Montana State University Billings

BACKGROUND: The advent and use of highly sensitive molecular biology techniques to explore the microbiota and microbiome in environmental and tissue samples have detected the presence of contaminating microbial DNA within reagents. These microbial DNA contaminants may distort taxonomic distributions and relative frequencies in microbial datasets, as well as contribute to erroneous interpretations and identifications. RESULTS: We herein report on the occurrence of bacterial DNA contamination within commonly used DNA extraction kits and PCR reagents and the effect of these contaminates on data interpretation. When compared to previous reports, we identified an additional 88 bacterial genera as potential contaminants of molecular biology grade reagents, bringing the total number of known contaminating microbes to 181 genera. Many of the contaminants detected are considered normal inhabitants of the human gastrointestinal tract and the environment and are often indistinguishable from those genuinely present in the sample. CONCLUSIONS: Laboratories working on bacterial populations need to define contaminants present in all extraction kits and reagents used in the processing of DNA. Any unusual and/or unexpected findings need to be viewed as possible contamination as opposed to unique findings.

This article reviews research on counterfactual, social, and temporal comparisons and proposes a Reflection and Evaluation Model (REM) as an organizing framework. At the heart of the model is the assertion that 2 psychologically distinct modes of mental simulation operate during comparative thinking: reflection, an experiential ("as if") mode of thinking characterized by vividly simulating that information about the comparison standard is true of, or part of, the self; and evaluation, an evaluative mode of thinking characterized by the use of information about the standard as a reference point against which to evaluate one's present standing. Reflection occurs when information about the standard is included in one's self-construal, and evaluation occurs when such information is excluded. The result of reflection is that standard-consistent cognitions about the self become highly accessible, thereby yielding affective assimilation; whereas the result of evaluation is that comparison information is used as a standard against which one's present standing is evaluated, thereby yielding affective contrast. The resulting affect leads to either an increase or decrease in behavioral persistence as a function of the type of task with which one is engaged, and a combination of comparison-derived causal inferences and regulatory focus strategies direct one toward adopting specific future action plans.

Biogeochemical models must include a broad variety of biological and physical processes to test our understanding of the terrestrial carbon cycle and to predict ecosystem biomass and carbon fluxes. We combine the photosynthesis and biophysical calculations in the Simple Biosphere model, Version 2.5 (SiB2.5) with the biogeochemistry from the Carnegie‐Ames‐Stanford Approach (CASA) model to create SiBCASA, a hybrid capable of estimating terrestrial carbon fluxes and biomass from diurnal to decadal timescales. We add dynamic allocation of Gross Primary Productivity to the growth and maintenance of leaves, roots, and wood and explicit calculation of autotrophic respiration. We prescribe leaf biomass using Leaf Area Index (LAI) derived from remotely sensed Normalized Difference Vegetation Index. Simulated carbon fluxes and biomass are consistent with observations at selected eddy covariance flux towers in the AmeriFlux network. Major sources of error include the steady state assumption for initial pool sizes, the input weather data, and biases in the LAI.

ABSTRACT The Devonian Sanish pool of the Antelope field has several unusual characteristics which make it almost unique in the Williston basin. Some of these are: (1) high productivity of several wells from a nebulous, ill-defined reservoir; (2) association with the steepest dip in the central part of the basin; (3) very high initial reservoir pressure; and (4) almost complete absence of water production. Analysis of these factors indicates that Sanish productivity is a function of tension fracturing associated with the relatively sharp Antelope structure. Fracture porosity and fracture permeability can be related mathematically to bed thickness and structural curvature (the second derivative of structure). It is found that fracture porosity varies directly as the product of bed thickness times curvature and that fracture permeability varies as the third power of this product. A map of structural curvature in the Sanish pool shows good coincidence between areas of maximum curvature and areas of best productivity. Volumetric considerations show that the quantities of oil being produced cannot be coming from the Sanish zone. It is concluded that the overlying, very petroliferous Bakken Shale is the immediate, as well as the ultimate, source of this oil. The role of the Sanish fracture system is primarily that of a gathering system for many increments of production from the Bakken. The extremely high initial reservoir pressure indicates that the Sanish-Bakken accumulation is in an isolated, completely oil-saturated reservoir and, hence, is independent of structure in the normal sense. Similar accumulations should be present anywhere in the Williston basin where a permeable bed, of limited areal extent, is in direct contact with either of the two Bakken shale beds.

Since Crohn's disease is a transmural disease, we hypothesized that examination of deep submucosal tissues directly involved in the inflammatory disease process may provide unique insights into bacterial populations transgressing intestinal barriers and bacterial populations more representative of the causes and agents of the disease. We performed deep 16s microbiota sequencing on isolated ilea mucosal and submucosal tissues on 20 patients with Crohn's disease and 15 non-inflammatory bowel disease controls with a depth of coverage averaging 81,500 sequences in each of the 70 DNA samples yielding an overall resolution down to 0.0001% of the bacterial population. Of the 4,802,328 total sequences generated, 98.9% or 4,749,183 sequences aligned with the Kingdom Bacteria that clustered into 8545 unique sequences with <3% divergence or operational taxonomic units enabling the identification of 401 genera and 698 tentative bacterial species. There were significant differences in all taxonomic levels between the submucosal microbiota in Crohn's disease compared to controls, including organisms of the Order Desulfovibrionales that were present within the submucosal tissues of most Crohn's disease patients but absent in the control group. A variety of organisms of the Phylum Firmicutes were increased in the subjacent submucosa as compared to the parallel mucosal tissue including Ruminococcus spp., Oscillospira spp., Pseudobutyrivibrio spp., and Tumebacillus spp. In addition, Propionibacterium spp. and Cloacibacterium spp. were increased as well as large increases in Proteobacteria including Parasutterella spp. and Methylobacterium spp. This is the first study to examine the microbial populations within submucosal tissues of patients with Crohn's disease and to compare microbial communities found deep within the submucosal tissues with those present on mucosal surfaces. Our data demonstrate the existence of a distinct submucosal microbiome and ecosystem that is not well reflected in the mucosa and/or downstream fecal material.

Phylogenetic relationships among Pinus species from subgenus Strobus remain unresolved despite combined efforts based on nrITS and cpDNA. To provide greater resolution among these taxa, a 900-bp intron from a late embryogenesis abundant (LEA)-like gene (IFG8612)was sequenced from 39 pine species, with two or more alleles representing 33 species. Nineteen of 33 species exhibited allelic nonmonphyly in the strict consensus tree, and 10 deviated significantly from allelic monophyly based on topology incongruence tests. Intraspecific nucleotide diversity ranged from 0.0 to 0.0211, and analysis of variance shows that nucleotide diversity was strongly associated (P < 0.0001)with the degree of species monophyly. Although species nonmonophyly complicates phylogenetic interpretations, this nuclear locus offers greater topological support than previously observed for cpDNA or nrITS. Lacking evidence for hybridization, recombination, or imperfect taxonomy, we feel that incomplete lineage sorting remains the best explanation for the polymorphisms shared among species. Depending on the species, coalescent expectations indicate that reciprocal monophyly will be more likely than paraphyly in 1.71 to 24.0 x 10(6) years, and that complete genome-wide coalescence in these species may require up to 76.3 x 10(6) years. The absence of allelic coalescence is a severe constraint in the application of phylogenetic methods in Pinus, and taxa sharing similar life history traits with Pinus are likely to show species nonmonophyly using nuclear markers.

(1998). The People's Lobby: Organizational Innovation and the Rise of Interest Group Politics in the United States, 1890–1925. History: Reviews of New Books: Vol. 26, No. 3, pp. 119-119.

Urban legend has often characterized frequent emergency department (ED) patients as mentally ill substance users who are a costly drain on the health care system and who contribute to ED overcrowding because of unnecessary visits for conditions that could be treated more efficiently elsewhere. This study of Medicaid ED users in New York City shows that behavioral health conditions are responsible for a small share of ED visits by frequent users, and that ED use accounts for a small portion of these patients' total Medicaid costs. Frequent ED users have a substantial burden of disease, and they have high rates of primary and specialty care use. They also have linkages to outpatient care that are comparable to those of other ED patients. It is possible to use predictive modeling to identify who will become a repeat ED user and thus to help target interventions. However, policy makers should view reducing frequent ED use as only one element of more-comprehensive intervention strategies for frequent health system users.

In this era of curriculum reconstruction, considerable attention is being focused on curriculum integration. The integration of science and mathematics continues to be interpreted in different ways. In this article, five different meanings of integration of science and mathematics–discipline specific, content specific, process, methodological and thematic–are investigated along with instructional implications of these different approaches to integration.

OBJECTIVE: To evaluate the interleukin-1 inhibitor rilonacept (Interleukin-1 Trap) for prevention of gout flares occurring in the first few months following initiation of urate-lowering therapy. METHODS: In this double-blind study, adult patients with hyperuricemia and gout were randomized to receive rilonacept administered subcutaneously once per week (loading dose 320 mg followed by 160 mg weekly) or placebo, and started on allopurinol (300 mg/day, titrated to serum urate <6 mg/dl). At study visits, physical and laboratory assessments were performed and information on any adverse events was ascertained. RESULTS: Baseline characteristics were similar between the rilonacept and placebo groups (n = 41 and n = 42, respectively). The mean number of gout flares per patient through week 12 (primary efficacy end point) was markedly lower in the rilonacept group than in the placebo group (0.15 [6 flares] versus 0.79 [33 flares]; P = 0.0011). Fewer flares were observed with rilonacept as early as 4 weeks after initiation of treatment (P = 0.007). The proportion of patients experiencing a flare during the 12 weeks was lower in the rilonacept group than in the placebo group (14.6% versus 45.2%; P = 0.0037). No rebound in the flare rate was observed for 6 weeks after discontinuation of rilonacept or placebo at week 16. Adverse events were similar between groups, and no deaths or serious infectious adverse events were reported; the most common adverse events were infections (14.6% and 26.2% of rilonacept- and placebo-treated patients, respectively) and musculoskeletal disorders (14.6% and 21.4%, respectively). A higher percentage of rilonacept-treated patients (98%) compared with placebo-treated patients (79%) completed the primary 12-week evaluation period (P = 0.015). CONCLUSION: The current findings indicate that rilonacept significantly reduces the frequency of gout flares during the initial period of treatment with urate-lowering therapy, with a favorable safety profile.

Asperger's Syndrome is a pervasive developmental disorder characterized by deficits in social comnmniunication and by repetitive patterns of behaviors or interests. It is observed in some ifted children. The author proposes that gifted children with Asperger's Syndromie mnay not be identified because their unusual behaviors may be wrongly attributed to either their giftedness or to a learning disability. The article discusses ways in which Asperger's Syndrome might be missed in gifted children and proposes guidelines for differentiating characteristics of giftedness fromn characteristics of Asperger's Syndrone.

In research-practice partnerships (RPPs), the line between researcher and practitioner can be blurred, and the roles for everyone involved may be unclear. Yet little is known about how these roles are negotiated and with what consequences for collaborative efforts. Guided by organizational theory, we share findings from a multiyear case study of one RPP, drawing on observations of partnership leadership meetings and interviews with school district leaders and partners. Role negotiation occurred in more than one third of leadership meetings, as evidenced by identity-referencing discourse. When roles were unclear, collaborative efforts stalled; once partners renegotiated their roles, it changed how they engaged in the work together. Several forces contributed to these dynamics, including the partner’s ambitious yet ambiguous identity and the introduction of new members to the group. This study offers implications for those engaged in partnership work and provides a foundation for future research regarding role negotiation in RPPs.

Abstract A vast and growing interdisciplinary research effort has focused on the rise of the so‐called New West, purportedly the product of regional socioeconomic, political, and ecological upheavals in states like Montana and Colorado. Reviewing the growing research on this problem in sociology, economics, geography, and conservation science, this article identifies four central questions at the core of this diverse scholarship. Our review demonstrates that none of these central questions has generated consensus conclusions and that there is untapped potential for more structurally robust analyses of the drivers and outcomes of rapid change in the region. Indeed, supporting other analyses that have called the consistency of the region into question, our survey suggests the ways in which this region is not unique, but largely reflective of larger scale socioecological forces playing out in similar ways around the postindustrial world. We conclude, therefore, with a series of crucial questions, which may be unanswerable by assuming the “New West” as a coherent geography.

Since its discovery in the 1980s, the fatty acid hydroxylase flavocytochrome P450 (cytochrome P450) BM3 (CYP102A1) from Bacillus megaterium has been adopted as a paradigm for the understanding of structure and mechanism in the P450 superfamily of enzymes. P450 BM3 was the first P450 discovered as a fusion to its redox partner--a eukaryotic-like diflavin reductase. This fact fuelled the interest in soluble P450 BM3 as a model for the mammalian hepatic P450 enzymes, which operate a similar electron transport chain using separate, membrane-embedded P450 and reductase enzymes. Structures of each of the component domains of P450 BM3 have now been resolved and detailed protein engineering and molecular enzymology studies have established roles for several amino acids in, e.g. substrate binding, coenzyme selectivity and catalysis. The potential of P450 BM3 for biotechnological applications has also been recognized, with variants capable of industrially important transformations generated using rational mutagenesis and forced evolution techniques. This paper focuses on recent developments in our understanding of structure and mechanism of this important enzyme and highlights important problems still to be resolved.

Urban redevelopment governances are commonly treated as singular, monolithic entities that are interactively homogeneous, deploying uniform ensembles of policies and practices across their respective cities. This study, alternatively, reveals these formations as adroitly proactive and interactively heterogeneous across their respective cities. Through a racial economy lens, we empirically examine the racial contours of this “governance heterogeneity” in one urban setting: Chicago, Illinois. In this frame, a comparative analysis of Chicago’s Bronzeville and Pilsen neighborhoods is presented. Both neighborhoods are constituted by different racial profiles: Bronzeville is home to a predominantly African-American population, whereas Pilsen is mostly Mexican and Mexican-American. The study reveals that redevelopment governances are differentially responsive to established, deeply rooted racialized conceptions of “Blackness” and “Latinoness.” As a result, the form and trajectory of redevelopment in both settings has unfolded in markedly different ways.

A research project was conducted to analyze student achievement using submitted assignments for two sections of a graduate course in human development and learning, taught both online and face-to-face, as well as three sections of undergraduate educational psychology, two of which were taught face-to-face, and one taught online. Results suggest there were no significant differences between the work submitted by students from the online sections and from the face-to-face students, and that the methods of instruction are more important than the delivery platform.

Candidatus Chloracidobacterium thermophilum, which naturally inhabits microbial mats of alkaline siliceous hot springs in Yellowstone National Park, is the only known chlorophototroph in the phylum Acidobacteria. The Ca. C. thermophilum genome was composed of two chromosomes (2,683,362 bp and 1,012,010 bp), and both encoded essential genes. The genome included genes to produce chlorosomes, the Fenna-Matthews-Olson protein, bacteriochlorophylls a and c as principal pigments, and type-1, homodimeric reaction centres. Ca. C. thermophilum is an aerobic photoheterotroph that lacks the ability to synthesize several essential nutrients. Key genes of all known carbon fixation pathways were absent, as were genes for assimilatory nitrate and sulfate reduction and vitamin B(12) synthesis. Genes for the synthesis of branched-chain amino acids (valine, isoleucine and leucine) were also absent, but genes for catabolism of these compounds were present. This observation suggested that Ca. C. thermophilum may synthesize branched-chain amino acids from an intermediate(s) of the catabolic pathway by reversing these reactions. The genome encoded an aerobic respiratory electron transport chain that included NADH dehydrogenase, alternative complex III and cytochrome oxidase. The chromosomes of the laboratory isolate were compared with assembled, metagenomic scaffolds from the major Ca. C. thermophilum population in hot-spring mats. The larger chromosomes of the two populations were highly syntenous but significantly divergent (~13%) in sequence. In contrast, the smaller chromosomes have undergone numerous rearrangements, contained many transposases, and might be less constrained by purifying selection than the large chromosomes. Some transposases were homologous to those of mat community members from other phyla.

ABSTRACT An individual's training status is a key factor used to determine the volume, the intensity, and the selection of exercises for resistance training prescription. Interestingly, there are no objective parameters to assess training status, so there is ambiguity in determining the appropriate volume and other resistance training variables in this regard. Thus, the objective of this study was to propose a strategy for classification and determination of resistance training status. The following five parameters were identified and used: (a) current uninterrupted training time, (b) time of detraining, (c) previous training experience, (d) exercise technique, and (e) strength level. Moreover, 4 classification levels are proposed: beginner, intermediate, advanced, and highly advanced, which are determined by the mean score of the parameters used. The proposed model represents an important advancement in training status classification and can be used as a valid tool for training prescription and for researchers to better characterize a sample and reproduce results under the same conditions in future studies.

Three studies examined the motivational implications of thinking about how things could have been worse. It was hypothesized that when these downward counterfactuals yield negative affect, through consideration of the possibility of a negative outcome, motivation to change and improve would be increased (the wake-up call). When downward counterfactuals yield positive affect, through diminishing the impact of a potentially negative outcome, motivation to change and improve should be reduced (the Pangloss effect). Results from three studies supported these hypotheses. Studies 1 and 2 showed that a manipulation of the counterfactual made about an investment influenced decisions toward that investment. Study 3 showed that students’ academic motivation was influenced by a manipulation of the type of downward counterfactual they made after an exam and that affect mediated the relationship between the counterfactual and motivation.

An Internet presence is a critical early component in the process of building towards a fully operational and unified e‐commerce strategy. E‐commerce has significantly impacted logistics/supply chain strategies and the development and implementation of a website have become key issues for many firms within the transportation industry. This study provides an overview of website content within the motor carrier industry. Content analysis was used to assess the site design, informational content, and the interactive content of 152 motor carrier websites, and to compare the features offered on the Top 100 motor carrier firm websites with those offered on the sites of smaller carrier firms.