Instituto Superior de Engenharia do Porto

Disposable sensors are low-cost and easy-to-use sensing devices intended for short-term or rapid single-point measurements. The growing demand for fast, accessible, and reliable information in a vastly connected world makes disposable sensors increasingly important. The areas of application for such devices are numerous, ranging from pharmaceutical, agricultural, environmental, forensic, and food sciences to wearables and clinical diagnostics, especially in resource-limited settings. The capabilities of disposable sensors can extend beyond measuring traditional physical quantities (for example, temperature or pressure); they can provide critical chemical and biological information (chemo- and biosensors) that can be digitized and made available to users and centralized/decentralized facilities for data storage, remotely. These features could pave the way for new classes of low-cost systems for health, food, and environmental monitoring that can democratize sensing across the globe. Here, a brief insight into the materials and basics of sensors (methods of transduction, molecular recognition, and amplification) is provided followed by a comprehensive and critical overview of the disposable sensors currently used for medical diagnostics, food, and environmental analysis. Finally, views on how the field of disposable sensing devices will continue its evolution are discussed, including the future trends, challenges, and opportunities.

Optimisation problems in Healthcare have received considerable attention for more than three decades. More recently, however, with decreasing birth rates in nearly all of the developed countries and increasing average longevity globally, optimisation issues in Healthcare have become noticeably important and attract keen interest from the Operations Research community. Over the years, attention has gradually expanded from resource allocation and strategic planning to include operational issues such as resource scheduling and treatment planning. This paper surveys several applications of Operations Research in the domain of Healthcare. In particular, the paper reviews key contributions addressing contemporary optimisation issues in this area. It highlights current research activities, focusing on a variety of optimisation problems as well as solution techniques used for solving the optimisation problems.

Metals occur naturally in the earth's crust, and their contents in the environment can vary between different regions resulting in spatial variations of background concentrations. The distribution of metals in the environment is governed by the properties of the metal and influences of environmental factors (Khlifi & Hamza-Chaffai, 2010). Of the 92 naturally

The early evolution of SW Europe Variscides started by opening of the Rheic ocean at ∼500 Ma, splitting Avalonia from Armorica/Iberia. Subduction on the SE side of Rheic generated the Paleotethys back‐arc basin (430–390 Ma, splitting Armorica from Iberia), with development of Porto‐Tomar‐Ferreira do Alentejo (PTFA) dextral transform defining the boundary between continental Armorica and Finisterra microplate to the W. Obduction of Paleotethys was followed by Armorica/Iberia collision and emplacement of NW Iberian Allochthonous Units at 390–370 Ma, whereas toward the west of PTFA, there was antithetic ophiolite obduction (Beja‐Acebuches and Rheic ophiolites plus Finisterra continental slices) on top of Ossa‐Morena Zone, with simultaneous development of eclogites and orogenic magmatism under a flake–double wedge tectonic regime. Continued convergence (<370 Ma) proceeded by intracontinental deformation, with progressive tightening of the Ibero‐Armorican Arc through dextral transpression on the Cantabrian Indentor, from Iberia to Armorica. The proposed model is discussed at the light of the driving mechanism of “soft plate tectonics.”

The occurrence and fate of pharmaceuticals in the aquatic environment is recognized as one of the emerging issues in environmental chemistry. Conventional wastewater treatment plants (WWTPs) are not designed to remove pharmaceuticals (and their metabolites) from domestic wastewaters. The treatability of pharmaceutical compounds in WWTPs varies considerably depending on the type of compound since their biodegradability can differ significantly. As a consequence, they may reach the aquatic environment, directly or by leaching of the sludge produced by these facilities. Currently, the technologies under research for the removal of pharmaceuticals, namely membrane technologies and advanced oxidation processes, have high operation costs related to energy and chemical consumption. When chemical reactions are involved, other aspects to consider include the formation of harmful reaction by-products and the management of the toxic sludge produced. Research is needed in order to develop economic and sustainable treatment processes, such as bioremediation and biosorption. The use of low-cost materials, such as biological matrices (e.g., algae and fungi), has advantages such as low capital investment, easy operation, low operation costs, and the non-formation of degradation by-products. An extensive review of existing research on this subject is presented.

Abstract The available techniques for strength prediction of bonded joints have improved over the years. Cohesive zone models (CZM) coupled to finite element method (FEM) analyses surpass the limitations of stress/strain and fracture criteria, and simulate damage growth. CZMs require the instantaneous energy release rates in tension (G n) and shear (G s) along the fracture paths and respective fracture energies in tension (G n c) and shear (G s c), and crack growth is ruled by traction-separation laws that are established at the failure paths. Additionally, the cohesive strengths must be defined (t n 0 for tension and t s 0 for shear) relating to the onset of damage. A few techniques are available for the estimation of these parameters (e.g., the property identification technique, the direct method and the inverse method) that differ in complexity and expected accuracy of the results. In this work, the influence of the cohesive law parameters of a triangular CZM used to model a thin adhesive layer in bonded joints is studied, to estimate their effect on the predictions. Some conclusions were established to provide important data for the proper selection of the estimation technique and expected accuracy of the simulation results. KEYWORDS: Bonded jointsCohesive zone modelsFinite element methodStructural adhesive Notes Presented in part at the 1st International Conference on Structural Adhesive Bonding (AB2011), Porto, Portugal, 7–8 July 2011.

For the predicted growth of e-commerce, supply chains need to adapt to new conditions, so that delivery can be fast, cheap and reliable. The key to success is the last-mile product delivery (LMD) – the last stage of the supply chain, where the ordered product is delivered to the final consumer’s location. One innovative proposal puts foundations in a new delivery model where a professional delivery fleet (PF) is supplemented partially or fully with crowdshipping. The main idea of crowdshipping is to involve ordinary people – in our case in-store shoppers – in the delivery of packages to other customers. In return, occasional couriers (OC) are offered a small compensation. In hitherto formulated problems it was assumed that OCs always accept delivery tasks assigned to them. In this paper we consider OCs as independent agents, which are free to reject assignments. The main contribution of the paper is an original bi-level methodology for matching and routing problem in LMD with OCs and the PF. The goal is to use crowdshipping to reduce the total delivery cost in a same-day last-mile delivery system with respect to occasional couriers’ freedom to accept or reject the assigned delivery. We introduce probability to represent each OC’s willingness to perform the delivery to a given final customer. We study the OCs’ willingness to accept or reject delivery tasks assigned to them and the influence of their decision on the total delivery cost associated to both the OCs’ compensation fees and the delivery cost generated by the PF used for the delivery of remaining parcels.

QuEChERS method was evaluated for extraction of 16 PAHs from fish samples. For a selective measurement of the compounds, extracts were analysed by LC with fluorescence detection. The overall analytical procedure was validated by systematic recovery experiments at three levels and by using the standard reference material SRM 2977 (mussel tissue). The targeted contaminants, except naphthalene and acenaphthene, were successfully extracted from SRM 2977 with recoveries ranging from 63.5-110.0% with variation coefficients not exceeding 8%. The optimum QuEChERS conditions were the following: 5 g of homogenised fish sample, 10 mL of ACN, agitation performed by vortex during 3 min. Quantification limits ranging from 0.12-1.90 ng/g wet weight (0.30-4.70 microg/L) were obtained. The optimized methodology was applied to assess the safety concerning PAHs contents of horse mackerel (Trachurus trachurus), chub mackerel (Scomber japonicus), sardine (Sardina pilchardus) and farmed seabass (Dicentrarchus labrax). Although benzo(a)pyrene, the marker used for evaluating the carcinogenic risk of PAHs in food, was not detected in the analysed samples (89 individuals corresponding to 27 homogenized samples), the overall mean concentration ranged from 2.52 +/- 1.20 ng/g in horse mackerel to 14.6 +/- 2.8 ng/g in farmed seabass. Significant differences were found between the mean PAHs concentrations of the four groups.

Adhesive bonding of components has become more efficient in recent years due to the developments in adhesive technology, which has resulted in higher peel and shear strengths, and also in allowable ductility up to failure. As a result, fastening and riveting methods are being progressively replaced by adhesive bonding, allowing a big step towards stronger and lighter unions. However, single-lap bonded joints still generate substantial peel and shear stress concentrations at the overlap edges that can be harmful to the structure, especially when using brittle adhesives that do not allow plasticization in these regions. In this work, a numerical and experimental study is performed to evaluate the feasibility of bending the adherends at the ends of the overlap for the strength improvement of single-lap aluminium joints bonded with a brittle and a ductile adhesive. Different combinations of joint eccentricity were tested, including absence of eccentricity, allowing the optimization of the joint. A Finite Element stress and failure analysis in ABAQUS® was also carried out to provide a better understanding of the bent configuration. Results showed a major advantage of using the proposed modification for the brittle adhesive, but the joints with the ductile adhesive were not much affected by the bending technique.

This work presents an alternative solution for simultaneous measurement of strain and temperature. The sensing head is formed by a long-period fiber grating combined with a high-birefringence fiber loop mirror resulting in a configuration capable of temperature and strain discrimination. These optical devices have opposite sensitivity responses when a variation of temperature and/or strain is applied. Maximum errors of plusmn0.8degC and plusmn21muepsiv are reported over 60 degC and 700-muepsiv measurement ranges, respectively

We propose a wireless medium access control (MAC) protocol that provides static-priority scheduling of messages in a guaranteed collision-free manner. Our protocol supports multiple broadcast domains, resolves the wireless hidden node problem and allows for parallel transmissions across a mesh network. Arbitration of messages is achieved without the notion of a master coordinating node, global clock synchronization or out-ofband signalling. The protocol relies on bit-dominance similar to what is used in the CAN bus except that in order to operate on a wireless physical layer, nodes are not required to receive incoming bits while transmitting. The use of bit-dominance efficiently allows for a much larger number of priorities than would be possible using existing wireless solutions. A MAC protocol with these properties enables schedulability analysis of sporadic message streams in wireless multihop networks.

Abstract This study describes stress and failure analyses of tensile loaded repaired Carbon Fibre Reinforced Composite (CFRP) laminates, using scarf configuration. A numerical model including interface finite elements was used to obtain peel and shear-stress distributions in the directions tangent and normal to the scarf. These stresses were evaluated at several locations in the repair, namely in the middle of the adhesive, at interfaces between adhesive and patch, and between adhesive and parent material. Several scarf angle values were considered in the analysis. A cohesive mixed-mode damage model was also used to carry out the failure analysis, in order to assess the efficiency of the repairs, for different stacking sequences. A study was performed to evaluate the influence of the mechanical properties of the adhesive and parent laminate/adhesive and adhesive/patch interfaces on the strength and failure modes of the joint. It was concluded that the strengths of the adhesive and interfaces are more important than the fracture properties in the failure process of the repair. It was also verified that the strength of the repair increased exponentially with the scarf angle reduction. Keywords: ADHESIVE JOINTSSTRENGTHLAMINATESFINITE ELEMENT ANALYSISREPAIR

The esterification of hydrophilic phenolic antioxidants is an efficient approach to enhance their solubility in apolar media. Herein, structure-property studies on the antiradical activity of a series of protocatechuic acid alkyl esters have been accomplished. The increase of the lipophilicity was shown to significantly improve the antioxidant activity of protocatechuic esters. Their efficiency as radical scavengers was evaluated using distinctive analytical methods, namely, 2,2-diphenyl-1-picrylhydrazyl (DPPH) UV/visible method, electrochemistry, and differential scanning calorimetry. All the new alkyl protocatechuate antioxidants studied possessed better radical-scavenging capacity than the natural antioxidant protocatechuic acid. This work has shown that the alkyl ester side chain markedly influences the lipophilicity of this type of phenolic system without disturbing the core of the molecule responsible for antioxidant activity. The data on the antioxidant activity obtained using the different analytical methods correlated well with each other and have revealed the interesting antioxidant potential of alkyl esters of protocatechuic acid.

fruits occupy an important position in the context of the fruit trade, considering that both fresh fruits and processed products are produced on a large scale. Citrus fruits are recognized as an essential component of the human diet, thanks to their high content of beneficial nutrients such as vitamins, minerals, terpenes, flavonoids, coumarins and dietary fibers. Among these, a wide range of positive biological activities are attributed to terpenes and flavonoids derivatives. In this review, a list of bibliographic reports (from 2015 onwards) on the phytochemical composition, beneficial effects and potential applications of citrus fruits and their by-products is systematically summarized. In detail, information regarding the nutraceutical and medicinal value closely linked to the presence of numerous bioactive metabolites and their growing use in the food industry and food packaging, also considering any technological strategies such as encapsulation to guarantee their stability over time, were evaluated. In addition, since citrus fruit, as well as its by-products, are interesting alternatives for the reformulation of natural cosmetic products, the sector of the cosmetic industry is also explored. More in-depth knowledge of the latest information in this field will contribute to future conscious use of citrus fruits.

The use of unmanned marine robotic vehicles in bathymetric surveys is discussed. This paper presents recent results in autonomous bathymetric missions with the ROAZ autonomous surface vehicle. In particular, robotic surface vehicles such as ROAZ provide an efficient tool in risk assessment for shallow water environments and water land interface zones as the near surf zone in marine coast. ROAZ is an ocean capable catamaran for distinct oceanographic missions, and with the goal to fill the gap were other hydrographic surveys vehicles/systems are not compiled to operate, like very shallow water rivers and marine coastline surf zones. Therefore, the use of robotic systems for risk assessment is validated through several missions performed either in river scenario (in a very shallow water conditions) and in marine coastlines.

In this study, the tensile strength of single-lap joints (SLJs) between similar and dissimilar adherends bonded with an acrylic adhesive was evaluated experimentally and numerically. The adherend materials included polyethylene (PE), polypropylene (PP), carbon-epoxy (CFRP), and glass-polyester (GFRP) composites. The following adherend combinations were tested: PE/PE, PE/PP, PE/CFRP, PE/GFRP, PP/PP, CFRP/CFRP, and GFRP/GFRP. One of the objectives of this work was to assess the influence of the adherends stiffness on the strength of the joints since it significantly affects the peel stresses magnitude in the adhesive layer. The experimental results were also used to validate a new mixed-mode cohesive damage model developed to simulate the adhesive layer. Thus, the experimental results were compared with numerical simulations performed in ABAQUS, including a developed mixed-mode (I+II) cohesive damage model, based on the indirect use of fracture mechanics and implemented within interface finite elements. The cohesive laws present a trapezoidal shape with an increasing stress plateau, to reproduce the behaviour of the ductile adhesive used. A good agreement was found between the experimental and numerical results.

Drilling carbon/epoxy laminates is a common operation in manufacturing and assembly. However, it is necessary to adapt the drilling operations to the drilling tools correctly to avoid the high risk of delamination. Delamination can severely affect the mechanical properties of the parts produced. Production of high quality holes with minimal damage is a key challenge. In this article, delamination caused in laminate plates by drilling is evaluated from radiographic images. To accomplish this goal, a novel solution based on an artificial neural network is employed in the analysis of the radiographic images.

This study aims at assessing the influence of the competitive preadsorption of human serum albumin (HSA) and human plasma fibronectin (FN) from binary solutions and 10% plasma on MC3T3-E1 osteoblast adhesion and morphology on two types of TiO2 substrates. One was commercially pure titanium with a titanium oxide layer formed in an H2O2 solution and the other TiO2 sputtered on Si (Sousa et al., Langmuir 2004; 20:9745-9754.). The strategy applied in the present investigation was to compare osteoblast adhesion to surfaces preadsorbed with HSA, FN, HSA/FN = 1, HSA/FN = 200, and 10% plasma. The adsorption of proteins was evaluated measuring the amount and the effectiveness of binding with radiolabeled proteins, 125 I-FN and 125 I-HSA. Our results indicated that MC3T3-E1 osteoblast adhesion correlates well with the amounts of FN and HSA adsorbed on TiO2 surfaces. Also, we found that fewer osteoblasts adhered to both substrates preadsorbed with HSA, HSA/FN = 200, and 10% plasma, after 4 and 24 h, than to the surfaces preadsorbed with FN and HSA/FN = 1. For the latter, FN was able to compensate the inhibitory effect of HSA on osteoblast adhesion. Therefore, the presence of lower amounts of coadsorbed albumin may improve presentation of FN in a more integrin-recognized conformation, suggesting that some degree of molecular packing prevents loss of integrin-binding activity. FN reversibility does not seem to be dependent on the HSA/FN adsorption mass ratio in solution, suggesting that FN competitively adsorbs to TiO2 in a favorable conformation and does not suffers subsequent conformational changes allowing exchange with other FN molecules in solution.

Purpose This paper aims to evaluate the influence of the parameters of the Fused Filament Fabrication (FFF) process on the mechanical properties and on the mass of parts printed in Polylactic Acid (PLA). In addition, the authors developed predictive models for the analysed responses. Design/methodology/approach A full Factorial type of experimental planning method was used to define the conditions for manufacturing parts according to the variation of the construction parameters, extrusion temperature and print speed. Samples were printed for tensile, flexion and compression tests. Their mass was measured. Multiple regression methods, based on power equations, were used to build the forecasting models. Findings It was found that the extrusion temperature was the parameter of greatest influence in the variation of the analysed responses, mainly because it generates behaviour patterns and indirectly demonstrates thermal/rheological characteristics of the material used. Print speed affects responses, however, with variations dependent on part geometry and printer hardware/software. It was possible to establish prediction models with low error rates in relation to the experimental values. Originality/value The study demonstrates a good relation between the use of a structured experimental planning method as the basis for the development of predictive models based on mathematical equations, the same structure of which can be used to describe different responses.

A QuEChERS method has been developed for the determination of 14 organochlorine pesticides in 14 soils from different Portuguese regions with wide range composition. The extracts were analysed by GC-ECD (where GC-ECD is gas chromatography-electron-capture detector) and confirmed by GC-MS/MS (where MS/MS is tandem mass spectrometry). The organic matter content is a key factor in the process efficiency. An optimization was carried out according to soils organic carbon level, divided in two groups: HS (organic carbon >2.3%) and LS (organic carbon <2.3%). The method was validated through linearity, recovery, precision and accuracy studies. The quantification was carried out using a matrix-matched calibration to minimize the existence of the matrix effect. Acceptable recoveries were obtained (70-120%) with a relative standard deviation of ≤16% for the three levels of contamination. The ranges of the limits of detection and of the limits of quantification in soils HS were from 3.42 to 23.77 μg kg(-1) and from 11.41 to 79.23 μg kg(-1), respectively. For LS soils, the limits of detection ranged from 6.11 to 14.78 μg kg(-1) and the limits of quantification from 20.37 to 49.27 μg kg(-1) . In the 14 collected soil samples only one showed a residue of dieldrin (45.36 μg kg(-1) ) above the limit of quantification. This methodology combines the advantages of QuEChERS, GC-ECD detection and GC-MS/MS confirmation producing a very rapid, sensitive and reliable procedure which can be applied in routine analytical laboratories.