (ЛПБ) белков, выделенных из хлопчатника, по включению ³Н-тимидина в клетках суспензионной культуры хлопчатника, Показано зависящее от времени инкубации ингибиторное действие ЭПБ и ЛПБ.

The article is devoted to obtaining water-soluble complexes of iminoazo derivatives of gossypol, studying their physicochemical properties and biological activity. Aromatic, heterocyclic amines and sulfanilamide preparations were used as amine components in obtaining iminoazo derivatives of gossypol and their water-soluble complexes. The maximum values of wavelengths and the corresponding optical densities were determined in the UV spectra of compounds in acetone solvent. In order to determine the structure of the compounds, the infrared spectrum was taken and analyzed: the shift of the fundamental vibrational frequencies up to 31 cm-1 showed that the water-poly-N-vinylpyrrolidone in the compound is connected to a lesser extent as a result of hydrogen bonding. Obtaining complexes of azoderivatives of gossypol imines with poly-N-vinylpyrrolidone is related to the multi-functionality of the reactive groups of the ligand compound; formed hydrogen bonds due to the oxygen of the cycloamide group. For the first time, six new water-soluble complexes iminoazo derivatives of gossypol with poly-N-vinylpyrrolidone were obtained. The results of determination of interferon-inducing activity of compounds were analyzed. The activity was compared with the effectiveness of azo-, iminoazo derivatives of gossypol. According to the obtained results, it was shown that the effectiveness of water-soluble complexes iminoazo derivatives of gossypol.

Nanosized titanium dioxide, being a wide-gap semiconductor, has a high potential for use in electronics and electro-optics due to transparency in the visible region of the spectrum and a high refractive index [1-3]. Various peaks in the X-ray phase analysis (XRD) spectrum of titanium oxide TiO2 samples were experimentally detected. The Miller indices and lattice parameters were determined on the basis of XRD data for titanium oxide TiO2 samples obtained for samples with different powder preparation modes. The obtained experimental data are in good agreement with the data obtained by other methods.

Abstract The alkaloid triacanthine was isolated from Gleditschia triacanthos and its di- and tribenzyl derivatives were synthesized. Both compounds are crystallized in the R-3 space group with same unit cell parameters. A channel is formed in the crystals, in which water molecules with large thermal parameters located on the c axis on H-bonds distances. In the dibenzyl derivative of triacanthine, the prenyl group is disordered in two positions in a ratio of 0.65:0.35, which makes it possible to fill voids in the crystal packing. The Hirshfeld surface analysis indicates that the most important contributions to the crystal packing are from H…H (about 57.9% and 51.4% for di- and tribenzyl derivatives respectively) and H…C/C…H (around 14.9% and 19.2%) interactions.

Potential competing interests: No potential competing

Capparis spino sa L. is a xerophytic species with high resistance to drought, extreme temperatures, and soil moisture deficiency. Due to its biological characteristics, the plant effectively prevents soil erosion and land degradation. This study focuses on the role of mineral metabolism in the adaptation of C. spinosa to saline conditions. Based on a comparative analysis of the elemental composition of soils and vegetative plant organs sampled from contrasting ecotopes, the key mechanisms of salt tolerance in this species have been identified. In soils and C. spinosa samples, 25 elements were identified and quantified: nine macroelements (K, Ca, Na, Mg, Fe, Al, Sr, S, P), twelve microelements, and four heavy metals (Pb, Cd, Cr, Hg). The content of macroelements in soils decreased in the following sequence: Ca, Fe, Al, K, Na, Mg, Sr, P, S. In the studied C. spinosa samples, highly efficient potassium (K) accumulation was detected (BAC > 10), while sulfur (S), phosphorus (P), and sodium (Na) exhibited hyperaccumulation properties (BAC > 1.0–10.0). Magnesium (Mg) and strontium (Sr) showed moderate accumulation (BAC > 0.1–1.0). C. spinosa accumulates calcium (Ca), iron (Fe), and aluminium (Al) in limited quantities to support metabolic processes (BAC > 0.01–0.1).

Abstract Single crystals of 4-amino-3-nitrobenzoic acid polymorphic structure (4-A3NBA-P) suitable for X-ray analysis were obtained by the slow evaporation method. New compound structure were studied by IR and UV spectroscopy, thermogravimetric analysis, elemental analysis, as well as Hirshfeld surface analysis and Molecular Docking. Two independently crystallographically distinct molecules that differ only slightly in their geometrical properties make up the compound's asymmetric unit. Depicts the compound's packing diagram, which is made up of four molecules of 4-A3NBA-P. The previously reported 4-A3NBA-P belongs to the monoclinic crystal system with centric space group P21/c (at 100 K) and one molecule in the asymmetric unit, whereas the crystal packs in the triclinic system with a centrosymmetric space group Pī. The cumulative expanse of the Hirschfeld surface encompasses 362.65 Å 2 . Within this intricate mosaic, a substantive 41.9% is ascribed to interactions of the O•••H/H•••O ilk, while a notable 21.8% is attributable to H•••H interactions. According to molecular docking experiments, the ligand has a strong propensity to bind to KDM4 proteins and the binding energy has improved. The obtained antimicrobial activity data attest that the compounds under study have some antibiotic potential and can be used to make preparations to slow the growth and development of pathogenic bacteria.

The intensive development of agricultural production currently requires the expansion of the use of chemical plant protection products from insect pests, which in turn leads to environmental pollution and irreparable losses in the biocenosis. Therefore, the development of fundamentally new plant protection products, characterized by safety in relation to the environment and high selectivity of action, is becoming more and more urgent. The use of sex pheromones in integrated plant protection systems leads to the need to develop convenient synthesis schemes that allow obtaining pheromones of various pest species with good yield and high isomeric purity from the same starting compounds-synthons.

In this thesis, the amount of carbohydrates in grape molasses is determined and information about the healing properties of grapes is presented.

Геномная «дактилоскопия» с использованием в качестве гибридизаиионной пробы ДНК фага M13 находит все более широкое применение в области исследования структуры генома растений.Сравнительный анализ гипервариабельных участков генома различных сортов и видов хлопчатника Gossypium показал, что эта технология позволяет выявить межсортовые и межвидовые различия.

The article provides information on the preparation and study of the structural and biological properties of water-soluble complexes of gossypol with N-polyvinylpyrrolidone. The most suitable method for obtaining complexes of N-polyvinylpyrrolidone with gossypol derivatives turned out to be a method that represents the co-precipitation of the starting gossypol with a polymer in non-aqueous solvent systems with stirring. In this way, a water-soluble complex of gossypol with N-polyvinylpyrrolidone was obtained. The resulting complex is a brightly colored powdery substance, completely soluble in water. Its physicochemical parameters are characterized. The structure of the resulting complex was confirmed by spectroscopy (UV, IR). In the course of the work, the relaxing effect of the PGN-02 polyphenol on the preparation of rat aorta was studied for the first time. Biological studies have shown that polyphenol PGN-02 has a strong relaxing effect and significantly reduces the force of contraction of the aorta caused by KCl (50 mM). It was shown that as a result of blocking the voltage-dependent L-type Ca2+ channels located in the plasmalemma, muscle relaxation occurred. The relaxing effect of PGN-02 polyphenol is associated with the blocking of L-type Ca2+ channels, as evidenced by the results of experiments conducted with the specific Ca2+ channel blocker verapamil.