Central New York Research Corporation

The inferior parietal lobule (IPL) is a functionally and anatomically heterogeneous region that is concerned with multiple aspects of sensory processing and sensorimotor integration. Although considerable information is available about the corticocortical connections to the IPL, much less is known about the origin and importance of subcortical inputs to this cortical region. To examine this issue, we used retrograde transneuronal transport of the McIntyre-B strain of herpes simplex virus type 1 (HSV1) to identify the second-order neurons in subcortical nuclei that project to the IPL. Four monkeys (Cebus apella) received injections of HSV1 into three different subregions of the IPL. Injections into a portion of the lateral intraparietal area labeled second-order neurons primarily in the superficial (visual) layers of the superior colliculus. Injections of HSV1 into a portion of area 7a labeled many second-order neurons in the CA1 region of the hippocampus. In contrast, virus injections within a portion of area 7b labeled second-order neurons in posterior regions of the dentate nucleus of the cerebellum. These observations have some important functional implications. The IPL is known to be involved in oculomotor and attentional mechanisms, the establishment of maps of extrapersonal space, and the adaptive recalibration of eye-hand coordination. Our findings suggest that these functions are subserved by distinct subcortical systems from the superior colliculus, hippocampus, and cerebellum. Furthermore, the finding that each system appears to target a separate subregion of the IPL provides an anatomical substrate for understanding the functional heterogeneity of the IPL.

There is a growing need for new antibiotics. Compounds that target the proton motive force (PMF), uncouplers, represent one possible class of compounds that might be developed because they are already used to treat parasitic infections, and there is interest in their use for the treatment of other diseases, such as diabetes. Here, we tested a series of compounds, most with known antiinfective activity, for uncoupler activity. Many cationic amphiphiles tested positive, and some targeted isoprenoid biosynthesis or affected lipid bilayer structure. As an example, we found that clomiphene, a recently discovered undecaprenyl diphosphate synthase inhibitor active against Staphylococcus aureus, is an uncoupler. Using in silico screening, we then found that the anti-glioblastoma multiforme drug lead vacquinol is an inhibitor of Mycobacterium tuberculosis tuberculosinyl adenosine synthase, as well as being an uncoupler. Because vacquinol is also an inhibitor of M. tuberculosis cell growth, we used similarity searches based on the vacquinol structure, finding analogs with potent (∼0.5-2 μg/mL) activity against M. tuberculosis and S. aureus. Our results give a logical explanation of the observation that most new tuberculosis drug leads discovered by phenotypic screens and genome sequencing are highly lipophilic (logP ∼5.7) bases with membrane targets because such species are expected to partition into hydrophobic membranes, inhibiting membrane proteins, in addition to collapsing the PMF. This multiple targeting is expected to be of importance in overcoming the development of drug resistance because targeting membrane physical properties is expected to be less susceptible to the development of resistance.

Measurements of the thixotropic breakdown of structure with time of agitation were performed at different rates of shear on a number of pigment suspensions and on various oils in a rotational viscometer. The product of rate of breakdown in thixotropic structure and time of agitation at any rate of shear was found to be a constant for each material. It is called ``the time coefficient of thixotropic breakdown'' and is independent of the rate of shear, which is applied to agitate the sample. The time coefficient of thixotropic breakdown is measured in absolute units, having the dimensions of viscosity (dynes sec./cm2). The equilibrium time—the time necessary to break the thixotropic structure down to its minimum at a specified rate of shear—was also found to be independent of the rate of shear applied while agitating.

Abstract The main conclusions in studies on polymerization catalysts and the nature of chain transfer in the polymerization of cyclic imino ethers were that there is extensive chain transfer in the 2-alkyl oxazolines to produce polymer with a reactive end. Toward the end of polymerization, these chain-transferred molecules repolymerize back on the active center producing a multibranched star polymer. A theory for the data was developed.

The history of the esters of p -hydroxybenzoic acid and their uses as preservatives in pharmaceuticals, cosmetics, foods, and industrial products are discussed. Antimicrobial studies show that the methyl, ethyl, propyl, and butyl esters are effective in low concentrations against fungi and Gram-positive bacteria, but less effective against Gram-negative bacteria. The esters are more fungistatic than fungicidal. The p -hydroxybenzoates were also tested against microorganisms in the presence of humans serum, and against the growth of A. niger in procaine nicillin suspensions. The physical and chemical proprties of the p -hydroxygnwates are extensively described, as well as methods of incorporation and a method of determination by ultraviolet absorption.

Educator sexual misconduct has received increasing attention over the past decade. The attention has exposed a number of concerning issues, including a lack of formal research in the area and difficulties in recognizing and prosecuting cases. Public responses to high profile cases of sexual misconduct involving female teachers suggest that gender-biased views on sex offenders remain prominent in society. This article will review the literature on female teacher sexual misconduct in addition to what is known about grooming patterns and warning signs. Finally, current dilemmas in resolving cases of educator sexual misconduct will be discussed, and basic prevention strategies will be recommended.

Abstract This study examined food and beverage preferences of emergency food program (EFP) clients of the Food Bank of Central New York (Food Bank). Findings suggest that food pantry clients prefer to receive meat/poultry/fish, vegetables, and fruit over soda, candy, and snack foods. This new information supports the effort being made by the Food Bank to limit foods of minimal nutritional value while focusing on increasing distribution of donated and purchased fruits and vegetables. Limitations on the distribution of fresh fruits and vegetables in the emergency food program should be made on the basis of cost, availability, and infrastructure, rather than on the basis of perceived client or staff preferences to the contrary. Keywords: food insecurityemergency food programsfood policyhungerfood bank Acknowledgments The authors thank Thomas Slater, director, and the staff, pantry directors, and clients of the Food Bank of Central New York for their participation in this study; Shelly Mandel for data management assistance; and Lauren Goldstein and Sheila Stern for editorial review of the manuscript. A portion of the findings from this study were presented at the Institute of Medicine Workshop on Hunger and Obesity: Understanding a Food Insecurity Paradigm, November 17, 2010 (http://www.iom.edu/Home/Reports/2011/Hunger-and-Obesity-Understanding-a-Food-Insecurity-Paradigm.aspx). The study was funded by the Food Bank of Central New York.

New drugs to treat drug-resistant tuberculosis are urgently needed. Extensively drug-resistant and probably the totally drug-resistant tuberculosis strains are resistant to fluoroquinolones like moxifloxacin, which target gyrase A, and most people infected with these strains die within a year. In this study, we found that a novel aminobenzimidazole, VXc-486, which targets gyrase B, potently inhibits multiple drug-sensitive isolates and drug-resistant isolates of Mycobacterium tuberculosis in vitro (MICs of 0.03 to 0.30 μg/ml and 0.08 to 5.48 μg/ml, respectively) and reduces mycobacterial burdens in lungs of infected mice in vivo. VXc-486 is active against drug-resistant isolates, has bactericidal activity, and kills intracellular and dormant M. tuberculosis bacteria in a low-oxygen environment. Furthermore, we found that VXc-486 inhibits the growth of multiple strains of Mycobacterium abscessus, Mycobacterium avium complex, and Mycobacterium kansasii (MICs of 0.1 to 2.0 μg/ml), as well as that of several strains of Nocardia spp. (MICs of 0.1 to 1.0 μg/ml). We made a direct comparison of the parent compound VXc-486 and a phosphate prodrug of VXc-486 and showed that the prodrug of VXc-486 had more potent killing of M. tuberculosis than did VXc-486 in vivo. In combination with other antimycobacterial drugs, the prodrug of VXc-486 sterilized M. tuberculosis infection when combined with rifapentine-pyrazinamide and bedaquiline-pyrazinamide in a relapse infection study in mice. Furthermore, the prodrug of VXc-486 appeared to perform at least as well as the gyrase A inhibitor moxifloxacin. These findings warrant further development of the prodrug of VXc-486 for the treatment of tuberculosis and nontuberculosis mycobacterial infections.

Thirty-five people with work-related Multiple Chemical Sensitivities were studied to learn about the onset and progression of illness. The subjects were selected from patients at an occupational health clinic. Individuals were identified as subjects if they fulfilled a seven-point case definition for Multiple Chemical Sensitivities and if onset of symptoms was related to workplace exposures. Three occupational exposures to solvents, poor indoor-air quality, and remodeling were associated with onset of Multiple Chemical Sensitivities in 63% of the subjects. Symptoms indicative of a nervous-system disorder topped the list of the most frequently reported symptoms. Commonalities in exposures and symptoms suggest that Multiple Chemical Sensitivities represents a distinct diagnostic category. Even with an incomplete understanding of etiology, it may be possible to limit the onset of work-related Multiple Chemical Sensitivities.

The management of proximal fifth metatarsal ("Jones") fractures in athletes has become increasingly more aggressive, despite a lack of biomechanical data in the literature. A cadaver biomechanical study was conducted to evaluate the strength of intramedullary fixation of simulated Jones fractures loaded to failure via three-point bending on a Materials Testing System machine. In a series of eight intact fifth metatarsal control specimens, the force to failure (fracture) was measured for comparison with repaired specimens. Acute fractures were simulated in 10 pairs of feet via osteotomy at the typical fracture location and were fixed with either a 4.5-mm malleolar screw or a 4.5-mm partially threaded, cancellous, cannulated screw, both placed using conventional intramedullary techniques. Force at initial displacement averaged 73.9 N (SD, 64.7 N) for the malleolar screws and 72.5 N (SD, 42.3 N) for the cannulated screws. Force at complete displacement averaged 519.3 N (SD, 226.2 N) for the malleolar screws and 608.4 N (SD, 179.7 N) for the cannulated screws. The force to failure of the intact specimens was significantly greater than the initial and complete forces to failure for the fixed specimens (P < 0.05, independent measures analysis of variance). There was no statistical difference between the average forces at initial displacement or at complete displacement in the fixed metatarsal specimens for the two different types of screws, but the forces at complete displacement for each screw type were significantly greater than the forces at initial displacement (P < 0.05). On the basis of literature review and data generated from this study, it is apparent that the forces necessary to cause displacement of the stabilized Jones fracture are above what would be transmitted within the lateral midfoot during normal weightbearing. The choice of screw and intramedullary technique of fixation is a matter of surgeon preference, because the choice of screw makes no biomechanical difference.

This paper examines the relationship between self-efficacy and social power (expert and referent) and how the application of this relationship, leads to client adherence and compliance. Referent power is defined, including methods that health care professionals may use to develop and apply referent power. Expert power is defined and addressed in the context of referent power, self-esteem and self-efficacy as a means of promoting adherence. Self-efficacy as a concept is defined and explored in the context of social power. The relationships between self-efficacy and social power (expert and referent) are shown as important determinants of adherence and compliance. The theory of the application of referent and expert power in relationship with self-efficacy has been compared with an effective programme, yielding high compliance, at Western Psychiatric Institute and Clinic, showing the theory's relevance and applicability in determining compliance. Explored are the reasons for non-compliance in the elderly population and how the theory model can remedy these detriments for compliance. The empowerment of elderly patients through the application of this theory to medication compliance is examined. The determination of adherence and compliance is shown by the application of the relationship between self-efficacy and both expert and referent power.

The in vitro activity of omadacycline, a new tetracycline derivative, was evaluated against isolates of Mycobacterium abscessus , Mycobacterium chelonae , and Mycobacterium fortuitum using a broth microtiter dilution assay. Omadacycline had MIC 90 values of 2 μg/ml, 0.25 μg/ml, and 0.5 μg/ml, respectively.

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Carl E. Foss, Dorothy Nickerson, and Walter C. Granville, "Analysis of the Ostwald Color System*," J. Opt. Soc. Am. 34, 361-381 (1944) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article

Campylobacter enteritis is a food-borne or waterborne illness caused almost exclusively by Campylobacter jejuni and, to a lesser extent, by Campylobacter coli. These organisms produce indistinguishable clinical diseases and together represent the second most common cause of bacterial diarrhea in the United States and the leading cause of enteric infection throughout the world. The conventional approach to the laboratory diagnosis of Campylobacter enteritis is based on the recovery of the organism from a stool specimen, which requires the use of a specialized medium incubated at 42°C for several days in an artificially created microaerophilic environment. Recently, several commercially available enzyme immunoassays (EIAs) have been developed for the direct detection of C. jejuni and C. coli in stool specimens. This study compared conventional culture with three EIA methods, the Premier CAMPY EIA (Meridian Bioscience, Cincinnati, OH), the ProSpecT Campylobacter EIA (Remel, Lenexa, KS), and the ImmunoCard STAT! CAMPY test (Meridian Bioscience, Cincinnati, OH), for the detection of C. jejuni and C. coli in 485 patient stool samples. Discordant results were arbitrated by using an in-house, real-time PCR assay that was developed and validated by a public health reference laboratory. Following analyses of the discrepant specimens by PCR, the sensitivity and specificity of both the Premier CAMPY and ProSpecT Campylobacter EIAs were 99.3% and 98%, respectively, while the ImmunoCard STAT! CAMPY test had a sensitivity of 98.5% and a specificity of 98.2%. By use of the PCR test as the reference standard, culture detected 127 of 135 Campylobacter-positive stool specimens, yielding a sensitivity of 94.1%. These results showed that the three EIAs evaluated in this study provide a rapid and reliable alternative for the laboratory diagnosis of enteric infections with C. jejuni and C. coli and that conventional culture may no longer be recognized as the "gold standard" for diagnosis.

ABSTRACT The in vitro activity of contezolid (MRX-I) against clinical isolates of Mycobacterium tuberculosis was evaluated using a microtiter broth dilution assay. MRX-I was as effective as linezolid (LZD) in vitro . MRX-I and LZD were subsequently studied in BALB/c mice infected intranasally with M. tuberculosis Erdman. MRX-I and LZD at 100 mg/kg significantly reduced the bacterial load in lungs compared to the untreated early and late controls.

Abstract: The processes of finding a solution to a stated problem and those employed in formulating a problem prior to determining a solution strategy are implied by the behaviors observed in the manipulation of figural symbol systems. Sixty adult participants agreed to be videotaped while given two measures of spatial visualization, a figural problem‐solving activity, and two games which required defining a problem prior to producing a result. When groups, defined by their experience in producing ideas in art, were compared, traditional quantitative measures of performance yielded few significant differences. However, multivariate analyses of the observed qualitative variables resulted in significant differences. Theoretical and educational implications are discussed.

Abstract The specific volume of a polyvinyl chloride‐polyvinyl acetate copolymer was studied as a function of temperature. It was found that two second‐order transition temperatures exist, one at 30°C. and the other at 77°C. The lower transition temperature has not been reported previously. Diffusion coefficients for several gases of varying van der Waals diameters were measured at several different temperatures and the activation energies for diffusion calculated. It was found that the activation energies were exponential functions of the gas diameters. For the smaller‐sized gases, plots of log diffusion coefficient against 1/ T unexpectedly did not change slope at either of the two transition temperatures. On the other hand, the transport processes for carbon dioxide and water vapor were affected by the glass transition temperatures of the copolymer. For the former the slope changed at the lower transition temperature of 30°C. In contrast, the water vapor diffusion coefficients increased normally with temperature until the second transition temperature of 77°C. was reached. At this point the diffusion coefficients exhibited a marked increase with temperature. An explanation for the anomalously high diffusion values for water vapor is offered.

Two forms of a high speed type of microtome are described which are able to cut cross sections to the thinness required for electron microscopic study (0.1 to 0.8 micron, depending on the material). Techniques of microtome operation are suggested together with a few methods of sample preparation and subsequent section treatment, namely, collecting, selecting, and mounting. Embedding materials which sublime readily are described; these have been used successfully with this high speed microtome to support many types of materials. These volatile embedding materials have the advantage that they eliminate the difficulties that on some occasions arise with the use of solvents in the process of solvent extraction of embedding materials, such as Carbowax and paraffin, from the sections. A number of photographs are shown of the high speed microtome as well as electron micrographs of a few sections produced by the instrument. These pictures of rubber, rayon, Lucite, block Nylon, and animal tissue indicate some of the fields of application of the microtome and demonstrate the effectiveness of the necessary auxiliary techniques for it, particularly sample hardening and embedding, and section collecting and mounting.

Abstract Norbornene and a derivative have been polymerized to high molecular weight materials by use of the hydrated trichlorides of ruthenium, osmium, and iridium in alcoholic solvents. Structural studies on these polymers indicate that a major portion of the polymer repeating unit consists of cyclopentane rings linked in cis ‐1,3 configuration with a predominance of trans double bonds.

Peptide deformylase (PDF) catalyzes the hydrolytic removal of the N-terminal formyl group from nascent proteins. This is an essential step in bacterial protein synthesis, making PDF an attractive target for antibacterial drug development. Essentiality of the def gene, encoding PDF from Mycobacterium tuberculosis, was demonstrated through genetic knockout experiments with Mycobacterium bovis BCG. PDF from M. tuberculosis strain H37Rv was cloned, expressed, and purified as an N-terminal histidine-tagged recombinant protein in Escherichia coli. A novel class of PDF inhibitors (PDF-I), the N-alkyl urea hydroxamic acids, were synthesized and evaluated for their activities against the M. tuberculosis PDF enzyme as well as their antimycobacterial effects. Several compounds from the new class had 50% inhibitory concentration (IC50) values of <100 nM. Some of the PDF-I displayed antibacterial activity against M. tuberculosis, including MDR strains with MIC90 values of <1 microM. Pharmacokinetic studies of potential leads showed that the compounds were orally bioavailable. Spontaneous resistance towards these inhibitors arose at a frequency of < or =5 x 10(-7) in M. bovis BCG. DNA sequence analysis of several spontaneous PDF-I-resistant mutants revealed that half of the mutants had acquired point mutations in their formyl methyltransferase gene (fmt), which formylated Met-tRNA. The results from this study validate M. tuberculosis PDF as a drug target and suggest that this class of compounds have the potential to be developed as novel antimycobacterial agents.