Laboratoire de Mécanique des Fluides de Lille - Kampé de Fériet

The objectives of this study were to evaluate the capacity of 2 dietary feed additives, sodium bicarbonate and live yeast Saccharomyces cerevisiae (strain Sc 47), in optimizing ruminal pH in dairy cows and to determine their modes of action. Three early lactating Holstein cows, fitted with ruminal cannulas, were allocated in a 3 x 3 Latin square design. They were given a total mixed ration as control diet (CD) at a daily feeding rate of 28.0 kg of dry matter (DM)/cow supplemented with 150 g/d of sodium bicarbonate (SBD) or 5 g/d of live yeast (YD) during a 21-d experimental period (14 d of diet adaptation, 4 consecutive days of measurement and sampling and 3 d of transition). The pH and redox potential (E(h)) were measured from 1 h before feeding to 8 h after feeding at 1-h intervals, and samples of ruminal fluid were taken at 0, 2, 4, 6, and 8 h after feeding for the determination of volatile fatty acids and lactate concentrations. Total tract apparent digestibility of the diet was also determined. Ruminal pH fluctuated between 6.53 at feeding and 5.57 at 5 h postfeeding. Mean pH was greater with SBD (6.21) and YD (6.14) compared with CD (5.94), showing that both additives had a pH stabilization effect. The E(h) varied from -88 mV at 1 h before feeding to -165 mV at 1 h after feeding. Mean E(h) and Clark's Exponent (rH) were lower with YD (-149 mV and 7.31, respectively) than with SBD (-137 mV and 7.85, respectively) and CD (-115 mV and 8.05, respectively), indicating that the yeast strengthened the reducing power of the milieu. Total volatile fatty acids were greater in SBD (95.3 mM) and YD (99.4 mM) compared with CD (85.3 mM). Acetate concentration was greater in SBD (60.8 mM) and YD (59.1 mM) compared with CD (53.2 mM). Propionate concentration was greater in YD (25.8 mM) than in SBD (20.0 mM) and CD (18.0 mM). Butyrate remained constant between diets. Mean total lactate concentrations were 16.5, 12.2, and 5.4 mM for CD, SBD, and YD, respectively, with a 67% decrease with YD. Total tract organic matter digestibility was greater for YD (66.6%) compared with SBD (61.7%) and CD (62.2%). The neutral detergent fiber digestibility was greater with YD (41.6%) compared with SBD (34.3%) and CD (29.6%), whereas acid detergent fiber digestibility was greatest in YD (32.3%), intermediate in SBD (24.4%), and lowest in CD (18.1%). By inducing a lower ruminal E(h) and rH, live yeast prevented accumulation of lactate and allowed better fiber digestion, whereas sodium bicarbonate seemed to act only as an exogenous buffer.

This paper concerns the computation of derivatives from particle image velocimetry (PIV) velocity fields with the goal of obtaining the vorticity component normal to the plane. A variety of derivative schemes are characterized by their transfer function, taking into account the truncation and noise amplification. The PIV measurement noise is supposed to be a white one in the Fourier space. A spectral approach is used in order to choose the best filter for turbulent flows. The derivative spectra are discussed. An application is presented on a real turbulent flow with two interrogation window sizes and different derivative schemes. The most significant schemes are also applied to a velocity field containing a single vortex. A comparison of the maximum of vorticity obtained with each scheme and through a least-square fit with an Oseen vortex, allows us to quantify the effect of the band pass filter and to select the best scheme.

Abstract The linear global instability and resulting transition to turbulence induced by an isolated cylindrical roughness element of height $h$ and diameter $d$ immersed within an incompressible boundary layer flow along a flat plate is investigated using the joint application of direct numerical simulations and fully three-dimensional global stability analyses. For the range of parameters investigated, base flow computations show that the roughness element induces a wake composed of a central low-speed region surrounded by a three-dimensional shear layer and a pair of low- and high-speed streaks on each of its sides. Results from the global stability analyses highlight the unstable nature of the central low-speed region and its crucial importance in the laminar–turbulent transition process. It is able to sustain two different global instabilities: a sinuous and a varicose one. Each of these globally unstable modes is related to a different physical mechanism. While the varicose mode has its root in the instability of the whole three-dimensional shear layer surrounding the central low-speed region, the sinuous instability turns out to be similar to the von Kármán instability in the two-dimensional cylinder wake and has its root in the lateral shear layers of the separated zone. The aspect ratio of the roughness element plays a key role on the selection of the dominant instability: whereas the flow over thin cylindrical roughness elements transitions due to a sinuous instability of the near-wake region, for larger roughness elements the varicose instability of the central low-speed region turns out to be the dominant one. Direct numerical simulations of the flow past an aspect ratio ${\it\eta}=1$ (with ${\it\eta}=d/h$ ) roughness element sustaining only the sinuous instability have revealed that the bifurcation occurring in this particular case is supercritical. Finally, comparison of the transition thresholds predicted by global linear stability analyses with the von Doenhoff–Braslow transition diagram provides qualitatively good agreement.

The goal of the work is to investigate the possibility of cavitation erosion prediction using computational fluid dynamics (CFD) tools only. For that purpose, a numerical process based on a coupling between CFD and an erosion model is presented and tested in several configurations of cavitating flow on a two-dimensional hydrofoil. The CFD code, which is based on the homogeneous approach, was previously validated on numerous experiments. In the present work, the predictions of velocity and pressure evolutions in the vicinity of the hydrofoil are compared with experimentally measured data. A close agreement is systematically obtained. The erosion model is based on the physical description of phenomena from cavitation cloud implosion, pressure wave emission and its attenuation, micro-jet formation and finally to the pit formation. The coupling between CFD and the erosion model is based on the use of local pressure, void fraction and velocity values to determine the magnitude of damage at a certain point. The results are compared with the experimentally measured damage on the hydrofoil. In the experiments a thin copper foil applied to the surface of the hydrofoil was used as an erosion sensor. A pit-count method was applied to evaluate the damage. The comparison shows that it is possible to use solely CFD tools to predict time evolution of cavitation erosion, including final extent and magnitude, with a very good accuracy. Copyright © 2009 John Wiley & Sons, Ltd.

Exploiting pattern formation - such as that observed in nature - in the context of micro/nanotechnology could have great benefits if coupled with the traditional top-down lithographic approach. Here, we demonstrate an original and simple method to produce unique, localized and controllable self-organised patterns on elastomeric films. A thin, brittle silica-like crust is formed on the surface of polydimethylsiloxane (PDMS) using oxygen plasma. This crust is subsequently cracked via the deposition of a thin metal film - having residual tensile stress. The density of the mud-crack patterns depends on the plasma dose and on the metal thickness. The mud-crack patterning can be controlled depending on the thickness and shape of the metallization - ultimately leading to regularly spaced cracks and/or metal mesa structures. Such patterning of the cracks indicates a level of self-organization in the structuring and layout of the features - arrived at simply by imposing metallization boundaries in proximity to each other, separated by a distance of the order of the critical dimension of the pattern size apparent in the large surface mud-crack patterns.

International audience

This paper provides new results describing compressible fluid flow around a cylinder. The investigation was restricted to subsonic and transonic flow at Reynolds numbers of about 10s. The experiments showed that a strong coupling exists between the flow over a cylinder and the vortex street formed in the near wake. The phenomenon was investigated using high-speed visualization synchronized with unsteady pressure measurements. Various coupling regimes were classified and instantaneous pressure distributions were obtained at different times during the vortex street period. From these elements it was possible to deduce the unsteady force.

Abstract Compaction of concrete is physically a collapse of the material porous microstructure. It produces plastic strains in the material and, at the same time, an increase of its bulk modulus. This paper presents two experimental techniques aimed at obtaining the hydrostatic response of concrete and mortar. The first one is a uniaxial confined compression test which is quite simple to implement and allows to reach hydrostatic pressures of about 600 MPa. The specimen size is large enough so that concrete with aggregate sizes up to 16 mm can be tested. The second one is a true hydrostatic test performed on smaller (mortar) specimens. Test results show that the hydrostatic response of the material is elasto‐plastic with a stiffening effect on both the tangent and unloading bulk moduli. The magnitude of the irreversible volumetric strains depends on the initial porosity of the material. This porosity can be related in a first approximation to the water/cement ratio. A comparison of the hydrostatic responses obtained from the two testing techniques on the same material show that the hydrostatic response of cementitious materials cannot be uncoupled from the deviatoric response, as opposed to the standard assumption in constitutive relations for metal alloys. This feature should be taken into account in the development of constitutive relations for concrete subjected to high confinement pressures which are needed in the modelling of impact problems. Copyright © 2001 John Wiley & Sons, Ltd.

A direct numerical simulation of the incompressible Navier–Stokes equations of the flow over a bump shows a stationary longitudinal instability at a Reynolds number of Re = 400. A three-dimensional global mode linear analysis is used to interpret these results and shows that the most unstable eigenmode is steady and localized in the recirculation bubble, with spanwise wavelength of approximately ten bump heights. An inviscid geometrical optics analysis along closed streamlines is then proposed and modified to account for viscous effects. This motivates a final discussion regarding the physical origin of the observed instability.

We study the dynamics of neutrally buoyant particles with diameters varying in the range [1, 45] in Kolmogorov scale units (η) and Reynolds numbers based on Taylor scale ( Re λ ) between 590 and 1050. One component of the particle velocity is measured using an extended laser Doppler velocimetry at the centre of a von Kármán flow, and acceleration is derived by differentiation. We find that the particle acceleration variance decreases with increasing diameter with scaling close to ( D /η) −2/3 , in agreement with previous observations, and with a hint for an intermittent correction as suggested by arguments based on scaling of pressure spatial increments. The characteristic time of acceleration autocorrelation increases more strongly than previously reported in other experiments, and possibly varying linearly with D /η. Further analysis shows that the probability density functions of the acceleration have smaller wings for larger particles; their flatness decreases as well, as expected from the behaviour of pressure increments in turbulence when intermittency corrections are taken into account. We contrast our measurements with previous observations in wind-tunnel turbulent flows and numerical simulations.

Residual stresses generated in coatings during thermal spraying could have very different inside intensity and distribution, depending on the materials and processing conditions. As it is recognised that residual stresses play a major role in the adhesion of coatings, it is necessaryto evaluate precisely their influence. It is not possible to conduct these measurements directly, and no indication on how the stresses should be taken into account has been reported in the literature. Moreover, depending on the test used to evaluate adhesion, different volumes of the coatingcan participate in the delamination process. In order to take into account these observations, it is proposed to define two stress parameters related either to the stresses in the coating or to the stresses at the interfacial zone. In these conditions, it is possible to explain the variationin adhesion as a function of the coating thickness, i.e. to explain the maximum value obtained for the bonding strength deduced from tensile tests and the monotonic increase in adhesion toughness deduced from interfacial indentation tests.

Lipid accumulation alters macrophage biology and contributes to lipid retention within the vessel wall. In this study, we investigated the role of adipophilin on triglyceride accumulation and lipid-droplet formation in THP-1-derived macrophages (THP-1 macrophages). In the presence of acetylated low-density lipoprotein, macrophages infected with an adenovirus expressing human adipophilin showed a 31% increase in triglyceride content and a greater number of lipid droplets compared with control cells. Incubation of macrophages with very low-density lipoprotein (VLDL) dramatically increased cellular triglyceride content similarly in control and adipophilin-overexpressing cells. By itself, VLDL increased adipophilin expression, which explains the lack of effect of adipophilin overexpression on cellular triglyceride content in macrophages loaded with VLDL. The lipid-droplet content of macrophages was increased by overexpression of adipophilin and/or loading with VLDL. In contrast, inhibition of adipophilin expression using siRNA prevented lipid-droplet formation and significantly reduced intracellular triglyceride content. Using inhibitors of beta-oxidation and acyl-coenzyme A synthetase, results were obtained which suggest that adipophilin elevates cellular lipids by inhibition of beta-oxidation and stimulation of long-chain fatty acid incorporation into triglycerides. Adipophilin expression in THP-1 macrophages altered the cellular content of different lipids and enhanced the size of lipid droplets, consistent with a role for adipophilin in human foam cell formation.

OBJECTIVES: To study if genes with common single nucleotide polymorphisms (SNPs) associated with obesity-related phenotypes influence weight loss (WL) in obese individuals treated by a hypo-energetic low-fat or high-fat diet. DESIGN: Randomised, parallel, two-arm, open-label multi-centre trial. SETTING: Eight clinical centres in seven European countries. PARTICIPANTS: 771 obese adult individuals. INTERVENTIONS: 10-wk dietary intervention to hypo-energetic (-600 kcal/d) diets with a targeted fat energy of 20%-25% or 40%-45%, completed in 648 participants. OUTCOME MEASURES: WL during the 10 wk in relation to genotypes of 42 SNPs in 26 candidate genes, probably associated with hypothalamic regulation of appetite, efficiency of energy expenditure, regulation of adipocyte differentiation and function, lipid and glucose metabolism, or production of adipocytokines, determined in 642 participants. RESULTS: Compared with the noncarriers of each of the SNPs, and after adjusting for gender, age, baseline weight and centre, heterozygotes showed WL differences that ranged from -0.6 to 0.8 kg, and homozygotes, from -0.7 to 3.1 kg. Genotype-dependent additional WL on low-fat diet ranged from 1.9 to -1.6 kg in heterozygotes, and from 3.8 kg to -2.1 kg in homozygotes relative to the noncarriers. Considering the multiple testing conducted, none of the associations was statistically significant. CONCLUSIONS: Polymorphisms in a panel of obesity-related candidate genes play a minor role, if any, in modulating weight changes induced by a moderate hypo-energetic low-fat or high-fat diet.

We report on the Lagrangian statistics of acceleration of small (sub-Kolmogorov) bubbles and tracer particles with Stokes number St≪1 in turbulent flow. At a decreasing Reynolds number, the bubble accelerations show deviations from that of tracer particles; i.e., they deviate from the Heisenberg-Yaglom prediction and show a quicker decorrelation despite their small size and minute St. Using direct numerical simulations, we show that these effects arise due the drift of these particles through the turbulent flow. We theoretically predict this gravity-driven effect for developed isotropic turbulence, with the ratio of Stokes to Froude number or equivalently the particle drift velocity governing the enhancement of acceleration variance and the reductions in correlation time and intermittency. Our predictions are in good agreement with experimental and numerical results. The present findings are relevant to a range of scenarios encompassing tiny bubbles and droplets that drift through the turbulent oceans and the atmosphere. They also question the common usage of microbubbles and microdroplets as tracers in turbulence research.

A theoretical analysis of the fast transients of turbomachineries, based on the study of unsteady and incompressible fluids mechanics equations applied to an impeller, is proposed. It leads to internal torque, internal power, and impeller head of an impeller during transient periods. The equations show that the behavior of a pump impeller is not only depending on the acceleration rate and flow rate, as it is usually admitted, but also on velocity profiles and their evolution during the transient. Some hypotheses on the flow in a radial flow pump are proposed. They are validated by comparison with the experimental results of a single stage, single volute radial flow pump during some fast acceleration periods. The model is also used to analyze the behavior of the pump during a fast startup.

ABSTRACT Strain field measurements by digital image correlation today offer new possibilities for analysing the mechanical behaviour of materials in situ during mechanical tests. The originality of the present study is to use this technique on the micro‐structural scale, in order to understand and to obtain quantitative values of the fatigue surface damage in a two‐phased alloy. In this paper, low‐cycle fatigue damage micromechanisms in an austenitic‐ferritic stainless steel are studied. Surface damage is observed in real time, with an in situ microscopic device, during a low‐cycle fatigue test performed at room temperature. Surface displacement and strain fields are calculated using digital image correlation from images taken during cycling. A detailed analysis of optical images and strain fields measured enables us to follow precisely the evolution of surface strain fields and the damage micromechanisms. Firstly, strain heterogeneities are observed in austenitic grains. Initially, the austenitic phase accommodates the cyclic plastic strain and is then followed by the ferritic phase. Microcrack initiation takes place at the ferrite/ferrite grain boundaries. Microcracks propagate to the neighbouring austenitic grains following the slip markings. Displacement and strain gradients indicate probable microcrack initiation sites.

In rotating machinery, notably in modern high efficiency compressors, a critical requirement for optimal performance consists in minimizing radial clearances between the rotating bladed disk and the casing. This solution significantly increases the risks of contact between rotating bladed disk and casing and may lead in specific conditions to catastrophic behavior (component failure, etc.). The physical phenomena and mechanisms involved in blade-casing contact interaction situations are still misunderstood. In order to highlight these mechanisms, specific experiments have been performed on an experimental multi-stage compressor of a turbojet with dedicated dynamic and thermal instrumentations. For all configurations tested, major damages are noticed: blades had cracks and the abradable coating of the casing was heavily machined. Results show that the blade failure refers to fatigue limit with first natural mode excitation of the blade. The paper is focused on the analysis of the successive stages of blade dynamic response before the failure. It is shown that this response is influenced by the variations of the blade-casing contact conditions. These conditions are linked to the thermomechanical behavior and wear of coating, illustrated by high thermal levels and non uniform wear profile. Coupling between thermomechanics, wear and dynamic has to be considered to highlight the transient mechanisms leading to the cases of blade failure.

For numerical simulations of cavitating flows, many physical models are currently used. One approach is the void fraction transport equation‐based model including source terms for vaporization and condensation processes. Various source terms have been proposed by different researchers. However, they have been tested only in different flow configurations, which make direct comparisons between the results difficult. A comparative study, based on the expression of the source terms as a function of the pressure, is presented in the present paper. This analytical approach demonstrates a large resemblance between the models, and it also clarifies the influence of the model parameters on the vaporization and condensation terms and, therefore, on the cavity shape and behavior. Some of the models were also tested using a 2D CFD code in configurations of cavitation on two‐dimensional foil sections. Void fraction distributions and frequency of the cavity oscillations were compared to existing experimental measurements. These numerical results confirm the analytical study.

The paper presents an experimental investigationof the effect of the trailing edge vortex shedding on the steady and unsteady trailing blade pressure distribution of a turbine blade at high subsonic Mach number M2,is=0.79 and high Reynolds number RE=2.8×106. The vortex formation and shedding process is visualized using a high-speed schlieren camera and a holographic interferometric density measuring technique. The blade is equipped with a rotatable trailing edge cylinder instrumented side-by-side with a pneumatic pressure tap and a fast response pressure sensor for detailed measurements of the trailing edge pressure distribution. The experiments demonstrate that contrary to the isobaric dead air region demonstrated at low subsonic Mach numbers the data reveal the existence of a highly nonuniform trailing edge pressure distribution with a strong pressure minimum at the center of the trailing edge. This finding is significant for the determination of the base pressure coefficient that is in general measured with a single pressure-sensing hole at the trailing edge center. The paper investigates further the effect of the vortex shedding on the blade rear suction side and discusses the superposition of unsteady effects emanating from the trailing edge and from the neighboring blade. The experimental data are a unique source for the validation of unsteady Navier-Stokes codes.

New expressions of the macroscopic criteria of perfectly plastic rigid matrix containing prolate and oblate cavities are presented. The proposed approach, derived in the framework of limit analysis, consists in the consideration of Eshelby-like trial velocity fields for the determination of the macroscopic dissipation. It is shown that the obtained results significantly improve existing criteria for ductile porous media. Moreover, for low stress triaxialities, these new results also agree perfectly with the (nonlinear) Hashin–Shtrikhman bound established by Ponte-Castañeda and Suquet.