Agencia Nacional de Investigación y Desarrollo

protection of health in the near and long term. Several essential steps need to be taken to transform the economy to support planetary health. These steps include a reduction of waste through the creation of products that are more durable and require less energy and materials to manufacture than those often produced at present; the incentivisation of recycling, reuse, and repair; and the substitution of hazardous materials with safer alternatives.

Significance In the current changing climate, it is essential to improve crop production and resilience under dry and nutrient-poor conditions. Desert plants have naturally evolved to flourish under such conditions. Therefore, understanding the underlying mechanisms for their adaptation can potentially help to ensure food security. The Atacama Desert, the driest nonpolar place on Earth, offers a unique opportunity to explore plant adaptations to extreme environmental conditions. Here, we reveal how the adaptive strategies common or specific to the major plant lineages in the Atacama include enrichment of plant growth-promoting bacteria near their roots and positive selection of genes that are associated with key beneficial processes for plant survival. These strategies can potentially direct the molecular breeding or engineering of resilient crops.

Bacterial motility is a widespread characteristic that can provide several advantages for the cell, allowing it to move towards more favorable conditions and enabling host-associated processes such as colonization. There are different bacterial motility types, and their expression is highly regulated by the environmental conditions. Because of this, methods for studying motility under realistic experimental conditions are required. A wide variety of approaches have been developed to study bacterial motility. Here, we present the most common techniques and recent advances and discuss their strengths as well as their limitations. We classify them as macroscopic or microscopic and highlight the advantages of three-dimensional imaging in microscopic approaches. Lastly, we discuss methods suited for studying motility in bacterial-host interactions, including the use of the zebrafish model.

The rise of advanced chatbots, such as ChatGPT, has stirred excitement and curiosity in the scientific community. Powered by large language models (LLMs) based on generative pretrained transformers (GPTs)-specifically GPT-3.5 and GPT-4-ChatGPT is considered a general-purpose technology with the potential to impact the job market and research endeavors in numerous fields Although similar models have been fine-tuned for biology-specific projects, including text-based analysis and biological sequence decoding [2,3], ChatGPT provides a natural interface for bioinformaticians to begin using LLMs in their activities. This tool is already accelerating various activities undertaken by computational biologists, ranging from data cleaning to interpreting results and publishing. However, with great power comes great responsibility. As scientists, we must harness the full potential of ChatGPT while adhering to ethical guidelines and avoiding pitfalls associated with the technology.

The Quaternary Nevado de Longaví volcano of the Andean Southern Volcanic Zone (SVZ) has erupted magmas that range in composition from basalt to low-silica dacite, although andesites are the dominant erupted magma type. Amphibole is a common phenocryst phase in andesites throughout the volcano, and it is the dominant mafic phase in Holocene dacites and their included mafic enclaves. Compositions of magmas erupted at Longaví volcano define arrays that diverge from trends delineated by neighboring frontal-arc volcanoes. Although mafic compositions at Longaví are broadly similar to basalts at other SVZ centers, Longaví intermediate and evolved magmas have systematically lower abundances of incompatible major (K2O, P2O5) and trace elements (Rb, Zr, Nb, REE, Th, etc), as well as high Ba/Th, Sr/Y, and La/Yb ratios. Longaví volcano magmas define two differentiation series with regard to enrichments of Rb (and other incompatible elements) with increasing silica. A high-Rb series that includes the oldest units of the volcano comprises basalts to andesites dominated by anhydrous mineral assemblages with chemical compositions similar to other SVZ magmatic series. The series with low Rb, on the other hand, includes the Holocene units that evolved from basaltic andesites to dacites by means of fractional crystallization wherein amphibole and calcic plagioclase dominate the mineral assemblage. Magmas parental to low-Rb series are interpreted to be high-degree mantle melts, highly hydrous and oxidized, formed as a response to high fluid inputs into the subarc mantle. Enhanced water transport to the subarc mantle is a plausible effect of the subduction of the oceanic Mocha Fracture Zone that projects beneath Nevado de Longaví. Volcanoes located over oceanic fracture zones further south along the SVZ have erupted hornblende-bearing magmas that share some chemical similarities with Longaví volcano magmas

The spreading of mesenchymal-like cell layers is critical for embryo morphogenesis and tissue repair, yet we know little of this process in vivo. Here we take advantage of unique developmental features of the non-conventional annual killifish embryo to study the principles underlying tissue spreading in a simple cellular environment, devoid of patterning signals and major morphogenetic cell movements. Using in vivo experimentation and physical modelling we reveal that the extra-embryonic epithelial enveloping cell layer, thought mainly to provide protection to the embryo, directs cell migration and the spreading of embryonic tissue during early development. This function relies on the ability of embryonic cells to couple their autonomous random motility to non-autonomous signals arising from the expansion of the extra-embryonic epithelium, mediated by cell membrane adhesion and tension. Thus, we present a mechanism of extra-embryonic control of embryo morphogenesis that couples the mechanical properties of adjacent tissues in the early killifish embryo.

root as a function of time. We found that gene-expression response to nitrate is dynamic and highly localized and predicted cell type-specific transcription factor (TF)-target interactions. Among cell types, the endodermis stands out as having the largest and most connected nitrate-regulatory gene network. ABF2 and ABF3 are major hubs for transcriptional responses in the endodermis cell layer. We experimentally validated TF-target interactions for ABF2 and ABF3 by chromatin immunoprecipitation followed by sequencing and a cell-based system to detect TF regulation genome-wide. Validated targets of ABF2 and ABF3 account for more than 50% of the nitrate-responsive transcriptome in the endodermis. Moreover, ABF2 and ABF3 are involved in nitrate-induced lateral root growth. Our approach offers an unprecedented spatiotemporal resolution of the root response to nitrate and identifies important components of cell-specific gene regulatory networks.

Volcanic cones are frequently near their gravitational stability limit, which can lead to lateral collapse of the edifice, causing extensive environmental impact, property damage, and loss of life. Here, we examine lateral collapses in mafic arc volcanoes, which are relatively structurally simple edifices dominated by a narrow compositional range from basalts to basaltic andesites. This still encompasses a broad range of volcano dimensions, but the magma types erupted in these systems represent the most abundant type of volcanism on Earth and rocky planets. Their often high magma output rates can result in rapid construction of gravitationally unstable edifices susceptible both to small landslides but also to much larger-scale catastrophic lateral collapses. Although recent studies of basaltic shield volcanoes provide insights on the largest subaerial lateral collapses on Earth, the occurrence of lateral collapses in mafic arc volcanoes lacks a systematic description, and the features that make such structures susceptible to failure has not been treated in depth. In this review, we address whether distinct characteristics lead to the failure of mafic arc volcanoes, or whether their propensity to collapse is no different to failures in volcanoes dominated by intermediate (i.e., andesitic-dacitic) or silicic (i.e., rhyolitic) compositions? We provide a general overview on the stability of mafic arc edifices, their potential for lateral collapse, and the overall impact of large-scale sector collapse processes on the development of mafic magmatic systems, eruptive style and the surrounding landscape. Both historical accounts and geological evidence provide convincing proofs of recurrent (and even repetitive) large-scale (>0.5 km 3 ) lateral failure of mafic arc volcanoes. The main factors contributing to edifice instability in these volcanoes are: (1) frequent sheet-like intrusions accompanied by intense deformation and seismicity; (2) shallow hydrothermal systems weakening basaltic rocks and reducing their overall strength; (3) large edifices with slopes near the critical angle; (4) distribution along fault systems, especially in transtensional settings, and; (5) susceptibility to other external forces such as climate change. These factors are not exclusive of mafic volcanoes, but probably enhanced by the rapid building of such edifices.

A plant's response to external and internal nitrogen signals/status relies on sensing and signaling mechanisms that operate across spatial and temporal dimensions. From a comprehensive systems biology perspective, this involves integrating nitrogen responses in different cell types and over long distances to ensure organ coordination in real time and yield practical applications. In this prospective review, we focus on novel aspects of nitrogen (N) sensing/signaling uncovered using temporal and spatial systems biology approaches, largely in the model Arabidopsis. The temporal aspects span: transcriptional responses to N-dose mediated by Michaelis-Menten kinetics, the role of the master NLP7 transcription factor as a nitrate sensor, its nitrate-dependent TF nuclear retention, its "hit-and-run" mode of target gene regulation, and temporal transcriptional cascade identified by "network walking." Spatial aspects of N-sensing/signaling have been uncovered in cell type-specific studies in roots and in root-to-shoot communication. We explore new approaches using single-cell sequencing data, trajectory inference, and pseudotime analysis as well as machine learning and artificial intelligence approaches. Finally, unveiling the mechanisms underlying the spatial dynamics of nitrogen sensing/signaling networks across species from model to crop could pave the way for translational studies to improve nitrogen-use efficiency in crops. Such outcomes could potentially reduce the detrimental effects of excessive fertilizer usage on groundwater pollution and greenhouse gas emissions.

Enhancing grain yield is a primary goal in the cultivation of major staple crops, including wheat. Recent research has focused on identifying the physiological and molecular factors that influence grain weight, a critical determinant of crop yield. However, a bottleneck has arisen due to the trade-off between grain weight and grain number, whose underlying causes remain elusive. In a novel approach, a wheat expansin gene, TaExpA6 , known for its expression in root tissues, was engineered to express in the grains of the spring wheat cultivar Fielder. This modification led to increases in both grain weight and yield without adversely affecting grain number. Conversely, a triple mutant line targeting the gene TaGW2 , a known negative regulator of grain weight, resulted in increased grain weight but decreased grain number, potentially offsetting yield gains. This study aimed to evaluate the two aforementioned modified wheat genotypes ( TaExpA6 and TaGW2 ) alongside their respective wild-type counterparts. Conducted in southern Chile, the study employed a Complete Randomized Block Design with four replications, under well-managed field conditions. The primary metrics assessed were grain yield, grain number, and average grain weight per spike, along with detailed measurements of grain weight and dimensions across the spike, ovary weight at pollination (Waddington’s scale 10), and post-anthesis expression levels of TaExpA6 and TaGW2 . Results indicated that both the TaExpA6 and the triple mutant lines achieved significantly higher average grain weights compared to their respective wild types. Notably, the TaExpA6 line did not exhibit a reduction in grain number, thereby enhancing grain yield per spike. By contrast, the triple mutant line showed a reduced grain number per spike, with no significant change in overall yield. TaExpA6 expression peaked at 10 days after anthesis (DAA), and its effect on grain weight over the WT became apparent after 15 DAA. In contrast, TaGW2 gene disruption in the triple mutant line increased ovary size at anthesis, leading to improved grain weight above the WT from the onset of grain filling. These findings suggest that the trade-off between grain weight and number could be attributed to the overlapping of the critical periods for the determination of these traits.

Introduction: In the last years, there has been a rise in the use of technology among children and adolescents, which has led to a greater concern about its impact on their socioemotional and cognitive development. Aims: To explore the time spent using technology, the risk perception of its use by students, and the association between frequency of technology use and life satisfaction and academic performance among children and adolescents in Chile. Additionally, we explored the mediating effect of sleep deprivation on these outcomes. Methods: This is a cross-sectional study, where 2,440 students (9-12 years old) from 13 schools participated. Data was collected using an online survey answered by students during school hours. The association analyses were performed using multivariable regression models considering life satisfaction and academic performance as dependent variables. Additionally, mediating analyses were conducted using structural equation modeling. Results: Time watching television and using cellphones were similar on weekdays, and playing video games was the most frequent activity during weekends. A 42.1% of students reported playing online video games with strangers, and 12.7% had sleep deprivation. Lower self-reported academic performance was associated with cyberbullying victimization, sleep deprivation, being hacked, exposure to violent content, time spent using cell phones during weekdays and weekends, and playing video games during weekdays. Students who perceived that playing video games after 9 pm affected their sleep had a higher academic performance. There was a clear mediating effect of sleep deprivation in the relationship between time spent using a cellphone during weekdays and weekends and playing video games during weekdays and GPA. Conclusions: Time spent using technological devices was not associated with life satisfaction; however, the time spent using cell phones and playing video games was related to lower self-reported academic performance, mediated by sleep deprivation. Future research may focus on a better understanding of the mechanisms involved in the effect of technology use on sleep routines among adolescents and potential interventions to reduce its impact on academic performance.

La economía de plataformas está reorganizando el trabajo y alterando la forma de concebirnos trabajadores. Control algorítmico, psicologización política y big data son parte de lo nuevo que define esta escena del trabajo. El trabajo en plataformas de reparto a domicilio, dirigido especialmente a jóvenes, está en el centro del debate acerca de la deslaboralización, a partir de la conflictividad que concentra internacionalmente. En este artículo exploramos el trabajo y cómo emerge la confrontación en estas aplicaciones, a partir del estudio del caso español, en un diseño de tipo etnográfico. Los resultados muestran la importancia de la exacerbación de los controles y la desprotección en la organización del trabajo, el desarrollo del activismo comunicacional por parte de los repartidores, y las disputas, también subjetivas, que se expresan alrededor de la cuestión de la laboralidad en la judicialización del conflicto. Se discuten los resultados a la luz de las tendencias de transformación neoliberal del trabajo.

BACKGROUND: The 6-minutes walking test (6WT) is the ideal submaximal test for the evaluation and follow-up of patients with chronic respiratory diseases. There are no reference values (RV) for Chilean children using the American Thoracic Society guidelines. AIM: To generate 6WT reference values for Chilean children aged 6 to 14 years. MATERIAL AND METHODS: 6MW was evaluated in 192 healthy children (100 women) aged between 6 and 14 years. The test was carried out in a 30 m long indoor flat surface. Children also answered a survey about health problems and their weight and height were recorded. RESULTS: The distance walked by women and men was 596.5 ± 57 and 625 ± 59.7 m respectively (p < 0.05). There was a significant correlation between the distance walked and height (r = 0.58), age (r = 0.56), weight (r = 0.54) and reserve heart rate (r = 0.21). CONCLUSIONS: These results can be used as reference values for the 6WT in Chilean children aged 6 to 14 years. They are similar to those reported abroad.

Fusulinids are unusual in the Southern Hemisphere. They are present in northern Brazil, Ecuador, Peru, and Bolivia to about lat 18 S. but are unknown in any of the marine deposits of late Paleozoic age between Bolivia and southern Chile. Fusulinids were recognized in 1952 in southern Chile in limestones quarried on Isla Guarello. In 1955, Cecioni reported the discovery of the fusulinids Millerella and Profusulinella from Isla Donas (lat 5043'S.) and a sequence from Fusulinella to Schwagerina from the archipelago Madre de Dios. The present study is based on additional samples from the islands of Guarello and Tarlton of this archipelago. The forms described are of Late Pennsylvanian and Early Permian age. A rather large fauna of fusulinids and other Foraminifera includes 11 genera and 24 species. Ten species are new, four are referred to forms previously described from the Bolivia and Peru areas, and the remainder are unassigned.

The geographical conditions in Chile make it a country that persistently experiences natural hazards, constantly testing the capacity of the Chilean society to pull itself up after every event and to improve the ways in which they occupy their territorial space. The prevalence of zoning that focus almost exclusively on profi tability and places little attention on localized risks, has allowed a majority of settlement projects and facilities to be built in areas that face signifi cant risk due to natural hazards. The aim of this work is to present a historical review of the subject, seeking to illustrate previous experiences in order to establish more sustainable forms of building within the Chilean geographical space.

Circular RNAs (circRNAs) are single-stranded and covalently closed non-coding RNA molecules originated from RNA splicing. Their functions include regulatory potential over other RNA species, such as microRNAs, messenger RNAs and RNA binding proteins. For circRNA identification, several algorithms are available and can be classified in two major types: pseudo-reference-based and split-alignment-based approaches. In general, the data generated from circRNA transcriptome initiatives is deposited on public specific databases, which provide a large amount of information on different species and functional annotations. In this review, we describe the main computational resources for the identification and characterization of circRNAs, covering the algorithms and predictive tools to evaluate its potential role in a particular transcriptomics project, including the public repositories containing relevant data and information for circRNAs, recapitulating their characteristics, reliability and amount of data reported.

Americanae nace como un proyecto conjunto que surge dentro de la Red Europea de Información y Documentación sobre América Latina (REDIAL), y que ha afrontado la Biblioteca de la Agencia Española de Cooperación Internacional para el Desarrollo (AECID). Esta nueva biblioteca virtual hace más accesibles los libros digitales de tema americanista a los investigadores y usuarios interesados de cualquier parte del mundo.

Surface winds along the subtropical west coast of South America are characterized by the quasi-weekly occurrences of low-level jet events. These short lived but intense wind events impact the coastal ocean environment. Hence, identifying long-term trends in the coastal low-level jet (CLLJ) is essential for understanding changes in marine ecosystems. Here we use ERA5 reanalysis (1979–2019) and an objective algorithm to track anticyclones to investigate recent changes in CLLJ events off central Chile (25–43 °S). Results present evidence that the number of days with intense wind (≥10 ms−1), and the number and duration of CLLJ events have significantly changed off central Chile in recent decades. There is an increase in the number of CLLJ events in the whole study area during winter (June-July-August; JJA), while during summer (December–January–February; DJF) a decrease is observed at lower latitudes (29–34 °S), and an increase is found at the southern boundary of the Humboldt system. We suggest that changes in the central pressures and frequency of extratropical, migratory anticyclones that reach the coast of South America, which force CLLJs, have played an important role in the recent CLLJ changes observed in this region.

Perinatal asphyxia (PA) is a leading cause of neuronal damage in newborns, resulting in long-term neurological and cognitive deficits, in part due to impairment of mesostriatal and mesolimbic neurocircuitries. The insult can be as severe as to menace the integrity of the genome, triggering the overactivation of sentinel proteins, including poly (ADP-ribose) polymerase-1 (PARP-1). PARP-1 overactivation implies increased energy demands, worsening the metabolic failure and depleting further NAD(+) availability. Using a global PA rat model, we report here evidence that hypoxia increases PARP-1 activity, triggering a signalling cascade leading to nuclear translocation of the NF-κB subunit p65, modulating the expression of IL-1β and TNF-α, pro-inflammatory molecules, increasing apoptotic-like cell death in mesencephalon of neonate rats, monitored with Western blots, qPCR, TUNEL and ELISA. PARP-1 activity increased immediately after PA, reaching a maximum 1-8 h after the insult, while activation of the NF-κB signalling pathway was observed 8 h after the insult, with a >twofold increase of p65 nuclear translocation. IL-1β and TNF-α mRNA levels were increased 24 h after the insult, together with a >twofold increase in apoptotic-like cell death. A single dose of the PARP-1 inhibitor nicotinamide (0.8 mmol/kg, i.p.), 1 h post delivery, prevented the effect of PA on PARP-1 activity, p65 translocation, pro-inflammatory cytokine expression and apoptotic-like cell death. The present study demonstrates that PA leads to PARP-1 overactivation, increasing the expression of pro-inflammatory cytokines and cell death in mesencephalon, effects prevented by systemic neonatal nicotinamide administration, supporting the idea that PARP-1 inhibition represents a therapeutic target against the effects of PA.

The development of metamorphic core complexes and associated low‐angle detachment faults commonly is intimately associated with synextensional igneous activity. In most areas studied to date, the relation of magmatism to extension is obscured by imprecise dating and by the overprint of later tectonic events. We present data from the early Miocene central Mojave metamorphic core complex (CMMCC) which indicate that extension was accompanied by igneous activity, as reflected by prekinematic, synkinematic, and postkinematic plutons and coeval volcanic rocks deposited in the associated extensional basins. The principal intrusion is an early Miocene granite pluton exposed in outcrops across an area greater than 400 km 2 . Dikes adjacent to the pluton are common in the Mitchel Range, at The Buttes, and at Fremont Peak. The overall orientation of the pluton and associated dikes is west‐northwest, roughly perpendicular to the extension direction. Results of U‐Pb analyses on zircon from two pluton and two dike samples yield ages of 20 to 23 Ma. Two other dike samples yield inconclusive results. Synextensional basins formed by detachment faulting during the core complex development. Rocks in these basins compose the Jackhammer and Pickhandle formations and filled an elongate, NW trending trough more than 50 km long. The 40 Ar/ 39 Ar ages for tuff beds are as old as 23.8±0.3 Ma near the base of the lower Pickhandle Formation and as young as 21.3±0.5 Ma in the uppermost lower Pickhandle. Hence volcanism and plutonism are coeval. The diversity of intrusive relations relative to the timing and development of the mylonitic fabric in the CMMCC precludes any simple cause‐and‐effect relationship between magmatism and extensional deformation. Rather, magmatism and extension may have been localized at a releasing bend in a transfer‐fault system which links extension in the CMMCC with extension in the Colorado River area to the east.