Ministerio de Defensa

indicated that the reduction of epoxy or hydroxyl groups had a greater impact on the restoration of the conductive nature of the graphite structure in rGO. These findings were reflected in the dramatic change in the structural stability of the rGO nanofoams produced by freeze-drying. The reduction protocol in this study allowed to achieve the highest conductivity values reported so far for the aqueous reduction of graphene oxide mediated by sodium borohydride. The 4-probe sheet resistivity approach used to measure the electrical conductivity is also, for the first time, presented in detail for filtrate sheet assemblies' of stacked GO/rGO sheets.

The Advanced Camera for the Hubble Space Telescope has three cameras. The first, the Wide Field Camera, will be a high- throughput, wide field, 4096 X 4096 pixel CCD optical and I-band camera that is half-critically sampled at 500 nm. The second, the High Resolution Camera (HRC), is a 1024 X 1024 pixel CCD camera that is critically sampled at 500 nm. The HRC has a 26 inch X 29 inch field of view and 29 percent throughput at 250 nm. The HRC optical path includes a coronagraph that will improve the HST contrast near bright objects by a factor of approximately 10 at 900 nm. The third camera, the solar-blind camera, is a far-UV, pulse-counting array that has a relatively high throughput over a 26 inch X 29 inch field of view. The advanced camera for surveys will increase HST's capability for surveys and discovery by a factor of approximately 10 at 800 nm.

Abstract Housing units with closer access to public transportation enjoy a higher market value than those with similar characteristics but poorer access. This difference can be explained by the lower cost of transport to the main workplaces and shopping areas in town. For this reason, investments in public transport infrastructure, such as building a new metro line, are capitalised totally or partially into land and housing prices. This work empirically analyses the degree of capitalisation into housing prices of the benefits of the new Line 4 of the Santiago metro system, which began operating in December 2005. We focus on anticipated capitalisation into housing prices at the moment construction of Line 4 was announced and at the moment information on the basic engineering project was unveiled, identifying the location of the future stations. We use a unique database containing all home buying and selling transactions in the Greater Santiago area between December 2000 and March 2004. The results show that the average apartment price rose by between 4.2 per cent and 7.9 per cent after construction was announced and by between 3.1 per cent and 5.5 per cent after the location of the stations was identified. These increases were not distributed evenly, but depended on the distance from the apartment to the nearest station. An indirect effect of this kind of capitalization is that property tax collections will increase if property is reappraised following the price rise. This effect is not negligible in magnitude and could represent 11 to 17 per cent of investment in the new metro line. This raises and interesting discussion on how the metro network extension is financed.

The association between plant and plant growth promoting bacteria (PGPB) contributes to the successful thriving of plants in extreme environments featured by water shortage. We have recently shown that, with respect to the non-cultivated desert soil, the rhizosphere of pepper plants cultivated under desert farming hosts PGPB communities that are endowed with a large portfolio of PGP traits. Pepper plants exposed to bacterial isolates from plants cultivated under desert farming exhibited a higher tolerance to water shortage, compared with untreated control. This promotion was mediated by a larger root system (up to 40%), stimulated by the bacteria, that enhanced plant ability to uptake water from dry soil. We provide initial evidence that the nature of the interaction can have a limited level of specificity and that PGPB isolates may determine resistance to water stress in plants others than the one of the original isolation. It is apparent that, in relation to plant resistance to water stress, a feature of primary evolutionary importance for all plants, a cross-compatibility between PGPB and different plant models exists at least on a short-term.

The continuous flow synthesis of a series of amines was successfully achieved by exploiting the enhanced stability and broad substrate scope of an immobilised transaminase from <italic>Halomonas elongata</italic> (HEWT).

The basidiomycetes fungi belonging to the genus Pleurotus could make an important contribution to sustainable functional food design because they possess an elevated protein content with a valuable essential amino acid scoring pattern, a unique dietary fibre profile, mainly comprised of branched β-glucan, high levels of some vitamins of the B group, vitamin D, Fe, Zn, Cu, Se and some bioactive mycochemicals, while the Na and fat contents are low. Moreover, Pleurotus spp. can grow efficiently on various clean by-products of food processing, such as wheat straw, wheat stalk and spent beer grain, thus representing a sustainable food source. This review illustrates the compositional variability of Pleurotus spp. grown on various by-products, in order to clarify its potential ability to address the needs of populations with endemic nutritional deficiencies as well as those populations at risk or affected by some chronic diseases. The perspectives for Pleurotus applications in functional foods decisively depend on consumers' acceptability. Hence, the sensory properties of Pleurotus spp. are also clarified herein. Lastly, the three main strategies of functional food development using Pleurotus spp. are summarized, namely its use as a fortifying agent, high-cost protein replacer and prebiotic ingredient.

BACKGROUND: Immune checkpoint therapies (ICTs) targeting the programmed cell death-1 (PD1)/programmed cell death ligand-1 (PD-L1) pathway have improved outcomes for patients with non-small cell lung cancer (NSCLC), particularly those with high PD-L1 expression. However, the predictive value of manual PD-L1 scoring is imperfect and alternative measures are needed. We report an automated image analysis solution to determine the predictive and prognostic values of the product of PD-L1+ cell and CD8+ tumor infiltrating lymphocyte (TIL) densities (CD8xPD-L1 signature) in baseline tumor biopsies. METHODS: Archival or fresh tumor biopsies were analyzed for PD-L1 and CD8 expression by immunohistochemistry. Samples were collected from 163 patients in Study 1108/NCT01693562, a Phase 1/2 trial to evaluate durvalumab across multiple tumor types, including NSCLC, and a separate cohort of 199 non-ICT- patients. Digital images were automatically scored for PD-L1+ and CD8+ cell densities using customized algorithms applied with Developer XD™ 2.7 software. RESULTS: For patients who received durvalumab, median overall survival (OS) was 21.0 months for CD8xPD-L1 signature-positive patients and 7.8 months for signature-negative patients (p = 0.00002). The CD8xPD-L1 signature provided greater stratification of OS than high densities of CD8+ cells, high densities of PD-L1+ cells, or manually assessed tumor cell PD-L1 expression ≥25%. The CD8xPD-L1 signature did not stratify OS in non-ICT patients, although a high density of CD8+ cells was associated with higher median OS (high: 67 months; low: 39.5 months, p = 0.0009) in this group. CONCLUSIONS: An automated CD8xPD-L1 signature may help to identify NSCLC patients with improved response to durvalumab therapy. Our data also support the prognostic value of CD8+ TILS in NSCLC patients who do not receive ICT. TRIAL REGISTRATION: ClinicalTrials.gov identifier: NCT01693562 . Study code: CD-ON-MEDI4736-1108. Interventional study (ongoing but not currently recruiting). Actual study start date: August 29, 2012. Primary completion date: June 23, 2017 (final data collection date for primary outcome measure).

Type-2 diabetes is continuously increasing worldwide. Hence, there is a need to develop functional foods that efficiently alleviate damage due to hyperglycaemia complications while meeting the criteria for a sustainable food processing technology. Inhibition of mammalian α-amylase and α-glucosidase was studied for white grape skin samples recovered from wineries and found to be higher than that of the drug acarbose. In white grape skins, quercetin and kaempferol derivatives, analysed by UPLC-DAD-MS, and the oligomeric series of catechin/epicatechin units and their gallic acid ester derivatives up to nonamers, analysed by MALDI-TOF-MS were identified. White grape skin was then used for enrichment of a tomato puree (3%) and a flat bread (10%). White grape skin phenolics were found in the extract obtained from the enriched foods, except for the higher mass proanthocyanidin oligomers, mainly due to their binding to the matrix and to a lesser extent to heat degradation. Proanthocyanidin solubility was lower in bread, most probably due to formation of binary proanthocyanin/protein complexes, than in tomato puree where possible formation of ternary proanthocyanidin/protein/pectin complexes can enhance solubility. Enzyme inhibition by the enriched foods was significantly higher than for unfortified foods. Hence, this in vitro approach provided a platform to study potential dietary agents to alleviate hyperglycaemia damage and suggested that grape skin phenolics could be effective even if the higher mass proanthocyanidins are bound to the food matrix.

The level of PD-L1 expression in immunohistochemistry (IHC) assays is a key biomarker for the identification of Non-Small-Cell-Lung-Cancer (NSCLC) patients that may respond to anti PD-1/PD-L1 treatments. The quantification of PD-L1 expression currently includes the visual estimation by a pathologist of the percentage (tumor proportional scoring or TPS) of tumor cells showing PD-L1 staining. Known challenges like differences in positivity estimation around clinically relevant cut-offs and sub-optimal quality of samples makes visual scoring tedious and subjective, yielding a scoring variability between pathologists. In this work, we propose a novel deep learning solution that enables the first automated and objective scoring of PD-L1 expression in late stage NSCLC needle biopsies. To account for the low amount of tissue available in biopsy images and to restrict the amount of manual annotations necessary for training, we explore the use of semi-supervised approaches against standard fully supervised methods. We consolidate the manual annotations used for training as well the visual TPS scores used for quantitative evaluation with multiple pathologists. Concordance measures computed on a set of slides unseen during training provide evidence that our automatic scoring method matches visual scoring on the considered dataset while ensuring repeatability and objectivity.

To increase the uptake of biocatalytic processes by industry, it is essential to demonstrate the reliability of enzyme-based methodologies directly applied to the production of high value products.

In the present work, a silver nanoparticle/delphinidin modified glassy carbon electrode (AgNP/Delph/GCE) was fabricated as a highly sensitive electrochemical sensor for gallic acid (GA) determination.

Many biocontrol fluorescent pseudomonads can protect plants from soilborne fungal pathogens through production of the antifungal secondary metabolite 2,4-diacetylphloroglucinol (Phl). One of the phl biosynthetic genes, phlD, encodes a polyketide synthase similar to plant chalcone synthases. Here, restriction analysis of phlD from 39 Phl+ biocontrol fluorescent pseudomonads yielded seven different banding patterns. The gene was sequenced in seven strains, representing the different restriction patterns. Cluster analysis of phlD restriction data or phlD sequences indicated that phlD polymorphism was high, and two main clusters were obtained when predicted PhlD sequences were compared. When the seven PhlD sequences were studied with those of other procaryotic polyketide synthases (gram-positive bacteria) and plant chalcone synthases, however, Phl+ pseudomonads, gram-positive bacteria, and plants clustered separately. Yet, sequence analysis of active site regions for PhlD and plant chalcone synthases revealed that PhlD can be considered a member of the chalcone synthase family, which may be interpreted as convergent evolution of key enzymes involved in secondary metabolism. For the 39 Phl+ pseudomonads, a relationship was found among phlD restriction patterns, phylogenetic groups defined by 16S rDNA restriction analysis (confirmed by 16S rDNA sequencing), and production levels of Phl in vitro.

Modern chemical residues from water pollution (such as herbicides, antibiotics and even chemical substances of military use) are a challenge to survival of microalgal populations. Adaptation of three microalgal species – Pseudanabaena planctonica (Cyanophyceae), Dunaliella tertiolecta (Polyblepharidaceae, Chlorophyceae) and Scenedesmus sp. (Chlorophyceae) – to growth and survival in the presence of erythromycin, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and 2,4,6-trinitrotoluene was analysed as an experimental model. Growth of these species was restricted even by micromolar concentrations of such xenobiotics. When such contaminants are added to a dense microalgal culture, the cell density will be reduced after a few days due to the death of sensitive cells. However, after further incubation for several days, the culture will sometimes increase in density again due to the growth of a cell variant which is resistant to the contaminants. A Luria–Delbrück fluctuation analysis was carried out to distinguish between resistant cells arising from rare spontaneous mutation and resistant cells arising from other mechanisms of adaptation. In all cases, the contaminant-resistant cells arise randomly by rare spontaneous mutation during replication of cells prior to the addition of the contaminant (pre-selective mutations). Since wild-type microalgal genotypes are unable to survive in the presence of such contaminants, spontaneous pre-selective mutation (i.e. mutation from herbicide sensitivity to herbicide resistance) offers insights into the evolutionary capabilities of microalgal populations in contaminated environments. The rate of spontaneous mutation from sensitivity to resistance ranged from 2.0 × 10−6 mutants per cell division for erythromycin sensitive→ erythromycinresistant P. planctonica to 8.2 × 10−6 mutants per cell division for TNTsensitive→ TNTresistant Scenedesmus sp. Since contaminant-resistant mutants have a diminished fitness, the resistant variants are maintained in the absence of the contaminants as the result of balance between new resistant cells arising from spontaneous mutation and resistant cells eliminated by natural selection. Thus recurrence of rare spontaneous pre-selective mutations ensures the survival of microalgal population in suddenly polluted environments.

BACKGROUND: The impact of neuraminidase inhibitors (NAIs) on influenza-related pneumonia (IRP) is not established. Our objective was to investigate the association between NAI treatment and IRP incidence and outcomes in patients hospitalised with A(H1N1)pdm09 virus infection. METHODS: A worldwide meta-analysis of individual participant data from 20 634 hospitalised patients with laboratory-confirmed A(H1N1)pdm09 (n = 20 021) or clinically diagnosed (n = 613) 'pandemic influenza'. The primary outcome was radiologically confirmed IRP. Odds ratios (OR) were estimated using generalised linear mixed modelling, adjusting for NAI treatment propensity, antibiotics and corticosteroids. RESULTS: Of 20 634 included participants, 5978 (29·0%) had IRP; conversely, 3349 (16·2%) had confirmed the absence of radiographic pneumonia (the comparator). Early NAI treatment (within 2 days of symptom onset) versus no NAI was not significantly associated with IRP [adj. OR 0·83 (95% CI 0·64-1·06; P = 0·136)]. Among the 5978 patients with IRP, early NAI treatment versus none did not impact on mortality [adj. OR = 0·72 (0·44-1·17; P = 0·180)] or likelihood of requiring ventilatory support [adj. OR = 1·17 (0·71-1·92; P = 0·537)], but early treatment versus later significantly reduced mortality [adj. OR = 0·70 (0·55-0·88; P = 0·003)] and likelihood of requiring ventilatory support [adj. OR = 0·68 (0·54-0·85; P = 0·001)]. CONCLUSIONS: Early NAI treatment of patients hospitalised with A(H1N1)pdm09 virus infection versus no treatment did not reduce the likelihood of IRP. However, in patients who developed IRP, early NAI treatment versus later reduced the likelihood of mortality and needing ventilatory support.

Strategies to control HIV in the absence of antiretroviral therapy are needed to cure HIV. However, such strategies will require analytical treatment interruptions (ATIs) to determine their efficacy. We investigated how U.S. stakeholders involved in HIV cure research perceive ATIs. We conducted 36 in-depth interviews with three groups of stakeholders: 12 people living with HIV, 11 clinician-researchers, and 13 policy-makers/bioethicists. Qualitative data revealed several themes. First, there was little consensus on when ATIs would be ethically warranted. Second, the most frequent perceived hypothetical motivators for participating in research on ATIs were advancing science and contributing to society. Third, risks related to viral rebound were the most prevalent concerns related to ATIs. Stakeholders suggested ways to minimize the risks of ATIs in HIV cure research. Increased cooperation between scientists and local communities may be useful for minimizing risk. Further ethics research is necessary.

Abstract A simple model for reflection series is desirable for two reasons: first, to have a means of generating model reflection sequences on which to test geophysical data processing techniques, specifically methods of predictive deconvolution, and second, to suggest a way of parameterizing real reflection sequences for classification and further geologic study. In this note we wish to discuss a model suggested by Hosken (1980); our discussion is founded upon the powerful ideas of self-similarity and scaling developed by Mandelbrot (1983) and on the paucity of typical lengths in geology. Typical lengths are common in physics problems; for example, the length of an organ pipe governs the wavelengths of the notes played. When taking photographs, geologists include some manmade object to give scale to the photo. Otherwise it is very hard to tell if, say, folds are of centimeter size or form whole mountains.

In this paper, the impact of considering diffuse multipath components at mm-wave frequencies as well as the significance of selecting appropriate diffuse scattering model parameters is shown. Two different diffuse models, namely, the Lambertian model and the directive model, have been parameterized for several materials typically present in indoor environments. These models are formulated to embed the diffuse scattering phenomenon easily into ray tracing tools. The estimation of the parameters has been performed by comparing measurements and simulations using the models. Once the best fitting parameters have been estimated, they are included in the diffuse components simulation section of a general ray tracing tool. This tool has been used to simulate the power delay profile at 60 GHz in an indoor scenario, including single and double bounce diffuse components. Thanks to the estimated model parameters, the wireless channel at the 60-GHz band can be analyzed, including the diffuse scattering phenomenon, without the need for any previous measurement or simulation. Thus, the channel analysis with ray tracing tools, including dense components, becomes easier, faster, and more reliable.

Residues contribute to water pollution and pose a challenge to microalgal populations because numerous contaminants are toxic to microalgae, even in the micromolar range. Adaptation of microalgae from herbicide sensitivity to herbicide resistance was analysed by an ecological genetic approach, using the unicellular alga Dictyosphaerium chlorelloides (Chlorophyceae) as an experimental model. A dose–effect study showed that the Malthusian parameter under conditions of r selection in an uncrowded environment and the carrying capacity under conditions of K selection in an crowded environment were both restricted even by low concentrations (< 1 μM) of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (DCMU) herbicide. When a culture was treated with 50 μM DCMU, it cleared after a few days, as a result of destruction of sensitive cells by the herbicide. However, after further incubation for several days, the culture sometimes regained colour, owing to the growth of cell variants resistant to the herbicide. A fluctuation analysis was carried out to distinguish between (1) herbicide-resistant cells arising by direct and specific acquired adaptation in response to the herbicide and (2) herbicide-resistant cells arising by rare spontaneous mutations occurring randomly during replication of organisms prior to the incorporation of the herbicide. The fluctuation analysis unequivocally demonstrated that DCMU is not facilitating the development of DCMU-resistant cells; rather, we found that DCMU-resistant cells occur spontaneously by mutation in nonselective conditions prior to the incorporation of the herbicide (preselective mutations). The rate of spontaneous mutation from DCMU sensitivity to DCMU resistance was ∼ 2.2 × 10−6 mutants per cell division. Mutation was recurrent from a normal wild-type DCMU-sensitive allele to an DCMU-resistant allele, but such herbicide-resistant alleles were detrimental in terms of fitness in the absence of the herbicide. A competition experiment between wild-type DCMU-sensitive cells and DCMU-resistant mutants showed that, in small populations, the DCMU-resistant mutants are driven to extinction. The resistant variants are maintained in the absence of the herbicide as the result of a balance between new resistant cells arising by rare spontaneous mutation and resistant cells eliminated by natural selection. In our case, the average frequency of DCMU-resistant mutants in the absence of the DCMU is about five DCMU-resistant mutants per million cells. The results of our experimental model suggest that spontaneous mutation from herbicide sensitivity to herbicide resistance is sufficient in itself to assure the survival of microalgae populations in herbicide-contaminated environments when the population size is large.

Modern chemical residues from water pollution (such as herbicides, antibiotics and even chemical substances of military use) are a challenge to survival of microalgal populations. Adaptation of three microalgal species – Pseudanabaena planctonica (Cyanophyceae), Dunaliella tertiolecta (Polyblepharidaceae, Chlorophyceae) and Scenedesmus sp. (Chlorophyceae) – to growth and survival in the presence of erythromycin, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and 2,4,6-trinitrotoluene was analysed as an experimental model. Growth of these species was restricted even by micromolar concentrations of such xenobiotics. When such contaminants are added to a dense microalgal culture, the cell density will be reduced after a few days due to the death of sensitive cells. However, after further incubation for several days, the culture will sometimes increase in density again due to the growth of a cell variant which is resistant to the contaminants. A Luria–Delbrück fluctuation analysis was carried out to distinguish between resistant cells arising from rare spontaneous mutation and resistant cells arising from other mechanisms of adaptation. In all cases, the contaminant-resistant cells arise randomly by rare spontaneous mutation during replication of cells prior to the addition of the contaminant (pre-selective mutations). Since wild-type microalgal genotypes are unable to survive in the presence of such contaminants, spontaneous pre-selective mutation (i.e. mutation from herbicide sensitivity to herbicide resistance) offers insights into the evolutionary capabilities of microalgal populations in contaminated environments. The rate of spontaneous mutation from sensitivity to resistance ranged from 2.0 × 10−6 mutants per cell division for erythromycin sensitive→ erythromycinresistant P. planctonica to 8.2 × 10−6 mutants per cell division for TNTsensitive→ TNTresistant Scenedesmus sp. Since contaminant-resistant mutants have a diminished fitness, the resistant variants are maintained in the absence of the contaminants as the result of balance between new resistant cells arising from spontaneous mutation and resistant cells eliminated by natural selection. Thus recurrence of rare spontaneous pre-selective mutations ensures the survival of microalgal population in suddenly polluted environments.

High quality graphene has been obtained by polysaccharide-assisted ultrasonication in aqueous medium. This approach provides an economical, solvent-free, high-yield, and industrially scalable route for new applications of graphene-based nanocomposites.