Ministère des Ressources naturelles et des Forêts (Québec)

BACKGROUND: DNA barcoding aims to provide an efficient method for species-level identifications using an array of species specific molecular tags derived from the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene. The efficiency of the method hinges on the degree of sequence divergence among species and species-level identifications are relatively straightforward when the average genetic distance among individuals within a species does not exceed the average genetic distance between sister species. Fishes constitute a highly diverse group of vertebrates that exhibit deep phenotypic changes during development. In this context, the identification of fish species is challenging and DNA barcoding provide new perspectives in ecology and systematics of fishes. Here we examined the degree to which DNA barcoding discriminate freshwater fish species from the well-known Canadian fauna, which currently encompasses nearly 200 species, some which are of high economic value like salmons and sturgeons. METHODOLOGY/PRINCIPAL FINDINGS: We bi-directionally sequenced the standard 652 bp "barcode" region of COI for 1360 individuals belonging to 190 of the 203 Canadian freshwater fish species (95%). Most species were represented by multiple individuals (7.6 on average), the majority of which were retained as voucher specimens. The average genetic distance was 27 fold higher between species than within species, as K2P distance estimates averaged 8.3% among congeners and only 0.3% among concpecifics. However, shared polymorphism between sister-species was detected in 15 species (8% of the cases). The distribution of K2P distance between individuals and species overlapped and identifications were only possible to species group using DNA barcodes in these cases. Conversely, deep hidden genetic divergence was revealed within two species, suggesting the presence of cryptic species. CONCLUSIONS/SIGNIFICANCE: The present study evidenced that freshwater fish species can be efficiently identified through the use of DNA barcoding, especially the species complex of small-sized species, and that the present COI library can be used for subsequent applications in ecology and systematics.

Although understood in many vertebrate systems, the natural diversity of host-associated microbiota has been little studied in teleosts. For migratory fishes, successful exploitation of multiple habitats may affect and be affected by the composition of the intestinal microbiome. We collected 96 Salmo salar from across the Atlantic encompassing both freshwater and marine phases. Dramatic differences between environmental and gut bacterial communities were observed. Furthermore, community composition was not significantly impacted by geography. Instead life-cycle stage strongly defined both the diversity and identity of microbial assemblages in the gut, with evidence for community destabilisation in migratory phases. Mycoplasmataceae phylotypes were abundantly recovered in all life-cycle stages. Patterns of Mycoplasmataceae phylotype recruitment to the intestinal microbial community among sites and life-cycle stages support a dual role for deterministic and stochastic processes in defining the composition of the S. salar gut microbiome.

This paper reviews aspects of the functional ecology of naturally established tree seedlings in the boreal forests of North America with an emphasis on the relationship between light availability and the growth and survival of shade tolerant conifers up to pole size. Shade tolerant conifer species such as firs and spruces tend to have a lower specific leaf mass, photosynthetic rate at saturation, live crown ratio, STAR (shoot silhouette area to total needle surface area ratio), and root to shoot ratio than the shade intolerant pines. The inability of intolerant species such as the pines and aspen to survive in shade appears to be mainly the result of characteristics at the shoot, crown, and whole-tree levels and not at the leaf level. Although firs and spruces frequently coexist in shaded understories, they do not have identical growth patterns and crown architectures. We propose a simple framework based on the maximum height that different tree species can sustain in shade, which may help managers determine the timing of partial or complete harvests. Consideration of these functional aspects of regeneration is important to the understanding of boreal forest dynamics and can be useful to forest managers seeking to develop or assess novel silvicultural systems.

Relationships between soil organic carbon (SOC), organic matter (SOM), and bulk density (BD) were established in acidic loamy to sandy loam fine fractions of forest soils in Quebec (Canada). The interest of such relationships rests with the possibility of using simple and rapid techniques to estimate SOC and BD. It is also a crucial step in establishing the correspondence among several databases when SOC data are obtained using different measurement techniques. In this study, SOC was measuredby dry combustion (SOCN DC ) and wet digestion (SOC WD ) methods, and organic matter by loss-on-ignition (LOI). Our results suggest that, in these soils: (1) LOI can be used for estimating SOC (r 2 = 0.95, RMSEP = 16%) and SOC DC /SOM significantly decreased with increasing depth from 0.49 to 0.27; (2) SOC DC and SOC WD were highly correlated. Even if SOC WD provided near complete recovery of SOC DC , dry combustion remains the preferred method for SOC analysis since recovery decreased with increasing depth from 100 to 83%. (3) BD was also strongly related to SOM(r 2 = 0.81). We recommend using the organic density approach to estimate BD from SOM because it allows BD to be predicted without significant bias and with a degree of accuracy of 14%. Key words: Forest soils, soil organic carbon, soil organic matter, soil bulk density

Habitat use patterns of 3 species of temperate eels, Anguilla anguilla, A. japonica and A. rostrata, were investigated using otolith strontium:calcium ratio life history transects. Published and unpublished data from 6 sites (Canada, United States, Sweden, France, Taiwan and Japan) sampled across the geographical range of each eel species were compiled. Sr:Ca patterns indicated that the 3 species displayed similar patterns of habitat use. In all sites, patterns of habitat use consisted of either residency in one habitat (fresh, brackish, or marine) or movements between habitats. One movement pattern consisted of either a single change or 2 changes of habitat from fresh to brackish waters, or from brackish water to freshwater. Seasonal movements between fresh and brackish waters were observed for all 3 species. When only a single habitat switch event was detected, it occurred between 3 and 5 yr of age. Occurrence of eels with no freshwater experience was demonstrated, but such eels accounted for a smaller proportion of the overall sample than eels with some (even brief) freshwater experience. Contrary to the common convention that these are obligate catadromous species, we must now consider them as facultative catadromous, with far more flexibility in habitat use. The most variable parameter among study sites was the relative proportion, rather than the diversity, of lifetime spent in the various habitat use patterns. Eels found at higher latitudes exhibited a greater probability of remaining in the lower reaches of watersheds in brackish water. Diversity of habitat use appears to be a common strategy of temperate eel species, and, as a life history tactic, is under environmental control.

Increasing accumulation of CO 2 in the atmosphere has led to calls for terrestrial mechanisms for CO 2 abatement and given that soils represent the largest terrestrial body of C on Earth, there is a great deal of interest in soils as a sink for atmospheric C. This emphasis on sequestration in boreal forest soils is understandable given the sheer mass of this C reservoir (~1700 Pg of C) but diverts our attention from the importance of soil C in soil physical, chemical and biotic functions, and importantly, it ignores the possibility that soils may also represent a source of C. In this review, we address these issues through a discussion of the size and character of boreal forest soil C pool, its role in ecosystem function, the potential impacts of climate change on soil C, efforts to model these processes and the role of soil C in boreal resilience to the impacts of climate change. Soil C is fundamental to ecosystem function in terms of improving soil physical properties, increasing soil biotic activity and enhancing insulation all of which improve site productivity. Managing upland soils for C sequesteration will achieve little in terms of offsetting fossil fuel emissions but would likely improve soil quality. Most of the C stored in the boreal biome is found in permafrost and wetland soils and events related to climatic change could shift these soils from C sink to C source. Melting of permafrost soils with predicted warming trends within the circumpolar region could result in the release of 30-60 Pg C by the year 2040. Such predictions, however, are limited by uncertainty in both climatic changes and soil response to these changes. Prediction of shifts in soil C dynamics with climate change relies on our ability to link C transformations to N dynamics and climatic variables. Improvement in ecosystem models will advance our ability to assess the resilience of the boreal biome under future climatic conditions.

Models of greenstone belt development are crucial for exploration. Allochthonous models predict belts to be a collage of unrelated fragments, whereas autochthonous models allow for prediction of syngenetic mineral deposits within specific stratigraphic intervals. Superior province greenstone belts consist of mainly volcanic units unconformably overlain by largely sedimentary “Timiskaming-style” assemblages, and field and geochronological data indicate that the Abitibi greenstone belt developed autochthonously. We describe major revisions to stratigraphy of the Abitibi greenstone belt and the implications of an autochthonous development of the volcanic stratigraphy for exploration for syngenetic mineralization. The Abitibi greenstone belt is subdivided into seven discrete volcanic stratigraphic episodes on the basis of groupings of numerous U-Pb zircon ages of pre-2750, 2750 to 2735, 2734 to 2724, 2723 to 2720, 2719 to 2711, 2710 to 2704, and 2704 to 2695 Ma. We present revised lithotectonic and/or stratigraphic nomenclature using these time intervals, including (1) isotopic inheritance in younger episodes which indicates that the older episodes (2750–2735 and 2734–2724 Ma) had greater extent than is presently seen, (2) dikes feeding younger volcanic episodes (2706 Ma) cutting older volcanic units (2734–2724 Ma), and (3) 2710 to 2704 Ma mafic to ultramafic sills intruding the 2719 to 2711 Ma episode. Changes to the nomenclature include the identification of pre-2750 Ma volcanic episode (supracrustal fragments) in the northern and southern Abitibi greenstone belt and subdivision of the 2719 to 2711 Ma, 2710 to 2704 Ma, and 2704 to 2695 Ma episodes into lower and upper parts. We present the results of this lithostratigraphic subdivision as the first geochronologically constrained stratigraphic and/or lithotectonic map of the Abitibi greenstone belt. Many of the volcanic episodes are intercalated with and capped by a relatively thin “sedimentary interface zone” dominated by chemical sedimentary rocks. Stratigraphic and geochronological analysis of these zones indicates discontinuous deposition with localized gaps of 2 to 27 m.y. between volcanic episodes. The zones consist of up to 200 m of iron formation, chert breccia, heterolithic debris flows of volcanic provenance, sandstone and/or argillite and conglomerate. Modeling of the time required for deposition of the volcanic units based on rates of magma production in modern arc and plume environments is on the order of 103 to 104 years, whereas the time interval between basalt-rhyolite cycles is 106 years. The sedimentary interface zones are therefore interpreted as condensed sections, zones with very low rates of sedimentation in a basinal setting, or zones with negligible rates of sedimentation marked by silicification of existing rock types. The sedimentary interface zones are therefore considered submarine correlative conformities, disconformities, or unconformities separating the equivalent of group level stratigraphic and lithotectonic units. The unconformity-bounded stratigraphic model provides a new regional to deposit-scale interpretive model for use in exploration for syngenetic mineralization.

Disentangling evolutionary forces that may interact to determine the patterns of genetic differentiation within and among wild populations is a major challenge in evolutionary biology. The objective of this study was to assess the genetic structure and the potential influence of several ecological variables on the extent of genetic differentiation at multiple spatial scales in a widely distributed species, the Atlantic salmon, Salmo salar. A total of 2775 anadromous fish were sampled from 51 rivers along the North American Atlantic coast and were genotyped using 13 microsatellites. A Bayesian analysis clustered these populations into seven genetically and geographically distinct groups, characterized by different environmental and ecological factors, mainly temperature. These groups were also characterized by different extent of genetic differentiation among populations. Dispersal was relatively high and of the same magnitude within compared to among regional groups, which contrasted with the maintenance of a regional genetic structure. However, genetic differentiation was lower among populations exchanging similar rates of local as opposed to inter-regional migrants, over the same geographical scale. This raised the hypothesis that gene flow could be constrained by local adaptation at the regional scale. Both coastal distance and temperature regime were found to influence the observed genetic structure according to landscape genetic analyses. The influence of other factors such as latitude, river length and altitude, migration tactic, and stocking was not significant at any spatial scale. Overall, these results suggested that the interaction between gene flow and thermal regime adaptation mainly explained the hierarchical genetic structure observed among Atlantic salmon populations.

Soaring birds migrate in massive numbers worldwide. These migrations are complex and dynamic phenomena, strongly influenced by meteorological conditions that produce thermal and orographic uplift as the birds traverse the landscape. Herein we report on how methods were developed to estimate the strength of thermal and orographic uplift using publicly available digital weather and topography datasets at continental scale. We apply these methods to contrast flight strategies of two morphologically similar but behaviourally different species: golden eagle, Aquila chrysaetos, and turkey vulture, Cathartes aura, during autumn migration across eastern North America tracked using GPS tags. We show that turkey vultures nearly exclusively used thermal lift, whereas golden eagles primarily use orographic lift during migration. It has not been shown previously that migration tracks are affected by species-specific specialisation to a particular uplift mode. The methods introduced herein to estimate uplift components and test for differences in weather use can be applied to study movement of any soaring species.

In vertebrates, variability at genes of the Major Histocompatibility Complex (MHC) represents an important adaptation for pathogen resistance, whereby high allelic diversity confers resistance to a greater number of pathogens. Pathogens can maintain diversifying selection pressure on their host's immune system that can vary in intensity based on pathogen richness, pathogen virulence, and length of the cohabitation period, which tend to increase with temperature. In this study, we tested the hypothesis that genetic diversity of MHC increases with temperature along a latitudinal gradient in response to pathogen selective pressure in the wild. A total of 1549 Atlantic salmon from 34 rivers were sampled between 46 degrees N and 58 degrees N in Eastern Canada. The results supported our working hypothesis. In contrast to the overall pattern observed at microsatellites, MHC class II allelic diversity increased with temperature, thus creating a latitudinal gradient. The observed temperature gradient was more pronounced for MHC amino acids of the peptide-binding region (PBR), a region that specifically binds to pathogens, than for the non-PBR. For the subset of rivers analyzed for bacterial diversity, MHC amino acid diversity of the PBR also increased significantly with bacterial diversity in each river. A comparison of the relative influence of temperature and bacterial diversity revealed that the latter could have a predominant role on MHC PBR variability. However, temperature was also identified as an important selective agent maintaining MHC diversity in the wild. Based on the bacteria results and given the putative role of temperature in shaping large-scale patterns of pathogen diversity and virulence, bacterial diversity is a plausible selection mechanism explaining the observed association between temperature and MHC variability. Therefore, we propose that genetic diversity at MHC class II represents local adaptation to cope with pathogen diversity in rivers associated with different thermal regimes. This study illuminates the link between selection pressure from the environment, host immune adaptation, and the large-scale genetic population structure for a nonmodel vertebrate in the wild.

Climate change is altering insect disturbance regimes via temperature‐mediated phenological changes and trophic interactions among host trees, herbivorous insects, and their natural enemies in boreal forests. Range expansion and increase in outbreak severity of forest insects are occurring in Europe and North America. The degree to which northern forest ecosystems are resilient to novel disturbance regimes will have direct consequences for the provisioning of goods and services from these forests and for long‐term forest management planning. Among major ecological disturbance agents in the boreal forests of North America is a tortricid moth, the eastern spruce budworm, which defoliates fir ( Abies spp.) and spruce ( Picea spp.). Northern expansion of this defoliator in eastern North America and climate‐induced narrowing of the phenological mismatch between the insect and its secondary host, black spruce ( Picea mariana ), may permit greater defoliation and mortality in extensive northern black spruce forests. Although spruce budworm outbreak centers have appeared in the boreal black spruce zone historically, defoliation and mortality were minor. Potential increases in outbreak severity and tree mortality raise concerns about the future state of this northern ecosystem. Severe spruce budworm outbreaks could decrease stand productivity compared with their occurrence in more diverse, southern balsam fir forest landscapes that have coevolved with outbreaks. Furthermore, depending on the proportion of balsam fir and deciduous species present and fire recurrence, changes in regeneration patterns and in nutrient cycling could alter ecosystem dynamics and replace black spruce by more productive mixed‐wood forest, or by less productive ericaceous shrublands. Long‐term monitoring, manipulative experiments, and process modeling of climate‐induced phenological changes on herbivorous insect pests, their host tree species, and natural enemies in northern forests are therefore crucial to predicting species range shifts and assessing ecological and economic impacts.

Summary The density of large herbivores is a major driver of forest ecosystem structure and function in conjunction with episodic disturbances, especially in forests with a regeneration strategy based on shade‐tolerant seedlings capable of re‐establishing canopy dominance (advance regeneration). Yet, uncertainty about the relationships between forest regeneration, herbivore density and other disturbances makes it difficult to set population goals. Using an innovative controlled browsing experiment, we investigated the relationships between the regeneration dynamics of balsam fir Abies balsamea , the density of white‐tailed deer Odocoileus virginianus and timber harvesting. We hypothesize that advance tree regeneration either: (i) recovers approximately linearly as deer density is reduced; (ii) recovers exponentially; or (iii) does not recover because factors other than browsing control advance regeneration. We tested these alternatives through manipulation of deer densities (0, 7·5, 15 deer km −2 and in situ local densities) and forest cover (clearcut and uncut forest). Balsam fir seedling mortality decreased exponentially with decreasing deer density in clearcut and approximately linearly in uncut forest. Independently of deer density, the recruitment of seedlings in clearcut dropped from 56 ± 5% to 7 ± 1% within 3 years. Seedling growth increased exponentially with decreasing deer density in clearcut whereas no height growth was observed in uncut forest. Overall, the abundance of fir saplings recovered exponentially in clearcut but remained low and independent of deer density in uncut forest. The abundance of spruce Picea spp. saplings was unrelated to deer density and increased with time. Synthesis and applications . Forest disturbance from selective browsing at high deer densities over an extended period of time leads to recruitment failure following a canopy disturbance such as a clearcut. Indirect competitive advantage given to species resistant to browsing can shift forest composition. Nonlinear relationships between fir regeneration and deer densities imply that the level of culling required to reach herbivore densities compatible with natural regeneration of native forest is larger than expected if tree regeneration was proportional to deer density. In the boreal forest of Anticosti Island, local densities < 15 deer km −2 achieved within 3 years following clearcut are compatible with the maintenance of native forest.

The involvement of two R2R3-MYB genes from Pinus taeda L., PtMYB1 and PtMYB8, in phenylpropanoid metabolism and secondary cell wall biogenesis was investigated in planta. These pine MYBs were constitutively overexpressed (OE) in Picea glauca (Moench) Voss, used as a heterologous conifer expression system. Morphological, histological, chemical (lignin and soluble phenols), and transcriptional analyses, i.e. microarray and reverse transcription quantitative PCR (RT-qPCR) were used for extensive phenotyping of MYB-overexpressing spruce plantlets. Upon germination of somatic embryos, root growth was reduced in both transgenics. Enhanced lignin deposition was also a common feature but ectopic secondary cell wall deposition was more strongly associated with PtMYB8-OE. Microarray and RT-qPCR data showed that overexpression of each MYB led to an overlapping up-regulation of many genes encoding phenylpropanoid enzymes involved in lignin monomer synthesis, while misregulation of several cell wall-related genes and other MYB transcription factors was specifically associated with PtMYB8-OE. Together, the results suggest that MYB1 and MYB8 may be part of a conserved transcriptional network involved in secondary cell wall deposition in conifers.

The species of the genus Populus, collectively known as poplars, are widely distributed over the northern hemisphere and well known for their ecological, economical, and evolutionary importance. The extensive interspecific hybridization and high morphological diversity in this group pose difficulties in identifying taxonomic units for comparative evolutionary studies and systematics. To understand the evolutionary relationships among poplars and to provide a framework for biosystematic classification, we reconstructed a phylogeny of the genus Populus based on nucleotide sequences of three noncoding regions of the chloroplast DNA (intron of trnL and intergenic regions of trnT-trnL and trnL-trnF) and ITS1 and ITS2 of the nuclear rDNA. The resulting phylogenetic trees showed polyphyletic relationships among species in the sections Tacamahaca and Aigeiros. Based on chloroplast DNA sequence data, P. nigra had a close affinity to species of section Populus, whereas nuclear DNA sequence data suggested a close relationship between P. nigra and species of the section Aigeiros, suggesting a possible hybrid origin for P. nigra. Similarly, the chloroplast DNA sequences of P. tristis and P. szechuanica were similar to that of the species of section Aigeiros, while the nuclear sequences revealed a close affinity to species of the section Tacamahaca, suggesting a hybrid origin for these two Asiatic balsam poplars. The incongruence between phylogenetic trees based on nuclear- and chloroplast-DNA sequence data suggests a reticulate evolution in the genus Populus.

Summary Wind power is a fast‐growing industry with broad potential to impact volant wildlife. Flight altitude is a key determinant of the risk to wildlife from modern horizontal‐axis wind turbines, which typically have a rotor‐swept zone of 50–150 m above the ground. We used altitudinal GPS data collected from golden eagles Aquila chrysaetos tracked using satellite telemetry to evaluate the potential impacts of wind turbines on eagles and other raptors along migratory routes. Eagle movements during migration were classified as local (1–5 km h −1 ) or migratory (>10 km h −1 ) and were characterized based on the type of terrain over which each bird was flying, and the bird's distance from wind resources preferred for energy development. Birds engaged in local movements turned more frequently and flew at lower altitude than during active migration. This flight behaviour potentially exposes them to greater risk of collision with turbines than when engaged in longer‐distance movements. Eagles flew at relatively lower altitude over steep slopes and cliffs (sites where orographic lift can develop) than over flats and gentle slopes (sites where thermal lift is more likely). Eagles predominantly flew near to wind resources preferred by energy developers, and locally moving eagles flew closer to those wind resources with greater frequency than eagles in active migration. Synthesis and applications . Our research outlines the general effects of topography on raptor flight altitude and demonstrates how topography can interact with raptor migration behaviour to drive a potential human–wildlife conflict resulting from wind energy development. Management of risk to migratory species from industrial‐scale wind turbines should consider the behavioural differences between both locally moving and actively migrating individuals. Additionally, risk assessment for wind energy–wildlife interactions should incorporate the consequences of topography on the flight altitude of potentially impacted wildlife.

Research Article| May 01, 1993 Accretion of Archean oceanic plateau fragments in the Abitibi, greenstone belt, Canada Jean-Philippe Desrochers; Jean-Philippe Desrochers 1Département de Géologie, Université de Montréal, C.P. 6128, Succursale A, Montréal, Québec H3C 3J7, Canada Search for other works by this author on: GSW Google Scholar Claude Hubert; Claude Hubert 1Département de Géologie, Université de Montréal, C.P. 6128, Succursale A, Montréal, Québec H3C 3J7, Canada Search for other works by this author on: GSW Google Scholar John N. Ludden; John N. Ludden 1Département de Géologie, Université de Montréal, C.P. 6128, Succursale A, Montréal, Québec H3C 3J7, Canada Search for other works by this author on: GSW Google Scholar Pierre Pilote Pierre Pilote 2Ministère de l'Énergie et des Ressources du Québec, 400 boulevard Lamaque, Val d'Or, Québec J9P 3L4, Canada Search for other works by this author on: GSW Google Scholar Geology (1993) 21 (5): 451–454. https://doi.org/10.1130/0091-7613(1993)021<0451:AOAOPF>2.3.CO;2 Article history first online: 02 Jun 2017 Cite View This Citation Add to Citation Manager Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Search Site Citation Jean-Philippe Desrochers, Claude Hubert, John N. Ludden, Pierre Pilote; Accretion of Archean oceanic plateau fragments in the Abitibi, greenstone belt, Canada. Geology 1993;; 21 (5): 451–454. doi: https://doi.org/10.1130/0091-7613(1993)021<0451:AOAOPF>2.3.CO;2 Download citation file: Ris (Zotero) Refmanager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentBy SocietyGeology Search Advanced Search Abstract The arc-arc collision model is often applied to the evolution of Archean greenstone belts. This model predicts concordant stratigraphic relations among the various lithologies evolving generally from komatiitic-tholeiitic affinities toward calc-alkalic affinities. New geological data from the Malartic composite block of the Abitibi greenstone belt indicate that this volcanic pile, rather than representing a conformable sequence, is composed of four distinct mafic lithotectonic domains that are overlain by a calc-alkalic sequence. The mafic domains are thought to be examples of accreted Archean oceanic plateau material that were deformed during their collage state. The younger calc-alkalic sequence represents extension-related volcanism that erupted through the ac- creted and deformed plateau material, possibly as a consequence of ridge subduction in a regime of oblique convergence. The relations described here may represent a common feature of Archean terranes. This content is PDF only. Please click on the PDF icon to access. First Page Preview Close Modal You do not have access to this content, please speak to your institutional administrator if you feel you should have access.

White-tailed deer (Odocoileus virginianus) were introduced 100 y ago on Anticosti, a 7,943-km2 island located in the Gulf of St. Lawrence, Québec. The forest of the island is typically boreal and, at the time of the introduction, was dominated by balsam fir (Abies balsamea), white spruce (Picea glauca), and black spruce (P. mariana). Since then, the vegetation has been modified as a result of high deer density (16 deer·km-2) and heavy browsing. The most important change is the progressive decline of balsam fir stands, formerly the prevalent forest type. We describe that process at three spatial and time scales. On the largest scale (whole island, 100 y), we examined the age and species composition of stands on recent forest maps as well as the age structure of 2,555 individual balsam fir and white spruce trees. Balsam fir was largely dominant before 1930, but was replaced by white spruce afterwards. We also estimated that fir stands, which now cover 20% of the island, occupied about 40% of the total area initially. On an intermediate scale (1,200 km2, 30 y), we examined the natural regeneration established after an insect outbreak that took place in 1971-1972 and massively killed predominantly balsam fir forests. In that area, white spruce regeneration now largely replaces that of balsam fir. On a finer scale (< 1 km2, 3 y), we monitored 1,800 balsam fir seedlings (2-5 cm high) individually tagged. Seedlings were browsed throughout the snow-free season, and the annual browsing incidence in forest was estimated at 26%. Considering the present age of remnant fir stands, we estimate that most of these will have been eradicated in 40-50 y.

The evolutionary potential of a species is determined by its genetic diversity. Thus, management plans should integrate genetic concerns into active conservation efforts. The copper redhorse (Moxostoma hubbsi) is an endangered species, with an endemic distribution limited to the Richelieu River and a short section of the St Lawrence River in Quebec, Canada. The population, gradually fragmented since 1849, is characterized by a decline in population size and a lack of recruitment. A total of 269 samples were collected between 1984 and 2004 and genotyped using 22 microsatellite loci, which indicated that these fish comprise a single population, with a global F(ST) value of only 0.0038. Despite a small census size ( approximately 500), a high degree of genetic diversity was observed compared to common values for freshwater fishes (average number of 12.5 alleles/locus and average HO of 0.77 +/- 0.08). No difference was observed between expected and observed pairwise values of relatedness (r(xy): -0.00013 +/- 0.11737), suggesting an outbred population. Long-term Ne was estimated at 4476 whereas contemporary Ne values ranged from 107 to 568, suggesting a pronounced yet gradual demographic decline of the population, as no bottleneck could be detected for the recent past. By means of simulations, we estimated Ne would need to remain at more than approximately 400 to retain 90% of the genetic diversity over 100 years. Overall, these observations corroborate other recent empirical studies confirming that long generation times may act as a buffering effect contributing to a reduction in the pace of genetic diversity erosion in threatened species.

Abstract Logging negatively affects the threatened forest‐dwelling caribou ( Rangifer tarandus caribou ) through its positive effects on large predator populations. As recruitment is a key component of caribou population growth rate, we assessed calving rates of females and calf survival rates during the most critical period for calf survival, the calving period. We also identified causes of calf mortality and investigated the influence of predation risk, food availability, and human disturbance on habitat selection of females during the calving period at both the home‐range and forest stand scales. We hypothesized that caribou should display habitat selection patterns to reduce predation risk at both scales. Using telemetry, we followed 22 females and their calves from 2004 to 2007 in a highly managed study area in Québec, Canada. Most females (78.5 ± 0.05 [SE]) gave birth each year, but only 46.3 ± 8.0% of the calves survived during the first 50 days following birth, and 57.3 ± 14.9% of them died from black bear ( Ursus americanus ) predation. At the home‐range scale, caribou selected calving areas located at upper slope positions and avoided high road density areas. Surprisingly, they also selected the forested habitat type having the lowest lateral cover (mixed and deciduous stands) while avoiding the highest cover (regenerating conifer stands). At the forest stand scale, caribou selected areas located at relatively high elevations and with a lower basal area of black spruce trees. The selection of upper slope positions likely favored spatial segregation between calving females and wolves ( Canis lupus ) but not black bear. Our results suggest that calving females used areas from which they could visually detect approaching predators. While wolf avoidance appeared to be effective in a highly managed landscape, caribou did not appear to have adjusted their predator avoidance strategy to the recent increase in black bear abundance, who have benefited from increased food abundance. This situation requires focused attention from wildlife managers as logging activities are progressing towards the north within the core of forest‐dwelling caribou range. © 2011 The Wildlife Society.

There is general consensus that wildfires in boreal forests will increase throughout this century in response to more severe and frequent drought conditions induced by climate change. However, prediction models generally assume that the vegetation component will remain static over the next few decades. As deciduous species are less flammable than conifer species, it is reasonable to believe that a potential expansion of deciduous species in boreal forests, either occurring naturally or through landscape management, could offset some of the impacts of climate change on the occurrence of boreal wildfires. The objective of this study was to determine the potential of this offsetting effect through a simulation experiment conducted in eastern boreal North America. Predictions of future fire activity were made using multivariate adaptive regression splines (MARS) with fire behavior indices and ecological niche models as predictor variables so as to take into account the effects of changing climate and tree distribution on fire activity. A regional climate model (RCM) was used for predictions of future fire risk conditions. The experiment was conducted under two tree dispersal scenarios: the status quo scenario, in which the distribution of forest types does not differ from the present one, and the unlimited dispersal scenario, which allows forest types to expand their range to fully occupy their climatic niche. Our results show that future warming will create climate conditions that are more prone to fire occurrence. However, unlimited dispersal of southern restricted deciduous species could reduce the impact of climate change on future fire occurrence. Hence, the use of deciduous species could be a good option for an efficient strategic fire mitigation strategy aimed at reducing fire Propagation in coniferous landscapes and increasing public safety in remote populated areas of eastern boreal Canada under climate change.