Franche-Comté Électronique Mécanique Thermique et Optique - Sciences et Technologies

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

A topical review of numerical and experimental studies of supercontinuum generation in photonic crystal fiber is presented over the full range of experimentally reported parameters, from the femtosecond to the continuous-wave regime. Results from numerical simulations are used to discuss the temporal and spectral characteristics of the supercontinuum, and to interpret the physics of the underlying spectral broadening processes. Particular attention is given to the case of supercontinuum generation seeded by femtosecond pulses in the anomalous group velocity dispersion regime of photonic crystal fiber, where the processes of soliton fission, stimulated Raman scattering, and dispersive wave generation are reviewed in detail. The corresponding intensity and phase stability properties of the supercontinuum spectra generated under different conditions are also discussed.

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.

The global energy transition towards a carbon neutral society requires a profound transformation of electricity generation and consumption, as well as of electric power systems. Hydrogen has an important potential to accelerate the process of scaling up clean and renewable energy, however its integration in power systems remains little studied. This paper reviews the current progress and outlook of hydrogen technologies and their application in power systems for hydrogen production, re-electrification and storage. The characteristics of electrolysers and fuel cells are demonstrated with experimental data and the deployments of hydrogen for energy storage, power-to-gas, co- and tri-generation and transportation are investigated using examples from worldwide projects. The current techno-economic status of these technologies and applications is presented, in which cost, efficiency and durability are identified as the main critical aspects. This is also confirmed by the results of a statistical analysis of the literature. Finally, conclusions show that continuous efforts on performance improvements, scale ramp-up, technical prospects and political support are required to enable a cost-competitive hydrogen economy.

1Deriving ice thickness, glacier volume and bedrock morphology of the Austre Lovénbreen (Svalbard) using

In this framework, the demonstration of planar high-Q resonators, compatible with silicon technology Indeed, it is well acknowledged by the electronics industry that future generations of computer processing chips will inevitably require an extremely high density of copper-based interconnections, significantly increasing the chip power dissipation to beyond practical levels On-chip optical networks, or optical interconnects, can offer high speed and low energy pertransferred-bit, and micro-resonators are widely seen as a key component to interface the electronic world with photonics.

Rationally designed artificial materials enable mechanical properties that are inaccessible with ordinary materials. Pushing on an ordinary linearly elastic bar can cause it to be deformed in many ways. However, a twist, the counterpart of optical activity in the static case, is strictly zero. The unavailability of this degree of freedom hinders applications in terms of mode conversion and the realization of advanced mechanical designs using coordinate transformations. Here, we aim at realizing microstructured three-dimensional elastic chiral mechanical metamaterials that overcome this limitation. On overall millimeter-sized samples, we measure twists per axial strain exceeding 2°/%. Scaling up the number of unit cells for fixed sample dimensions, the twist is robust due to metamaterial stiffening, indicating a characteristic length scale and bringing the aforementioned applications into reach.

Myeloid-derived suppressor cells (MDSCs) have been identified in humans and mice as a population of immature myeloid cells with the ability to suppress T cell activation. They accumulate in tumor-bearing mice and humans and have been shown to contribute to cancer development. Here, we have isolated tumor-derived exosomes (TDEs) from mouse cell lines and shown that an interaction between TDE-associated Hsp72 and MDSCs determines the suppressive activity of the MDSCs via activation of Stat3. In addition, tumor-derived soluble factors triggered MDSC expansion via activation of Erk. TDE-associated Hsp72 triggered Stat3 activation in MDSCs in a TLR2/MyD88-dependent manner through autocrine production of IL-6. Importantly, decreasing exosome production using dimethyl amiloride enhanced the in vivo antitumor efficacy of the chemotherapeutic drug cyclophosphamide in 3 different mouse tumor models. We also demonstrated that this mechanism is relevant in cancer patients, as TDEs from a human tumor cell line activated human MDSCs and triggered their suppressive function in an Hsp72/TLR2-dependent manner. Further, MDSCs from cancer patients treated with amiloride, a drug used to treat high blood pressure that also inhibits exosome formation, exhibited reduced suppressor functions. Collectively, our findings show in both mice and humans that Hsp72 expressed at the surface of TDEs restrains tumor immune surveillance by promoting MDSC suppressive functions.

Many information processing challenges are difficult to solve with traditional Turing or von Neumann approaches. Implementing unconventional computational methods is therefore essential and optics provides promising opportunities. Here we experimentally demonstrate optical information processing using a nonlinear optoelectronic oscillator subject to delayed feedback. We implement a neuro-inspired concept, called Reservoir Computing, proven to possess universal computational capabilities. We particularly exploit the transient response of a complex dynamical system to an input data stream. We employ spoken digit recognition and time series prediction tasks as benchmarks, achieving competitive processing figures of merit.

International audience

The optical fiber based supercontinuum source has recently become a significant scientific and commercial success, with applications ranging from frequency comb production to advanced medical imaging. This one-of-a-kind book explains the theory of fiber supercontinuum broadening, describes the diverse operational regimes and indicates principal areas of applications, making it a very important guide for researchers and graduate students. With contributions from major figures and groups who have pioneered research in this field, the book describes the historical development of the subject, provides a background to the associated nonlinear optical processes, treats the generation mechanisms from continuous wave to femtosecond pulse pump regimes and highlights the diverse applications. A full discussion of numerical methods and comprehensive computer code are also provided, enabling readers to confidently predict and model supercontinuum generation characteristics under realistic conditions.

Abstract We review a novel paradigm that has emerged in analogue neuromorphic optical computing. The goal is to implement a reservoir computer in optics, where information is encoded in the intensity and phase of the optical field. Reservoir computing is a bio‐inspired approach especially suited for processing time‐dependent information. The reservoir’s complex and high‐dimensional transient response to the input signal is capable of universal computation. The reservoir does not need to be trained, which makes it very well suited for optics. As such, much of the promise of photonic reservoirs lies in their minimal hardware requirements, a tremendous advantage over other hardware‐intensive neural network models. We review the two main approaches to optical reservoir computing: networks implemented with multiple discrete optical nodes and the continuous system of a single nonlinear device coupled to delayed feedback.

We demonstrate experimentally the guiding and the bending of acoustic waves in highly confined waveguides formed by removing rods from a periodic two-dimensional lattice of steel cylinders immersed in water. Full transmission is observed for a one-period-wide straight waveguide within the full band gap of the perfect phononic crystal. However, when the waveguide width is doubled, destructive interference causes the transmission to vanish in the center of the passband. Waveguiding over a wide frequency range is obtained for a one-period-wide waveguide with two sharp 90° bends. Finite-difference time-domain computations are found to be in good agreement with the measurements.

Numerical simulations of the onset phase of continuous wave supercontinuum generation from modulation instability show that the structure of the field as it develops can be interpreted in terms of the properties of Akhmediev Breathers. Numerical and analytical results are compared with experimental measurements of spectral broadening in photonic crystal fiber using nanosecond pulses.

Reservoir computing, originally referred to as an echo state network or a liquid state machine, is a braininspired paradigm for processing temporal information. It involves learning a "read-out" interpretation for nonlinear transients developed by high-dimensional dynamics when the latter is excited by the information signal to be processed. This novel computational paradigm is derived from recurrent neural network and machine learning techniques. It has recently been implemented in photonic hardware for a dynamical system, which opens the path to ultrafast brain-inspired computing. We report on a novel implementation involving an electro-optic phase-delay dynamics designed with off-the-shelf optoelectronic telecom devices, thus providing the targeted wide bandwidth. Computational efficiency is demonstrated experimentally with speech-recognition tasks. State-of-the-art speed performances reach one million words per second, with very low word error rate. Additionally, to record speed processing, our investigations have revealed computing-efficiency improvements through yet-unexplored temporalinformation-processing techniques, such as simultaneous multisample injection and pitched sampling at the read-out compared to information "write-in".

We demonstrate that frequency (Kerr) comb generation in whispering-gallery-mode resonators can be modeled by a variant of the Lugiato-Lefever equation that includes higher-order dispersion and nonlinearity. This spatiotemporal model allows us to explore pulse formation in which a large number of modes interact cooperatively. Pulse formation is shown to play a critical role in comb generation, and we find conditions under which single pulses (dissipative solitons) and multiple pulses (rolls) form. We show that a broadband comb is the spectral signature of a dissipative soliton, and we also show that these solitons can be obtained by using a weak anomalous dispersion and subcritical pumping.

With the advent of quantum key distribution (QKD) systems, perfect (i.e., information-theoretic) security can now be achieved for distribution of a cryptographic key. QKD systems and similar protocols use classical error-correcting codes for both error correction (for the honest parties to correct errors) and privacy amplification (to make an eavesdropper fully ignorant). From a coding perspective, a good model that corresponds to such a setting is the wire tap channel introduced by Wyner in 1975. In this correspondence, we study fundamental limits and coding methods for wire tap channels. We provide an alternative view of the proof for secrecy capacity of wire tap channels and show how capacity achieving codes can be used to achieve the secrecy capacity for any wiretap channel. We also consider binary erasure channel and binary symmetric channel special cases for the wiretap channel and propose specific practical codes. In some cases our designs achieve the secrecy capacity and in others the codes provide security at rates below secrecy capacity. For the special case of a noiseless main channel and binary erasure channel, we consider encoder and decoder design for codes achieving secrecy on the wiretap channel; we show that it is possible to construct linear-time decodable secrecy codes based on low-density parity-check (LDPC) codes that achieve secrecy.

A new approach to compensate the strong hysteresis nonlinearity in piezoelectric materials is proposed. Based on the inverse multiplicative scheme, the approach avoids models inversion as employed in existing works. The compensator is therefore simple to implement and does not require additional computation as soon as the direct model is available. The proposed compensation technique is valuable for hysteresis that are modeled with the Bouc-Wen set of equations.

We propose a detailed stability analysis of the Lugiato-Lefever model for Kerr optical frequency combs in whispering-gallery-mode resonators when they are pumped in either the anomalous- or normal-dispersion regime. We analyze the spatial bifurcation structure of the stationary states depending on two parameters that are experimentally tunable; namely, the pump power and the cavity detuning. Our study demonstrates that, in both the anomalous- and normal-dispersion cases, nontrivial equilibria play an important role in this bifurcation map because their associated eigenvalues undergo critical bifurcations that are actually foreshadowing the existence of localized and extended spatial dissipative structures. The corresponding bifurcation maps are evidence of a considerable richness from a dynamical standpoint. The case of anomalous dispersion is indeed the most interesting from the theoretical point of view because of the considerable variety of dynamical behavior that can be observed. For this case we study the emergence of super- and subcritical Turing patterns (or primary combs) in the system via modulational instability. We determine the areas where bright isolated cavity solitons emerge, and we show that soliton molecules can emerge as well. Very complex temporal patterns can actually be observed in the system, where solitons (or soliton complexes) coexist with or without mutual interactions. Our investigations also unveil the mechanism leading to the phenomenon of breathing solitons. Two routes to chaos in the system are identified; namely, a route via the destabilization of a primary comb, and another via the destabilization of solitons. For the case of normal dispersion, we unveil the mechanism leading to the emergence of weakly stable Turing patterns. We demonstrate that this weak stability is justified by the distribution of stable and unstable fixed points in the parameter space (flat states). We show that dark cavity solitons can emerge in the system, and also show how these solitons can coexist in the resonator as long as they do not interact with each other. We find evidence of breather solitons in this normal dispersion regime as well. The Kerr frequency combs corresponding to all these spatial dissipative structures are analyzed in detail, along with their stability properties. A discussion is led about the possibility to gain unifying comprehension of the observed spectra from the dynamical complexity of the system.

Presenting a comprehensive account of oscillator phase noise and frequency stability, this practical text is both mathematically rigorous and accessible. An in-depth treatment of the noise mechanism is given, describing the oscillator as a physical system, and showing that simple general laws govern the stability of a large variety of oscillators differing in technology and frequency range. Inevitably, special attention is given to amplifiers, resonators, delay lines, feedback, and flicker (1/f) noise. The reverse engineering of oscillators based on phase-noise spectra is also covered, and end-of-chapter exercises are given. Uniquely, numerous practical examples are presented, including case studies taken from laboratory prototypes and commercial oscillators, which allow the oscillator internal design to be understood by analyzing its phase-noise spectrum. Based on tutorials given by the author at the Jet Propulsion Laboratory, international IEEE meetings, and in industry, this is a useful reference for academic researchers, industry practitioners, and graduate students in RF engineering and communications engineering.