Institute of Mechanical Engineering and Industrial Mangement

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

A review of the investigations that have been made on adhesively bonded joints of fibre-reinforced plastic (FRP) composite structures (single skin and sandwich construction) is presented. The effects of surface preparation, joint configuration, adhesive properties, and environmental factors on the joint behaviour are described briefly for adhesively bonded FRP composite structures. The analytical and numerical methods of stress analysis required before failure prediction are discussed. The numerical approaches cover both linear and non-linear models. Several methods that have been used to predict failure in bonded joints are described. There is no general agreement about the method that should be used to predict failure since the failure strength and modes are different according to the various bonding methods and parameters, but progressive damage models are quite promising since important aspects of the joint behaviour can be modelled by using this approach. However, a lack of reliable failure criteria still exists, limiting in this way a more widespread application of adhesively bonded joints in principal load-bearing structural applications. An accurate strength prediction of the adhesively bonded joints is essential to decrease the amount of expensive testing at the design stage.

This paper presents a review of automated image registration methodologies that have been used in the medical field. The aim of this paper is to be an introduction to the field, provide knowledge on the work that has been developed and to be a suitable reference for those who are looking for registration methods for a specific application. The registration methodologies under review are classified into intensity or feature based. The main steps of these methodologies, the common geometric transformations, the similarity measures and accuracy assessment techniques are introduced and described.

Abstract: The correct modelling of constitutive laws is of critical importance for the analysis of mechanical behaviour of solids and structures. For example, the understanding of soft tissue mechanics, because of the nonlinear behaviour commonly displayed by the mechanical properties of such materials, makes common place the use of hyperelastic constitutive models. Hyperelastic models however, depend on sets of variables that must be obtained experimentally. In this study the authors use a computational/experimental scheme, for the study of the nonlinear mechanical behaviour of biological soft tissues under uniaxial tension. The material constants for seven different hyperelastic material models are obtained via inverse methods. The use of Martins's model to fit experimental data is presented in this paper for the first time. The search for an optimal value for each set of material parameters is performed by a Levenberg–Marquardt algorithm. As a control measure, the process is fully applied to silicone‐rubber samples subjected to uniaxial tension tests. The fitting accuracy of the experimental stress–strain relation to the theoretical one, for both soft tissues and silicone‐rubber (typically nonlinear) is evaluated. This study intents also to select which material models (or model types), the authors will employ in future works, for the analysis of human soft biological tissues.

A new set of six phenomenological failure criteria for fiber-reinforced polymer laminates denoted LaRC03 is described. These criteria can predict matrix and fiber failure accurately, without the curve-fitting parameters. For matrix failure under transverse compression, the angle of the fracture plane is solved by maximizing the Mohr-Coulomb effective stresses. A criterion for fiber kinking is obtained by calculating the fiber misalignment under load and applying the matrix failure criterion in the coordinate frame of the misalignment. Fracture mechanics models of matrix cracks are used to develop a criterion for matrix failure in tension and to calculate the associated in situ strengths. The LaRC03 criteria are applied to a few examples to predict failure load envelopes and to predict the failure mode for each region of the envelope. The analysis results are compared to the predictions using other available failure criteria and with experimental results.

Hydroxyapatite (HA) has been widely used as a biocompatible ceramic in many areas of medicine, but mainly for contact with bone tissue, due to its resemblance to mineral bone. In mammals, the skeleton presents a carbonated and partially substituted apatite, based on nanocrystal aggregates, and associated with collagen, building up 3-D structures present in various bone tissue conformations like trabecular or cancellous bone. There has been growing interest in developing bioactive synthetic ceramics that could closely mimic natural apatite characteristics. This review presents some of the most well known forms of obtaining, by precipitation methods, nanophased HA. Some traditional and more recent developments vis-à-vis the pos-sible HA nanoparticles applications are discussed.

The large surface area and ability to retain moisture of textile structures enable microorganisms' growth, which causes a range of undesirable effects, not only on the textile itself, but also on the user. Due to the public health awareness of the pathogenic effects on personal hygiene and associated health risks, over the last few years, intensive research has been promoted in order to minimize microbes' growth on textiles. Therefore, to impart an antimicrobial ability to textiles, different approaches have been studied, being mainly divided into the inclusion of antimicrobial agents in the textile polymeric fibers or their grafting onto the polymer surface. Regarding the antimicrobial agents, different types have been used, such as quaternary ammonium compounds, triclosan, metal salts, polybiguanides or even natural polymers. Any antimicrobial treatment performed on a textile, besides being efficient against microorganisms, must be non-toxic to the consumer and to the environment. This review mainly intends to provide an overview of antimicrobial agents and treatments that can be performed to produce antimicrobial textiles, using chemical or physical approaches, which are under development or already commercially available in the form of isolated agents or textile fibers or fabrics.

Abstract A layerwise shear deformation theory for composite laminated plates is discretized using multiquadrics. This new type of meshless method considers radial basis functions as the approximation method for both the differential governing equations and the boundary conditions. The combination of this layerwise theory and the multiquadrics discretization method allows a very accurate prediction of the field variables. Laminated composite and sandwich plates are analyzed. The financial support of the Portuguese Foundation for Science and Technology (Fundação para a Ciência e a Tecnologia) under Financiamento Plurianual (POCTI) is gratefully acknowledged.

Abstract Notch features including holes, fillets, shoulders, and grooves commonly exist in engineering components. When subjected to external loads, these geometrical discontinuities generally act as stress raisers and thus present significant influences on the component strength and life, which are more remarkable under complex loading paths. Accordingly, numerous theories and approaches have been developed to address notch effects in metal fatigue as well as damage modelling and life predictions, which aim to provide theoretical support for structural optimal design and integrity evaluation. However, most of them are self‐styled or focus on specific objects, which limits their engineering applicability. This review recalls recent developments and achievements in notch fatigue modelling and analysis of metals. In particular, four commonly used methods for fatigue evaluation of metallic notched components/structures are summarized and elaborated, namely, nominal stress approaches, local stress‐strain approaches, and critical distance theories and weighting control parameters‐based approaches, which intend to provide a reference for further research on notch fatigue analysis and promote the integration and/or development among different approaches for practice.

BACKGROUND: The annual burden of disease caused indoor air pollution, including polluted outdoor air used to ventilate indoor spaces, is estimated to correspond to a loss of over 2 million healthy life years in the European Union (EU). Based on measurements of the European Environment Agency (EEA), approximately 90 % of EU citizens live in areas where the World Health Organization (WHO) guidelines for air quality of particulate matter sized < 2.5 mm (PM2.5) are not met. Since sources of pollution reside in both indoor and outdoor air, selecting the most appropriate ventilation strategy is not a simple and straightforward task. METHODS: A framework for developing European health-based ventilation guidelines was created in 2010-2013 in the EU-funded HEALTHVENT project. As a part of the project, the potential efficiency of control policies to health effects caused by residential indoor exposures of fine particulate matter (PM2.5), outdoor bioaerosols, volatile organic compounds (VOC), carbon oxide (CO) radon and dampness was estimated. The analysis was based on scenario comparison, using an outdoor-indoor mass-balance model and varying the ventilation rates. Health effects were estimated with burden of diseases (BoD) calculations taking into account asthma, cardiovascular (CV) diseases, acute toxication, respiratory infections, lung cancer and chronic obstructive pulmonary disease (COPD). RESULTS: The quantitative comparison of three main policy approaches, (i) optimising ventilation rates only; (ii) filtration of outdoor air; and (iii) indoor source control, showed that all three approaches are able to provide substantial reductions in the health risks, varying from approximately 20 % to 44 %, corresponding to 400 000 and 900 000 saved healthy life years in EU-26. PM2.5 caused majority of the health effects in all included countries, but the importance of the other pollutants varied by country. CONCLUSIONS: The present modelling shows, that combination of controlling the indoor air sources and selecting appropriate ventilation rate was the most effective to reduce health risks. If indoor sources cannot be removed or their emissions cannot be limited to an accepted level, ventilation needs to be increased to remove remaining pollutants. In these cases filtration of outdoor air may be needed to prevent increase of health risks.

The European Foundation for Quality Management (EFQM) 2020 model is a comprehensive and updated business model that encompasses sustainability and shares features with Industry 4.0, emphasizing transformation and improved organizational performance, yet with different theoretical and practical foundations. This research highlights the EFQM 2020 model’s novelties and its relationships/implications with the Industry 4.0 paradigm, contributing to the Quality 4.0 body of knowledge. Several linkages between the EFQM 2020 model and Industry 4.0 have been identified, namely, at the criteria level and guidance points, which can support successful digital transformation by combining quality and excellence with Industry 4.0. However, given the model’s generic and non-prescriptive nature, there is no specific reference to the nine Industry 4.0 pillars. Additionally, the links between direction and organizational culture and leadership criteria and driving performance and transformation are not evident, which might be a concern for business and technology transformation strategies. Managing knowledge, skills, and capabilities is critical for the successful adoption of Industry 4.0. The EFQM model adds a strategic and technologically unbiased perspective to Industry 4.0, providing an integrated business excellence framework for Quality 4.0. With empirical support of the model application, future research is recommended to develop this subject further.

Abstract A grand challenge from the wind energy industry is to provide reliable forecasts on mountain winds several hours in advance at microscale (∼100 m) resolution. This requires better microscale wind-energy physics included in forecasting tools, for which field observations are imperative. While mesoscale (∼1 km) measurements abound, microscale processes are not monitored in practice nor do plentiful measurements exist at this scale. After a decade of preparation, a group of European and U.S. collaborators conducted a field campaign during 1 May–15 June 2017 in Vale Cobrão in central Portugal to delve into microscale processes in complex terrain. This valley is nestled within a parallel double ridge near the town of Perdigão with dominant wind climatology normal to the ridges, offering a nominally simple yet natural setting for fundamental studies. The dense instrument ensemble deployed covered a ∼4 km × 4 km swath horizontally and ∼10 km vertically, with measurement resolutions of tens of meters and seconds. Meteorological data were collected continuously, capturing multiscale flow interactions from synoptic to microscales, diurnal variability, thermal circulation, turbine wake and acoustics, waves, and turbulence. Particularly noteworthy are the extensiveness of the instrument array, space–time scales covered, use of leading-edge multiple-lidar technology alongside conventional tower and remote sensors, fruitful cross-Atlantic partnership, and adaptive management of the campaign. Preliminary data analysis uncovered interesting new phenomena. All data are being archived for public use.

This work presents a review of several investigations on the topic of adhesive bonding at high and low temperatures. Durability and strength at extreme temperatures have always been a major limitation of adhesives that, given their polymeric nature, exhibit substantial degradation at temperatures where other structural materials (such as metals for example) have minute changes in mechanical properties. However, due to the inherent advantages of bonding, there is a large and continued effort aiming to improve the temperature resistance of adhesive joints, and this effort has been spread among the various topics that are discussed in this review. These topics include adhesive shrinkage and thermal expansion, adhesive properties, joint geometry optimization, and design techniques, among others. The findings of these research efforts have all found use in practical applications, helping to solve complex problems in a variety of high-tech industries where there is a constant need to produce light and strong components that can withstand large temperature gradients. Therefore, the final sections of this work include a discussion on two specific application areas that demonstrate the strict demands that extreme temperature use imposes on adhesive joints and the methods used to improve their performance.

Dermoscopy is an imaging technique that has been widely used in the diagnosis of skin lesions. However, its accuracy largely depends on the dermatologist's experience; thus, computer-aided diagnosis techniques are required. In this paper, a novel approach based on a deformable model is proposed to handle the segmentation of skin lesions in dermoscopic images. The RGB color space is converted so that the color information contained in the images can be used effectively to differentiate normal skin and skin lesions; and the differences in the color channels are combined together to define the speed function and the stopping criterion of the deformable model. This novel approach is robust against the noise, and provides an effective and flexible segmentation. Two image databases were used to test the performance of the novel approach and the segmentation results obtained were satisfactory. Quantitative analysis on 250 dermoscopic images showed that the novel algorithm outperformed other state-of-the-art algorithms. Also, using comparative data, the reliability and the implementation issues of the approach are discussed in this paper.

Abstract This paper proposes a new technique which allows the use of simplex finite elements (linear triangles in 2D and linear tetrahedra in 3D) in the large strain analysis of nearly incompressible solids. The new technique extends the F‐bar method proposed by de Souza Neto et al . ( Int. J. Solids and Struct . 1996; 33 : 3277–3296) and is conceptually very simple: It relies on the enforcement of (near‐) incompressibility over a patch of simplex elements (rather than the point‐wise enforcement of conventional displacement‐based finite elements). Within the framework of the F‐bar method, this is achieved by assuming, for each element of a mesh, a modified (F‐bar) deformation gradient whose volumetric component is defined as the volume change ratio of a pre‐defined patch of elements. The resulting constraint relaxation effectively overcomes volumetric locking and allows the successful use of simplex elements under finite strain near‐incompressibility. As the original F‐bar procedure, the present methodology preserves the displacement‐based structure of the finite element equations as well as the strain‐driven format of standard algorithms for numerical integration of path‐dependent constitutive equations and can be used regardless of the constitutive model adopted. The new elements are implemented within an implicit quasi‐static environment. In this context, a closed form expression for the exact tangent stiffness of the new elements is derived. This allows the use of the full Newton–Raphson scheme for equilibrium iterations. The performance of the proposed elements is assessed by means of a comprehensive set of benchmarking two‐ and three‐dimensional numerical examples. Copyright © 2005 John Wiley &amp; Sons, Ltd.

Epoxy resins are one of the most widely used thermosets in different engineering fields, due to their chemical resistance and thermo-mechanical properties. Recently, bio-based thermoset resin systems have attracted significant attention given their environmental benefits related to the wide variety of available natural resources, as well as the resulting reduction in the use of petroleum feedstocks. During the last two decades, considerable improvement on the properties of bio-sourced resins has been achieved to obtain performances comparable to petroleum-based systems. This paper reviews recent advances on new bio-based epoxy resins, derived from natural oils, natural polyphenols, saccharides, natural rubber and rosin. Particular focus has been given to novel chemical formulations and resulting mechanical properties of natural derived- epoxies, curing agents or entire systems, constituting an interesting alternative for a large variety of engineering applications, including the aviation sector. The present work is within the scope of the ECO-COMPASS project, where new bio-sourced epoxy matrixes for green composites are under investigation.

Indoor environmental conditions (thermal, noise, light, and indoor air quality) may affect workers' comfort, and consequently their health and well-being, as well as their productivity. This study aimed to assess the relations between perceived indoor environment and occupants' comfort, and to examine the modifying effects of both personal and building characteristics. Within the framework of the European project OFFICAIR, a questionnaire survey was administered to 7441 workers in 167 "modern" office buildings in eight European countries (Finland, France, Greece, Hungary, Italy, The Netherlands, Portugal, and Spain). Occupants assessed indoor environmental quality (IEQ) using both crude IEQ items (satisfaction with thermal comfort, noise, light, and indoor air quality), and detailed items related to indoor environmental parameters (e.g., too hot/cold temperature, humid/dry air, noise inside/outside, natural/artificial light, odor) of their office environment. Ordinal logistic regression analyses were performed to assess the relations between perceived IEQ and occupants' comfort. The highest association with occupants' overall comfort was found for "noise", followed by "air quality", "light" and "thermal" satisfaction. Analysis of detailed parameters revealed that "noise inside the buildings" was highly associated with occupants' overall comfort. "Layout of the offices" was the next parameter highly associated with overall comfort. The relations between IEQ and comfort differed by personal characteristics (gender, age, and the Effort Reward Imbalance index), and building characteristics (office type and building's location). Workplace design should take into account both occupant and the building characteristics in order to provide healthier and more comfortable conditions to their occupants.

Despite offering very attractive advantages over traditional joining methods, one of the setbacks of adhesive bonding is its long-term strength in aggressive environments, such as environments with high moisture and extreme temperatures. With the rise of new lightweight materials and their recent use in everyday vehicles, transportation industries have been very interested in determining the long-term behavior of adhesive joints. The aim is to build durable, lighter vehicles, which consume less energy and emit less pollution. The two main factors that affect the strength of vehicle adhesive joints are exposure to moist environments and high and low temperatures. There are some works concerning the effect of these two factors separately and some predictive models have been developed, which help the engineer to design reliable, safe, and efficient adhesive joints. However, the combined effect of temperature and moisture is not yet totally understood. This paper presents a review on the temperature and moisture degradation of adhesive joints.

The New European Wind Atlas project will create a freely accessible wind atlas covering Europe and Turkey, develop the model chain to create the atlas and perform a series of experiments on flow in many different kinds of complex terrain to validate the models. This paper describes the experiments of which some are nearly completed while others are in the planning stage. All experiments focus on the flow properties that are relevant for wind turbines, so the main focus is the mean flow and the turbulence at heights between 40 and 300 m. Also extreme winds, wind shear and veer, and diurnal and seasonal variations of the wind are of interest. Common to all the experiments is the use of Doppler lidar systems to supplement and in some cases replace completely meteorological towers. Many of the lidars will be equipped with scan heads that will allow for arbitrary scan patterns by several synchronized systems. Two pilot experiments, one in Portugal and one in Germany, show the value of using multiple synchronized, scanning lidar, both in terms of the accuracy of the measurements and the atmospheric physical processes that can be studied. The experimental data will be used for validation of atmospheric flow models and will by the end of the project be freely available.This article is part of the themed issue 'Wind energy in complex terrains'.

In the European research project OFFICAIR, a procedure was developed to determine associations between characteristics of European offices and health and comfort of office workers, through a checklist and a self-administered questionnaire including environmental, physiological, psychological, and social aspects. This procedure was applied in 167 office buildings in eight European countries (Portugal, Spain, Italy, Greece, France, Hungary, the Netherlands, and Finland) during the winter of 2011-2012. About 26 735 survey invitation e-mails were sent, and 7441 office workers were included in the survey. Among respondents who rated an overall comfort less than 4 (23%), 'noise (other than from building systems)', air 'too dry', and temperature 'too variable' were the main complaints selected. An increase of perceived control over indoor climate was positively associated with the perceived indoor environment quality. Almost one-third of office workers suffered from dry eyes and headache in the last 4 weeks. Physical building characteristics were associated with occupants' overall satisfaction (acoustical solutions, mold growth, complaints procedure, cleaning activities) and health (number of occupants, lack of operable windows, presence of carpet and cleaning activities). OFFICAIR project provides a useful database to identify stressors related to indoor environmental quality and office worker's health.