General Administration of Sport of China

China is experiencing significant public health challenges related to social and demographic transitions and lifestyle transformations following unprecedented economic reforms four decades ago. Of particular public health concern is the fourfold increase in overweight and obesity rates in the nation’s youth population, coupled with the low prevalence of adolescents meeting recommended levels of physical activity. Improving the overall health of China’s more than 170 million children and adolescents has become a national priority. However, advancing nationwide health initiatives and physical activity promotion in this population has been hampered by the lack of a population-specific and culturally relevant consensus on recommendations for achieving these ends. To address this deficiency and inform policies to achieve Healthy China 2030 goals, a panel of Chinese experts, complemented by international professionals, developed this consensus statement. The consensus was achieved through an iterative process that began with a literature search from electronic databases; in-depth reviews, conducted by a steering committee, of the resulting articles; and panel group evaluations and discussions in the form of email correspondence, conference calls and written communications. Ultimately, the panel agreed on 10 major themes with strong scientific evidence that, in children and adolescents aged 6–17, participating in moderate to vigorous physical activities led to multiple positive health outcomes. Our consensus statement also (1) highlights major challenges in promoting physical activity, (2) identifies future research that addresses current knowledge gaps, and (3) provides recommendations for teachers, education experts, parents and policymakers for promoting physical activity among Chinese school-aged children and adolescents. This consensus statement aligns with international efforts to develop global physical activity guidelines to promote physical activity and health and prevent lifestyle-related diseases in children and adolescents. More importantly, it provides a foundation for developing culturally appropriate and effective physical activity interventions, health promotion strategies and policy initiatives to improve the health of Chinese children and adolescents.

Nature exposure is known to promote physical and mental health. However, actual nature exposure may be difficult to achieve for the population of people with physical disabilities or chronic conditions. Therefore, many attempts have been made to duplicate nature exposure via media devices, and virtual reality (VR) is deemed as a promising technology due to its advantage in creating a sense of immersion. Generally, current studies suggest that being exposed to virtual nature may contribute to psychological and physiological relaxation. Besides, some pieces of evidence indicate that virtual nature may improve attentional resources, cognitive performance, and pain experience. Although VR is deemed as an advanced media, insufficient evidence was found concerning the advantages of VR over traditional two-dimensional media when it comes to simulated nature exposure. On the other hand, computer-generated (CG) scenarios were found to be more beneficial than 360° videos, and mini-games may be useful in creating an interactive VR format for simulated nature exposure. Further research is needed because of the limited relevant studies.

Purpose: This study aimed to compare the response to acute exercise of several myokines/hormones involved in metabolic function between two types of training sessions that are growing in popularity for their purported cardiometabolic benefits, high-intensity interval (HIIT) and resistance training (RT). Methods: Seventeen healthy, non-athletic men (23±3 years) participated in this cross-over study. They randomly performed a HIIT (with short (HIIT1) or long (HIIT2) intervals) or a RT session. The concentration of fibroblast-growth factor (FGF)21, follistatin, myostatin, interleukin-15, irisin, ghrelin and peptide YY was measured at baseline and 0, 1, 3, 24, 48 and 72 hours post-exercise. An individual approach was adopted to determine the rate of responsiveness to each specific cytokine and training mode. Results: A significant condition (session type) by time interaction (p=0.004) effect was observed for FGF21, with RT eliciting a greater area under the curve (AUC) concentration than HIIT1 (p=0.02). The AUC for follistatin was significantly greater after HIIT2 compared with RT (p=0.02). Individual responsiveness to all session types ranged between 19–93% depending on the cytokine. However, most subjects (71–100%) responded positively for all cytokines (except for irisin, with only 53% of responders) after 1+ session type. Conclusions: Except for FGF21, our results show no overall differences in the myokine response to HIIT or RT. A considerable individual variability was observed, with some subjects responding to some but not other training session types. Notwithstanding, most responded to at least one training session. Thus, it is mostly the individual response of each subject rather than general recommendations on type of training session (i.e., RT vs HIIT or HIIT subtypes) that must be taken into consideration for maximizing cardiometabolic benefits in the context of personalized exercise prescription.

Mitochondrial biogenesis is a key factor, which influences the function of skeletal muscle. Increasingly, flavonoids are reported to have the potential ability of regulating mitochondrial biogenesis. In this study, we investigated the effects of tangeretin, a polymethoxylated flavonoid isolated from mandarin fruits, on mitochondrial biogenesis and its underlying mechanisms. The tangeretin was obtained from the peel of "Dahongpao" tangerine by macroporous adsorptive resins combined with preparative-high performance liquid chromatography. The activity of mitochondrial biogenesis was explored by using mouse-derived C2C12 myoblasts and Kunming mice. Results showed that the purity of tangeretin obtained was 98.64%, and it could effectively activate mitochondrial biogenesis signaling pathway both at gene and at protein levels in C2C12 myoblasts. Animal experiments showed that tangeretin pretreatment could markedly improve exercise performance (the time of hanging wire and run to fatigue was obviously increased 1.6-fold and 2.1-fold in the high-dose tangeretin group, respectively), and the transmission electron microscopy, Western blotting, and immunohistochemistry further indicated that tangeretin increased mitochondria number and activated mitochondrial biogenesis signaling axis. Our findings suggest that tangeretin enhanced mitochondrial biogenesis via activating the AMPK-PGC1-α pathway, resulting in the improvement of exercise performance, and tangeretin may be a potentially novel mitochondria regulator in foods.

The focus of this study was threefold: 1) to create a comprehensive list of possible structural constraints to attending a sport event; 2) to create categories of structural constraints; and 3) to determine whether males differed from females and whether attendees differed from non-attendees on structural constraints of sport attendance. Thirteen different structural constraint dimensions were identified from factor analysis. There were significant and meaningful differences by gender. Males perceived that the opportunity for other sport entertainment, and lack of team success, were greater constraints to attending games than females. Females felt that poor weather was a bigger constraint than males. Thus, managers and marketers should show that the game is a better entertainment alternative than other sport events and also realize that if the team is not successful, those who are only spectators will need other incentives to come.

Self‐powered wearable devices have attracted significantly increased attention in human motion monitoring. These flexible physical sensors conformally attach to the surface of the skin to provide new applications in human‐activity monitoring. Herein, a highly flexible and self‐powered piezoelectric pressure sensor for real‐time human motion detecting using a piezoelectric thin film with high filling ratio of lead zirconate titanate (PZT) nanofibers (NFs) in polydimethylsiloxane (PDMS) is reported. Due to the large dielectric constant of PZT and elastic properties of PDMS, the sensor performs in a wide linear region (1.25–250 kPa) with great linearity (pressure–voltage 0.9909) and good reproducibility over 2000 cycles. Using the sensor, various human body motions are detected, including joint bending, subtle/large wrist deformations, wrist and some common sporting movements. In addition, a sensor array is successfully developed to realize motion tracking, showing strong potential for application in personal recognition.

Background: It is well established that greater internet use is related to an increased risk of musculoskeletal pain among adolescents. The relationship between internet addiction, a unique condition involving severe internet overuse, and musculoskeletal pain has, however, not been reported. The aim of this study was to examine the association between internet addiction and the risk of musculoskeletal pain in different body parts among Chinese college freshmen. Methods: A cross-sectional study was conducted among 4211 Chinese college freshmen. Internet addiction status was evaluated using the 20-item Young's Internet Addiction Test. Internet addiction was defined as internet addiction score ≥ 50. Musculoskeletal pain was assessed using a self-report questionnaire. Results: Among all subjects, 29.2% reported neck pain, 33.9% shoulder pain, 3.8% elbow pain, 7.9% wrist/hand pain, and 27.9% low back and waist pain. The prevalence of internet addiction was 17.4%. After adjusting for potential confounders, internet addiction was significantly associated with risk of neck, shoulder, elbow, wrist/hand, low back and waist pain. The odds ratios and 95% confidence intervals (CI) for neck pain with severe internet addiction status were 1.00 (reference), 1.451 (1.221, 1.725), and 1.994 (1.608, 2.473) (P for trends: <0.001). The odds ratios (95% CI) for shoulder pain with severe internet addiction status were 1.00 (reference), 1.520 (1.287, 1.795), and 2.057 (1.664, 2.542) (P for trends: <0.001). The odds ratios (95% CI) for elbow pain with severe internet addiction were 1.00 (reference), 1.627 (1.016, 2.605), and 2.341 (1.382, 3.968) (P for trends: 0.001). The odds ratios (95% CI) for wrist/hand pain with severe internet addiction status were 1.00 (reference), 1.508 (1.104, 2.060), and 2.236 (1.561, 3.202) (P for trends: <0.001). The odds ratios (95% CI) for low back and waist pain with severe internet addiction status were 1.00 (reference), 1.635 (1.368, 1.955), and 2.261 (1.813, 2.819) (P for trends: <0.001). Conclusion: This cross-sectional study showed that severe internet addiction is significantly associated with a higher risk of musculoskeletal pain in Chinese college freshmen. In future research, it will be necessary to explore causality in this relationship with interventional studies.

We examined the influence of vicarious achievement and team identification on BIRGing and CORFing behavior. We tested three different models (direct effects, partially mediated, and fully mediated) across two different situations: BIRGing with the winning team and CORFing with the losing team. Data were collected from 246 students. The fully mediated model fit best in the BIRGing situation and the partially mediated model fit best in the CORFing situation. We found that vicarious achievement explained 12.7% to 16.9% of the variance in team identification across situations. Vicarious achievement explained approximately 17% of the variance in CORFing behavior, but none in BIRGing. Team identification explained 30% of the variance in CORFing behavior and 41% in BIRGing. It is critical for sport teams to develop a high level of identification in their spectators. High identification makes it less likely that people will CORF and more likely that people will BIRG.

Aggressive behavior has been one of the core issues in sports psychology, whereas boxers’ aggressive behavior has received limited attention. Although some literature reported that self-efficacy is related to aggressive behavior, the mechanism whereby self-efficacy affects aggressive behavior remains unclear. The present study investigated the relationship between self-efficacy and aggressive behavior, as well as the effect of self-control as a mediating factor. This study uses the Self-efficacy Scale for Athletes, the Self-control Questionnaire for Athletes, and the Buss-Perry Aggression Questionnaire. This relationship is explored through self-reported measures from N= 414 Chinese professional boxers, n = 243 were male and n = 171 were female, the average age was M=17.72 years (SD = 3.147), the participants, the average number of years of exercise was M=3.89 years (SD = 2.734); Results showed that male boxers reported greater aggression than female boxers; It was found that the self-efficacy and self-control improved as age of the participants increased; The higher the level of competition, the higher levels of self-efficacy and self-control; Self-efficacy was negatively related with aggressive behavior and positively correlated with self-control. Self-control was also negatively correlated with aggressive behavior among boxers. Self-control had a full mediating effect on the relationship between self-efficacy and aggressive behavior.

Cardiac troponin release is generally found in adult athletes after continuous-type endurance exercises or sport competitions. The purpose of this study was to investigate whether the physical stress experienced by adolescents while playing basketball, an intense, intermittent-type sport, could induce transient elevations of the serum cardiac troponin T (cTnT) and I (cTnI). Serum cTnT and cTnI levels in 10 male adolescent players (age 15.0 +/- 0.7 yr) were assessed immediately before and at 2, 4 and 24 h after a game randomly selected from a preseason basketball-training program. At 4 h following the game, serum cTnT levels in four of the ten subjects were above the cutoff of 0.01 ng . ml (-1) for myocardial injury. Two of these four subjects had values higher than the acute myocardial infarction cutoff of 0.05 ng . ml (-1). In three of the four subjects, the serum cTnI was above the cutoff of 0.06 ng . ml (-1) for myocardial injury. Nevertheless, serum cardiac troponins at 24 h had returned to pre-exercise levels. These findings suggest that the physical stress encountered during intense, intermittent-type sports could cause release of cardiac troponins in some adolescents at low risk for cardiac disease.

Objective: Infiltrated macrophages actively promote perivascular adipose tissue remodeling and represent a dominant population in the perivascular adipose tissue microenvironment of hypertensive mice. However, the role of macrophages in initiating metabolic inflammation remains uncertain. SIRT3 (sirtuin-3), a NAD-dependent deacetylase, is sensitive to metabolic status and mediates adaptation responses. In this study, we investigated the role of SIRT3-mediated metabolic shift in regulating NLRP3 (Nod-like receptor family pyrin domain-containing 3) inflammasome activation. Approach and Results: Here, we report that Ang II (angiotensin II) accelerates perivascular adipose tissue inflammation and fibrosis, accompanied by NLRP3 inflammasome activation and IL (interleukin)-1β secretion in myeloid SIRT3 knockout (SIRT3 −/ − ) mice. This effect is associated with adipose tissue mitochondrial dysfunction. In vitro studies indicate that the deletion of SIRT3 in bone marrow–derived macrophages induces IL-1β production by shifting the metabolic phenotype from oxidative phosphorylation to glycolysis. Mechanistically, SIRT3 deacetylates and activates PDHA1 (pyruvate dehydrogenase E1 alpha) at lysine 83, and the loss of SIRT3 leads to PDH activity decrease and lactate accumulation. Knocking down LDHA (lactate dehydrogenase A) or using carnosine, a buffer against lactic acid, attenuates IL-1β secretion. Furthermore, the blockade of IL-1β from macrophages into brown adipocytes restores thermogenic markers and mitochondrial oxygen consumption. Moreover, NLRP3 knockout (NLRP3 −/− ) mice exhibited reduced IL-1β production while rescuing the mitochondrial function of brown adipocytes and alleviating perivascular adipose tissue fibrosis. Conclusions: SIRT3 represents a potential therapeutic target to attenuate NLRP3-related inflammation. Pharmacological targeting of glycolytic metabolism may represent an effective therapeutic approach.

Purpose: Exercise health benefits are partly mediated by exertional changes in several myokines/adipokines. but little is known on individual variability, which is an important question in the context of personalized exercise prescription. This study aimed to compare the acute response of some of these biomarkers to aerobic exercise performed at the intensity corresponding to the maximum fat oxidation rate (FATmax) or the ‘anaerobic’ threshold (AT). Methods: Following a cross-over, counterbalanced design, 14 healthy untrained men (23±1 years) performed a 45-minute exercise bout at their FATmax or AT intensity (been previously determined through incremental exercise tests). The concentration of interleukin (IL)-15, follistatin, myostatin, fibroblast-growth factor (FGF)-21, irisin, resistin and omentin was measured at baseline and 0, 1, 3, 24, 48 and 72 hours post-exercise. Results: AT exercise was performed at a higher intensity (85±8 vs 52±14% of maximal oxygen uptake [VO2max], p0.05). Conclusion: In summary, Iincreasing exercise intensity load (from FATmax to AT) maximizes results in a higher response of the FGF-21, follistatin and omentin response to aerobic exercise, with the subsequent potential cardiometabolic benefits. No intensity effects were, however, observed on the remainder of biomarkers. or on the rate of responsiveness. Future research should address if manipulating other exercise variables (e.g., type, frequency) can promote a higher myokine/adipokine individual responsivenessresponse.

The aim of the present study was to examine physical fitness differences between Freestyle and Greco-Roman junior wrestlers. One hundred twenty-six junior wrestlers, comprising 70 Freestyle and 56 Greco-Roman wrestlers, participated in this study. The somatic and physical fitness profile included body mass, body height, body mass index, body composition, flexibility, maximal anaerobic power of the legs and arms, aerobic endurance, hand grip strength, leg and back strength, and speed. No significant differences were found in the anthropometric and physical features between Freestyle and Greco-Roman wrestlers. The Greco-Roman wrestlers had a significantly higher level of relative leg power, peak arm power, relative peak arm power, and relative average arm power than Freestyle wrestlers (p < 0.05). Greco-Roman wrestlers were significantly faster, had better agility, and had a greater level of leg strength than Freestyle wrestlers, but Freestyle wrestlers were more flexible than Greco-Roman wrestlers (p < 0.05). Discriminant function analysis indicated that peak arm power, agility, speed, and flexibility were selective factors for the differences between Freestyle and Greco-Roman wrestlers. In conclusion, the present study indicates that the differences between these wrestling styles promote physical fitness differences in elite wrestlers. The results reflect specific features of each wrestling style.

BACKGROUND AND AIMS: Follistatin-like protein-1 (FSTL-1) is considered to be an adipokine or myokine that could be a potential regulator of metabolism. Our purpose is to investigate the relationship between circulating FSTL-1 levels and insulin resistance (IR) in type 2 diabetes mellitus (T2DM) and to identify the regulatory factors. METHODS: FSTL-1 expression in C57BL/6J and db/db mice was examined by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and Western blots. Serum FSTL-1 levels were measured by enzyme-linked immunosorbent assay in 298 T2DM patients and 202 healthy controls. Changes in the circulating FSTL-1 level were observed during the oral glucose tolerance test, EHC (euglycemic-hyperinsulinemic clamp), lipid infusion, acute exercise, and cold-exposure test. RESULTS: We found that FSTL-1 protein expression in the adipose tissue of db/db mice was significantly higher than that of wild-type mice. Importantly, circulating FSTL-1 levels in T2DM and overweight/obese participants were higher than those in healthy and lean individuals, and was related to HOMA-IR, adiponectin, and obesity- and metabolism-related parameters. In the intervention study, 45 minutes of physical activity was found to significantly increase the circulating FSTL-1 concentration in young, healthy participants. Further, FSTL-1 protein expression in adipose tissue rose dramatically in response to physical activity in mice. Hyperinsulinemia during EHC and acute elevated FFA induced by lipid infusion resulted in a significant decrease in the circulating FSTL-1 levels. However, no change was found in the circulating FSTL-1 levels in response to the oral glucose challenge or cold-exposure test. CONCLUSIONS: FSTL-1 may be an adipomyokine associated with insulin resistance and physical activity, and circulating FSTL-1 levels are increased in patients with T2DM.

// Zhi-Gang Gong 1 , Weijie Zhao 2 , Jianbing Zhang 2 , Xi Wu 3 , Jing Hu 3, 4 , Guo-Chang Yin 1 and Yong-Jiang Xu 3, 4 1 Key Laboratory of Training, Monitoring and Intervention of Aquatic Sports of General Administration of Sport of China, Faculty of Physical Education, Jiangxi Normal University, Nanchang, China 2 The First Affiliated Hospital of Sun Yat-sen University, Guangdong, China 3 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China 4 Department of Medicine, University of California San Diego, La Jolla, California, United States Correspondence to: Yong-Jiang Xu, email: yjxutju@gmail.com Keywords: metabolomics, hepatocellular carcinoma, eicosanoid, biomarker, liver cirrhosis Received: February 06, 2017&emsp;&emsp;&emsp;&emsp; Accepted: June 04, 2017&emsp;&emsp;&emsp;&emsp; Published: July 10, 2017 ABSTRACT Hepatocellular carcinoma (HCC) is one of the most common cancers in the world. It is a type of inflammation-related cancer that usually follows liver hepatitis that mostly caused by hepatitis B virus (HBV) in China. However, the metabolism disturbance of HCC and HBV-cirrhosis is not yet fully understood. In addition, there is little research on the relationships between inflammation mediators and HCC. In this study, we investigated serum metabolic abnormalities in HBV-cirrhosis and HCC patients through non-targeted metabolomics and targeted eicosanoid analysis. Metabolomic analysis identified 14 metabolites, i.e . malate, citrate, succinate, lysine, carnitine, proline, ornithine, serine, phenylalanine, tyrosine, arachidonic acid arabinose, galactose and uric acid are consistently altered in HBV-cirrhosis and HCC patients. Meanwhile, eicosanoid analysis uncovered several prostaglandins and leukotrienes are implicated in pathological processes in HBV-cirrhosis and HCC. Finally, these identified biomarkers possessed strong potential to distinguish and diagnose HCC from healthy controls and HBV-cirrhosis patients. This study provided a new perspective to understand the mechanism and discover probable biomarkers of HCC.

As the spectator sport market has become large and competition for consumers has increased, the need for understanding spectators’ motives and points of attachment has become important for developing effective marketing strategies. The purpose of the study was to examine four different models that explain the relationships among motives and points of attachment and determine a model that explains the most variance in the referent variables. A total of 501 college students responded to the Motivation Scale for Sport Consumption (MSSC) and the Points of Attachment Index (PAI). The results showed that motives can be divided into fan motives and spectator motives, and these motives were related to different sets of points of attachment: organizational identification and sport identification.

Nighttime walking is becoming a popular exercise for many middle-aged and older people in Asian countries. However, the health benefits of nighttime walking in urban areas and green spaces are still unclear. This study evaluated the physiological and psychological responses of 48 middle-aged and older people who walked 1.6 km through a green space and an urban area during daytime and nighttime. The Positive and Negative Affect Schedule (PANAS), Profile of Mood States (POMS), Perceived Restorativeness Scale (PRS), and Restorative Outcome Scale (ROS) were employed to measure the psychological responses, and pulse rate and blood pressure (SBP, DBP and MAP) were measured to evaluate the physiological responses. The results showed that the daytime green walking induced psychological improvements and lowered blood pressure (p &lt; 0.05), while the daytime urban walking resulted in slight deterioration of all the measured parameters (p &gt; 0.05). On the other hand, the nighttime green walking induced lowered blood pressure (p &lt; 0.05), whilst the nighttime urban walking resulted in psychological improvements and lowered blood pressure (p &lt; 0.05), and no significant difference was found in any measured parameter between the two nighttime walking groups. In conclusion, urban areas are noisy and irritating in the daytime, and not suitable for walking, but may become pleasurable and attractive at night. The psychological benefits of green walking may decrease at night, and nighttime walking in either an urban area or a green space may achieve similar health benefits. Therefore, we recommend that urban citizens start nighttime walking in a green space or an urban area to keep fit when the air is less polluted.

BACKGROUND: Mainland China has the most smartphone users worldwide, especially among college students, while mindfulness intervention can significantly alleviate the level of problematic smartphone use. We examined the effects of a brief mindfulness intervention on problematic smartphone use and investigated if this effect is mediated by self-control. METHODS: Participants were recruited randomly from a university in Beijing of China. Forty-four college students were assigned to a mindfulness group or a control group. The mindfulness group took part in a brief (30 min) single-session mindfulness intervention. The control group was instructed to listen to a neutral news audio recording for the same duration (30 min). The Freiburg Mindfulness Inventory, Mobile Phone Addiction Tendency Scale, and Self-control Scale were used to measure state mindfulness, problematic smartphone use, and self-control of college students at pre-intervention and post-intervention, respectively. RESULTS: Two-way repeated-measures ANOVA revealed that the mindfulness group had significant improvements in state mindfulness (p = .049) and self-control (p = .012), and had significant alleviation in problematic smartphone use (p < .001) at post-intervention. In the regression model, self-control had a mediating effect between mindfulness intervention and problematic smartphone use (95% CI [0.490, 7.216]). CONCLUSIONS: A brief single-session mindfulness intervention can alleviate the level of problematic smartphone use and increase the level of state mindfulness and self-control compared to the control group. Self-control can completely mediate the efficacy of the mindfulness intervention in reducing problematic smartphone use.

It is established that the physiological effects of insulin are primarily mediated by the insulin signaling pathway. However, a defective insulin signaling is closely associated with the clinical manifestations of polycystic ovary syndrome (PCOS), which include excess androgen levels, insulin resistance and anovulation, and is involved in the pathophysiology of PCOS at the molecular level. Dimethyldiguanide (DMBG) has been widely employed to alleviate reproduction dysfunction in women with PCOS, however, the exact mechanism of this effect remains unclear. The objective of the present study was to investigate the effects of DMBG on the expression of the insulin signaling pathway in the ovaries of rats with PCOS, and to identify the potential underlying molecular mechanisms of these effects in PCOS. In the present study, a PCOS rat model was induced by letrozole, and successful establishment of the model was confirmed by examining ovarian histology and determining serum testosterone levels, by hematoxylin and eosin staining and ELISA, respectively. Subsequently, the expression of two key elements of insulin signaling, insulin receptor substrate (IRS)‑2 and phosphatidylinositol 3‑kinase (PI3K), was determined by immunohistochemistry and western blot analysis. The results demonstrated that IRS‑2 and PI3K expression was markedly decreased in PCOS ovaries, which was rescued by DMBG treatment. These results indicate that IRS‑2/PI3K signaling may be involved in the development of PCOS and the therapeutic effects of DMBG on PCOS. To further confirm the effects of DMBG on insulin signaling expression during this process, the expression of an additional two downstream proteins, phosphoinositide‑dependent kinase‑1 (PDK‑1) and the mammalian target of rapamycin (mTOR), was also investigated in the present study, and the results demonstrated that the expression of PDK‑1 and mTOR was significantly reduced in PCOS ovaries and increased following DMBG treatment, further indicating that altered insulin signaling may have an important role in the development and treatment of PCOS. In conclusion, the results of the present study indicate that the reduced expression of proteins involved in insulin signaling may contribute to the development of the clinical features of PCOS, and DMBG reverses reduced expression of insulin signaling components, by a mechanism that is yet to be determined, to attenuate certain symptoms of PCOS, such as obesity. To the best of our knowledge, the present study is the first to provide data regarding the detailed changes of insulin signaling during the development and treatment of PCOS, and may provide an important reference for clinical PCOS treatment.

This study was to study the impact of aerobic exercises on the chronic unpredictable mild stress (CUMS) in mice, and to discuss the possible mechanism from the skeletal muscle AMPK/PGC-1α energy metabolism signaling pathway. The healthy male mice were randomly divided into Control Group (CG), Model Group (MG), and Model Exercise Group (ME).Twelve stress methods were adopted for four weeks (28 days) to establish the depression model. ME was subject to aerobic training plan after the model was established. The weight of the mice was recorded weekly. After the experimental intervention, the three groups of mice were subjected to behavioral assessment tests. Western blotting, RT-PCR, and ELISA were performed to test AMPK, p-AMPK, PGC-1α, and ATP in skeletal muscle. There were no significant difference in body weight between the three groups. CUMS leaded to significant decline in behavioral scores. and the p-AMPK and PGC-1α decreased significantly. But boosted ATP content. Aerobic exercise enhanced the expressions of p-AMPK and PGC-1α, increased the ratio of p-AMPK/AMPK, boosted ATP content. And improved behavioral scores significantly. Chronic stress-induced depression-like behavior was improved significantly by Aerobic exercise. The mechanism of aerobic exercise for improving depressive symptoms in mice with chronic stress depression may be related to influence AMPK/PGC-1α pathway.