Institute of Physics

We present what is to our knowledge the first in vivo tomograms of human retina obtained by Fourier domain optical coherence tomography. We would like to show that this technique might be as powerful as other optical coherence tomography techniques in the ophthalmologic imaging field. The method, experimental setup, data processing, and images are discussed.

CERN-LHC. Measurement of inclusive charged particle distributions in proton proton collisions at centre-of-mass energies 0.9, 2.36 and 7 TeV using a single-arm minimum-bias trigger. Distributions of charged particle multiplicity and its dependence on pseudorapidity and transverse momentum are presented from ~190 mub-1 of data at 7 Tev, ~7 mub-1 at 0.9 TeV, and ~0.1 mub-1 at 2.36 GeV. UPDATE 20/05/2016: A wrong point was removed from table 16.

We interfaced color Doppler Fourier domain optical coherence tomography (CD-FDOCT) with a commercial OCT system to perform in vivo studies of human retinal blood flow in real time. FDOCT does not need reference arm scanning and records one full depth and Doppler profile in parallel. The system operates with an equivalent A-scan rate of 25 kHz and allows real time imaging of the color encoded Doppler information together with the tissue morphology at a rate of 2-4 tomograms (40 x 512 pixel) per second. The recording time of a single tomogram (160 x 512 data points) is only 6,4ms. Despite the high detection speed we achieve a system sensitivity of 86dB using a beam power of 500microW at the cornea. The fundus camera allows simultaneous view for selection of the region of interest. We observe bi-directional blood flow and pulsatility of blood velocity in retinal vessels with a Doppler detection bandwidth of 12.5 kHz and a longitudinal velocity sensitivity in tissue of 200microm/s.

Relativistic corrections, Born–Oppenheimer energies and adiabatic corrections are computed for R ≤ 12.0 bohr for the electronic ground state of the hydrogen molecule. The Born–Oppenheimer potential is slightly lower than ever reported. The problem of linear dependencies in the basis set is removed and the same set is used for all internuclear distances which assures continuity of the results. The radiative corrections are evaluated approximately and—for that purpose—the polarizability of the molecule is also computed. Vibrational energies are computed and— corrected for nonadiabatic effects—compared with experiment for several isotopes. It is argued on the basis of the remaining discrepancies that an improvement in the ab initio nonadiabatic corrections is necessary.

With over 30,000 confirmed cases, Brazil is currently the country most affected by COVID-19 in Latin America, and ranked 12th worldwide (John Hopkins University & Medicine, 2020). Despite all evidence, a strong rhetoric undermining risks associated to COVID-19 has been endorsed at the highest levels of the Brazilian government, making President Jair Bolsonaro the leader of the “coronavirus-denial movement” (Friedman, 2020. To support this strategy, different forms of misinformation and disinformation2 have been leveraged to lead a dangerous crusade against scientific and evidence-based recommendations (Ireton & Posetti, 2018).

Abstract Background Metastasis causes the most breast cancer-related deaths in women. Here, we investigated the antitumor effect of solid lipid nanoparticles (SLN-DTX) when used in the treatment of metastatic breast tumors using 4T1-bearing BALB/c mice. Results Solid lipid nanoparticles (SLNs) were produced using the high-energy method. Compritol 888 ATO was selected as the lipid matrix, and Pluronic F127 and Span 80 as the surfactants to stabilize nanoparticle dispersion. The particles had high stability for at least 120 days. The SLNs’ dispersion size was 128 nm, their polydispersity index (PDI) was 0.2, and they showed a negative zeta potential. SLNs had high docetaxel (DTX) entrapment efficiency (86%), 2% of drug loading and showed a controlled drug-release profile. The half-maximal inhibitory concentration (IC 50 ) of SLN-DTX against 4T1 cells was more than 100 times lower than that of free DTX after 24 h treatment. In the cellular uptake test, SLN-DTX was taken into the cells significantly more than free DTX. The accumulation in the G2-M phase was significantly higher in cells treated with SLN-DTX (73.7%) than in cells treated with free DTX (23.0%), which induced subsequent apoptosis. TEM analysis revealed that SLN-DTX internalization is mediated by endocytosis, and fluorescence microscopy showed DTX induced microtubule damage. In vivo studies showed that SLN-DTX compared to free docetaxel exhibited higher antitumor efficacy by reducing tumor volume (p < 0.0001) and also prevented spontaneous lung metastasis in 4T1 tumor-bearing mice. Histological studies of lungs confirmed that treatment with SLN-DTX was able to prevent tumor. IL-6 serum levels, ki-67 and BCL-2 expression were analyzed and showed a remarkably strong reduction when used in a combined treatment. Conclusions These results indicate that DTX-loaded SLNs may be a promising carrier to treat breast cancer and in metastasis prevention.

Travelers today use technology that generates vast amounts of data at low cost. These data could supplement most outputs of regional travel demand models. New analysis tools could change how data and modeling are used in the assessment of travel demand. Recent work has shown how processed origin–destination trips, as developed by trip data providers, support travel analysis. Much less has been reported on how raw data from telecommunication providers can be processed to support such an analysis or to what extent the raw data can be treated to extract travel behavior. This paper discusses how cell phone data can be processed to inform a four-step transportation model, with a focus on the limitations and opportunities of such data. The illustrated data treatment approach uses only phone data and population density to generate trip matrices in two metropolitan areas: Boston, Massachusetts, and Rio de Janeiro, Brazil. How to label zones as home- and work-based according to frequency and time of day is detailed. By using the labels (home, work, or other) of consecutive stays, one can assign purposes to trips such as home-based work. The resulting trip pairs are expanded for the total population from census data. Comparable results with existing information reported in local surveys in Boston and existing origin–destination matrices in Rio de Janeiro are shown. The results detail a method for use of passively generated cellular data as a low-cost option for transportation planning.

BACKGROUND: Ethylene is a phytohormone known for inducing a triple response in seedlings, leaf abscission and other responses to various stresses. Several studies in model plants have evaluated the importance of this hormone in crosstalk signaling with different metabolic pathways, in addition to responses to biotic stresses. However, the mechanism of action in plants of agricultural interest, such as soybean, and its participation in abiotic stresses remain unclear. RESULTS: The studies presented in this work allowed for the identification of 176 soybean genes described elsewhere for ethylene biosynthesis (108 genes) and signal transduction (68 genes). A model to predict these routes in soybean was proposed, and it had great representability compared to those described for Arabidopsis thaliana and Oryza sativa. Furthermore, analysis of putative gene promoters from soybean gene orthologs permitted the identification of 29 families of cis-acting elements. These elements are essential for ethylene-mediated regulation and its possible crosstalk with other signaling pathways mediated by other plant hormones. From genes that are differentially expressed in the transcriptome database, we analyzed the relative expression of some selected genes in resistant and tolerant soybean plants subjected to water deficit. The differential expression of a set of five soybean ethylene-related genes (MAT, ACS, ACO, ETR and CTR) was validated with RT-qPCR experiments, which confirmed variations in the expression of these soybean target genes, as identified in the transcriptome database. In particular, two families of ethylene biosynthesis genes (ACS and ACO) were upregulated under these experimental conditions, whereas CTR (involved in ethylene signal transduction) was downregulated. In the same samples, high levels of ethylene production were detected and were directly correlated with the free fraction levels of ethylene's precursor. Thus, the combination of these data indicated the involvement of ethylene biosynthesis and signaling in soybean responses to water stress. CONCLUSIONS: The in silico analysis, combined with the quantification of ethylene production (and its precursor) and RT-qPCR experiments, allowed for a better understanding of the importance of ethylene at a molecular level in this crop as well as its role in the response to abiotic stresses. In summary, all of the data presented here suggested that soybean responses to water stress could be regulated by a crosstalk network among different signaling pathways, which might involve various phytohormones, such as auxins, ABA and jasmonic acid. The integration of in silico and physiological data could also contribute to the application of biotechnological strategies to the development of improved cultivars with regard to different stresses, such as the isolation of stress-specific plant promoters.

The toxicity of cancer chemotherapy is among the most important factors limiting its use. Clear delineation and communication of benefits and risks is an essential component of treatment decisions. Gastrointestinal toxicity during chemotherapy is frequent and contributes to dose reductions, delays and cessation of cancer treatment. The development of intervention strategies that could eliminate an expected side effect of chemotherapy is vital. Physiologic changes that can increase the toxicity of chemotherapy are decreased stem cell reserves, decreased ability to repair cell damage, progressive loss of body protein, and accumulation of body fat. Symptoms only arise when physiological functions are altered. The gastrointestinal symptoms arising during cancer chemotherapy can often be cured if newly acquired, and if gastrointestinal physiological deficits are identified. Developing new chemotherapy regimens with similar efficacy but less toxicity should be a priority for future research.

Abstract: The African continent hosts some of the largest freshwater systems worldwide, characterized by a large distribution and variability of surface waters that play a key role in the water, energy and carbon cycles and are of major importance to the global climate and water resources. Freshwater availability in Africa has now become of major concern under the combined effect of climate change, environmental alterations and anthropogenic pressure. However, the hydrology of the African river basins remains one of the least studied worldwide and a better monitoring and understanding of the hydrological processes across the continent become fundamental. Earth Observation, that offers a cost-effective means for monitoring the terrestrial water cycle, plays a major role in supporting surface hydrology investigations. Remote sensing advances are therefore a game changer to develop comprehensive observing systems to monitor Africa's land water and manage its water resources. Here, we review the achievements of more than three decades of advances using remote sensing to study surface waters in Africa, highlighting the current benefits and difficulties. We show how the availability of a large number of sensors and observations, coupled with models, offers new possibilities to monitor a continent with scarce gauged stations. In the context of upcoming satellite missions dedicated to surface hydrology, such as the Surface Water and Ocean Topography (SWOT), we discuss future opportunities and how the use of remote sensing could benefit scientific and societal applications, such as water resource management, flood risk prevention and environment monitoring under current global change. Article Highlights: The hydrology of African surface water is of global importance, yet it remains poorly monitored and understoodComprehensive review of remote sensing and modeling advances to monitor Africa's surface water and water resourcesFuture opportunities with upcoming satellite missions and to translate scientific advances into societal applications.

We present an overview of the current state of the literature and research performed by the authors of the present paper on the experimental and theoretical results on the structural-, optical-, nonlinear optical (NLO)-properties (including two-photon absorption (TPA) and the terahertz (THz) range of spectra) and practical applications of a highly anisotropic Gallium Selenide (GaSe) semiconductor with emphasis on the ɛ-GaSe. Physical properties of ɛ-GaSe are important to researchers and designers developing different devices by using this material. This crystal possesses an outstanding NLO properties: high optical birefringence Δn ∼ 0.3 at 700 nm; high transparency range (0.7−18.0 μm) with low absorption coefficient (α ≤ 0.3 cm−1); very high nonlinear susceptibility χ(2) (d 22 ≈ 86 ± 17 pm/V, corresponding to (2.0 ± 0.4) × 10−7 esu) that is used for phase matched second harmonic generation (SHG) in a wide transparency range; high power threshold for optical damage; possibility to perform optical frequency conversion under phase-matching conditions in the near- to mid-IR and THz range of spectra, etc. The domain structure of crystal in connection with the NLO properties is discussed as studied by confocal Raman microscopy experiments. Perspectives for future research of GaSe are considered in the present article, which does not pretend to be one reflecting all existing papers on GaSe crystal and discussed subjects.

Recent paleoclimatic studies suggest that changes in the tropical rainbelt across the Atlantic Ocean during the past two millennia are linked to a latitudinal shift of the Intertropical Convergence Zone (ITCZ) driven by the Northern Hemisphere (NH) climate. However, little is known regarding other potential drivers that can affect tropical Atlantic rainfall, mainly due to the scarcity of adequate and high-resolution records. In this study, we fill this gap by reconstructing precipitation changes in Northeastern Brazil during the last 2,300 years from a high-resolution lake record of hydrogen isotope compositions of plant waxes. We find that regional precipitation along the coastal area of South America was not solely governed by north-south displacements of the ITCZ due to changes in NH climate, but also by the contraction and expansion of the tropical rainbelt due to variations in sea surface temperature and southeast trade winds in the tropical South Atlantic Basin.

BACKGROUND: In the photodynamic therapy (PDT), the photosensitizer absorbs light and transfers the energy of the excited state to the oxygen in the cell environment producing reactive oxygen species (ROS), that in its turn, may cause cell damage. In the photothermal therapy (PTT), light also is responsible for activating the photothermal agent, which converts the absorbed energy in heat. Graphene oxide is a carbon-based material that presents photothermal activity. Its physical properties allow the association with the photosensitizer methylene blue and consequently the production of ROS when submitted to light irradiation. Therefore, the association between nanographene oxide and methylene blue could represent a strategy to enhance therapeutic actions. In this work, we report the nanographene oxide-methylene blue platform (NanoGO-MB) used to promote tumor ablation in combination with photodynamic and photothermal therapies against a syngeneic orthotopic murine breast cancer model. RESULTS: In vitro, NanoGO-MB presented 50% of the reactive oxygen species production compared to the free MB after LED light irradiation, and a temperature increase of ~ 40 °C followed by laser irradiation. On cells, the ROS production by the nanoplatform displayed higher values in tumor than normal cells. In vivo assays demonstrated a synergistic effect obtained by the combined PDT/PTT therapies using NanoGO-MB, which promoted complete tumor ablation in 5/5 animals. Up to 30 days after the last treatment, there was no tumor regrowth compared with only PDT or PTT groups, which displayed tumoral bioluminescence 63-fold higher than the combined treatment group. Histological studies confirmed that the combined therapies were able to prevent tumor regrowth and liver, lung and spleen metastasis. In addition, low systemic toxicity was observed in pathologic examinations of liver, spleen, lungs, and kidneys. CONCLUSIONS: The treatment with combined PDT/PTT therapies using NanoGO-MB induced more toxicity on breast carcinoma cells than on normal cells. In vivo, the combined therapies promoted complete tumor ablation and metastasis prevention while only PDT or PTT were unable to stop tumor development. The results show the potential of NanoGO-MB in combination with the phototherapies in the treatment of the breast cancer and metastasis prevention.

Abstract. Carbon cycling in the Amazon is closely linked to atmospheric processes and climate in the region as a consequence of the strong coupling between the atmosphere and biosphere. This work examines the effects of changes in net radiation due to atmospheric aerosol particles and clouds on the net ecosystem exchange (NEE) of CO2 in the Amazon region. Some of the major environmental factors affecting the photosynthetic activity of plants, such as air temperature and relative humidity, were also examined. An algorithm for clear-sky irradiance was developed and used to determine the relative irradiance, f, which quantifies the percentage of solar radiation absorbed and scattered due to atmospheric aerosol particles and clouds. Aerosol optical depth (AOD) was calculated from irradiances measured with the MODIS (Moderate Resolution Imaging Spectroradiometer) sensor, onboard the Terra and Aqua satellites, and was validated with ground-based AOD measurements from AERONET (Aerosol Robotic Network) sun photometers. Carbon fluxes were measured using eddy covariance technique at the Large-Scale Biosphere-Atmosphere Experiment in Amazonia (LBA) flux towers. Two sites were studied: the Jaru Biological Reserve (RBJ), located in Rondonia, and the Cuieiras Biological Reserve at the K34 LBA tower (located in a preserved region in the central Amazon). Analysis was performed continuously from 1999 to 2009 at K34 and from 1999 to 2002 at RBJ, and includes wet, dry and transition seasons. In the Jaru Biological Reserve, a 29% increase in carbon uptake (NEE) was observed when the AOD ranged from 0.10 to 1.5 at 550 nm. In the Cuieiras Biological Reserve, the aerosol effect on NEE was smaller, accounting for an approximate 20% increase in NEE. High aerosol loading (AOD above 3 at 550 nm) or high cloud cover leads to reductions in solar flux and strong decreases in photosynthesis up to the point where NEE approaches zero. The observed increase in NEE is attributed to an enhancement (~50%) in the diffuse fraction of photosynthetic active radiation (PAR). The enhancement in diffuse PAR can be done through increases in aerosols and/or clouds. In the present study, it was not possible to separate these two components. Significant changes in air temperature and relative humidity resulting from changes in solar radiation fluxes under high aerosol loading were also observed at both sites. Considering the long-range transport of aerosols in the Amazon, the observed changes in NEE for these two sites may occur over large areas in the Amazon, significantly altering the carbon balance in the largest rainforest in the world.

Hydroperoxy-eicosatetraenoyl-phosphatidylethanolamine (HpETE-PE) is a ferroptotic cell death signal. HpETE-PE is produced by the 15-Lipoxygenase (15LOX)/Phosphatidylethanolamine Binding Protein-1 (PEBP1) complex or via an Fe-catalyzed non-enzymatic radical reaction. Ferrostatin-1 (Fer-1), a common ferroptosis inhibitor, is a lipophilic radical scavenger but a poor 15LOX inhibitor arguing against 15LOX having a role in ferroptosis. In the current work, we demonstrate that Fer-1 does not affect 15LOX alone, however, it effectively inhibits HpETE-PE production by the 15LOX/PEBP1 complex. Computational molecular modeling shows that Fer-1 binds to the 15LOX/PEBP1 complex at three sites and could disrupt the catalytically required allosteric motions of the 15LOX/PEBP1 complex. Using nine ferroptosis cell/tissue models, we show that HpETE-PE is produced by the 15LOX/PEBP1 complex and resolve the long-existing Fer-1 anti-ferroptotic paradox.

The electronic structure of two-dimensional gallium selenide crystals containing a small number of layers was investigated theoretically and experimentally. The electronic band structure of the layered GaSe crystal was investigated by the first-principles density functional theory calculations. The $GW$ approximation was used for the correction of the band-gap values. A dependence of the band-gap value on the number of tetralayers has been demonstrated. For the thin crystal with several tetralayers, the band gap becomes larger compared to the bulk crystal. The thin layers of GaSe have been experimentally produced by the ultrasonication of GaSe particles in water suspensions in the presence of Pluronic F127 surfactant. Their thickness was from one to a few tetralayers, according to the transmission electron microscopy studies. The optical absorption spectra demonstrate the well-resolved bands, shifted toward the blue relative to those of the bulk GaSe. Their origin is caused by the presence of GaSe structures with one to a few tetralayers.

Abstract Two-dimensional lead halide perovskites have demonstrated their potential as high-performance scintillators for X- and gamma-ray detection, while also being low-cost. Here we adopt lithium chemical doping in two-dimensional phenethylammonium lead bromide (PEA) 2 PbBr 4 perovskite crystals to improve the properties and add functionalities with other radiation detections. Li doping is confirmed by X-ray photoemission spectroscopy and the scintillation mechanisms are explored via temperature dependent X-ray and thermoluminescence measurements. Our 1:1 Li-doped (PEA) 2 PbBr 4 demonstrates a fast decay time of 11 ns (80%), a clear photopeak with an energy resolution of 12.4%, and a scintillation yield of 11,000 photons per MeV under 662 keV gamma-ray radiation. Additionally, our Li-doped crystal shows a clear alpha particle/gamma-ray discrimination and promising thermal neutron detection through 6 Li enrichment. X-ray imaging pictures with (PEA) 2 PbBr 4 are also presented. All results demonstrate the potential of Li-doped (PEA) 2 PbBr 4 as a versatile scintillator covering a wide radiation energy range for various applications.

Iron oxides that exhibit magnetic properties have been widely studied not only from an academic standpoint, but also for numerous applications in different fields of knowledge, such as biomedical and technological research. In this work, magnetite and maghemite nanoparticles were synthesized by chemical coprecipitation of FeCl 2 ·4H 2 O and FeCl 3 ·6H 2 O (proportion of 1 : 2) in three different cases using two bases (sodium hydroxide and hydroxide ammonium) as precipitants. The chemical coprecipitation method was selected for its simplicity, convenience, reproducibility, and low cost in the use of glassware. The nanostructured materials were characterized by transmission electron microscopy (TEM), X‐ray diffraction (XRD) and magnetometry (VSM). The objective of this work is to study the variation in the morphological characteristics and physical properties of nanoparticles magnetic as a function of the different production processes. As observed by TEM, the materials obtained from the precipitating agent NH 4 OH are more uniform than those obtained with NaOH. From XRD pattern analysis, it appears that the obtained materials correspond to magnetite and maghemite and, from magnetometry VSM analysis, show high magnetization as a function of the magnetic field at room temperature, indicating that these materials are superparamagnetic.

Abstract The mechanism of a field induced transition from helical Sm C∗ to Sm C structure in a DOBAMBC liquid crystal situated between two glass plates has been studied by optical methods. The important role of a regular system of twist disclination lines (dechiralization lines) in unwinding the helical structure by electric field has been revealed. A simplified model of ±2π twist disclination pairs is proposed which describes the unwinding of the Sm C∗ structure. Under the external electric field the pairs of ±2π twist disclinations move and annihilate each other and thus effectively unwind the helical structure of the sample. Critical fields for switching the helical Sm C∗ structure, as well as the unwound structure, are estimated.

Among primates, only chimpanzees and orang-utans are credited with customary tool use in nature. Among monkeys, capuchins stand out with respect to the number of accounts of tool use. However, the majority of capuchin tool use observations reported in nature is anecdotal or idiosyncratic. In this report, we documented the stone pounding of dry fruits (Hymenea courbaril and Acrocomia aculeata) in two wild free-ranging groups of Cebus libidinosus in the Brasilia National Park, a preserved area representative of the Cerradobiome of Central Brazil. In 2004, we noted 2 episodes at which 4 monkeys used stones to crack open nuts. In 2005, we recorded 5 pounding episodes involving 2 different monkeys. Observations of tool use over the course of 2 consecutive years by some individuals, as well as other indirect evidence, indicate that this behaviour could be habitual in the studied groups. We propose that the probability of the emergence of the use of pounding stones as tools may be dependent on the ecological variables that influence the degree of terrestriality and extractive foraging and the complex interaction of these factors.