Nagaoka University

The detection methods and generation mechanisms of the intrinsic reactive oxygen species (ROS), i.e., superoxide anion radical (•O2–), hydrogen peroxide (H2O2), singlet oxygen (1O2), and hydroxyl radical (•OH) in photocatalysis, were surveyed comprehensively. Consequently, the major photocatalyst used in heterogeneous photocatalytic systems was found to be TiO2. However, besides TiO2 some representative photocatalysts were also involved in the discussion. Among the various issues we focused on the detection methods and generation reactions of ROS in the aqueous suspensions of photocatalysts. On the careful account of the experimental results presented so far, we proposed the following apprehension: adsorbed •OH could be regarded as trapped holes, which are involved in a rapid adsorption–desorption equilibrium at the TiO2–solution interface. Because the equilibrium shifts to the adsorption side, trapped holes must be actually the dominant oxidation species whereas •OH in solution would exert the reactivity mainly for nonadsorbed reactants. The most probable routes of generating intrinsic ROS at the surfaces of two polymorphs of TiO2, anatase and rutile, were discussed along with some plausible rational reaction processes. In addition to the four major ROS, three ROS, that is organic peroxides, ozone, and nitric oxide, which are less common in photocatalysis are also briefly reviewed.

New quick-response and high-efficiency control of an induction motor, which is quite different from that of the field-oriented control is proposed. The most obvious differences between the two are as follows. 1) The proposed scheme is based on limit cycle control of both flux and torque using optimum PWM output voltage; a switching table is employed for selecting the optimum inverter output voltage vectors so as to attain as fast a torque response, as low an inverter switching frequency, and as low harmonic losses as possible. 2) The efficiency optimization in the steady-state operation is also considered; it can be achieved by controlling the amplitude of the flux in accordance with the torque command. To verify the feasibility of this scheme, experimentation, simulation, and comparison with field-oriented control are carried out. The results prove the excellent characteristics for torque response and efficiency, which confirm the validity of this control scheme.

The conventional reactive power in single-phase or three- phase circuits has been defined on the basis of the average value concept for sinusoidal voltage and current waveforms in steady states. The instantaneous reactive power in three-phase circuits is defined on the basis of the instantaneous value concept for arbitrary voltage and current waveforms, including transient states. A new instantaneous reactive power compensator comprising switching devices is proposed which requires practically no energy storage components.

We report on the population properties of compact binary mergers inferred from gravitational-wave observations of these systems during the first three LIGO-Virgo observing runs. The Gravitational-Wave Transient Catalog 3 (GWTC-3) contains signals consistent with three classes of binary mergers: binary black hole, binary neutron star, and neutron star–black hole mergers. We infer the binary neutron star merger rate to be between 10 and <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" display="inline"><a:mrow><a:mn>1700</a:mn><a:mtext> </a:mtext><a:mtext> </a:mtext><a:msup><a:mrow><a:mi>Gpc</a:mi></a:mrow><a:mrow><a:mo>−</a:mo><a:mn>3</a:mn></a:mrow></a:msup><a:mtext> </a:mtext><a:msup><a:mrow><a:mi>yr</a:mi></a:mrow><a:mrow><a:mo>−</a:mo><a:mn>1</a:mn></a:mrow></a:msup></a:mrow></a:math> and the neutron star–black hole merger rate to be between 7.8 and <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" display="inline"><c:mrow><c:mn>140</c:mn><c:mtext> </c:mtext><c:mtext> </c:mtext><c:msup><c:mrow><c:mi>Gpc</c:mi></c:mrow><c:mrow><c:mo>−</c:mo><c:mn>3</c:mn></c:mrow></c:msup><c:mtext> </c:mtext><c:msup><c:mrow><c:mi>yr</c:mi></c:mrow><c:mrow><c:mo>−</c:mo><c:mn>1</c:mn></c:mrow></c:msup></c:mrow></c:math>, assuming a constant rate density in the comoving frame and taking the union of 90% credible intervals for methods used in this work. We infer the binary black hole merger rate, allowing for evolution with redshift, to be between 17.9 and <e:math xmlns:e="http://www.w3.org/1998/Math/MathML" display="inline"><e:mrow><e:mn>44</e:mn><e:mtext> </e:mtext><e:mtext> </e:mtext><e:msup><e:mrow><e:mi>Gpc</e:mi></e:mrow><e:mrow><e:mo>−</e:mo><e:mn>3</e:mn></e:mrow></e:msup><e:mtext> </e:mtext><e:msup><e:mrow><e:mi>yr</e:mi></e:mrow><e:mrow><e:mo>−</e:mo><e:mn>1</e:mn></e:mrow></e:msup></e:mrow></e:math> at a fiducial redshift (<g:math xmlns:g="http://www.w3.org/1998/Math/MathML" display="inline"><g:mi>z</g:mi><g:mo>=</g:mo><g:mn>0.2</g:mn></g:math>). The rate of binary black hole mergers is observed to increase with redshift at a rate proportional to <i:math xmlns:i="http://www.w3.org/1998/Math/MathML" display="inline"><i:mo stretchy="false">(</i:mo><i:mn>1</i:mn><i:mo>+</i:mo><i:mi>z</i:mi><i:msup><i:mo stretchy="false">)</i:mo><i:mi>κ</i:mi></i:msup></i:math> with <m:math xmlns:m="http://www.w3.org/1998/Math/MathML" display="inline"><m:mi>κ</m:mi><m:mo>=</m:mo><m:mn>2.</m:mn><m:msubsup><m:mn>9</m:mn><m:mrow><m:mo>−</m:mo><m:mn>1.8</m:mn></m:mrow><m:mrow><m:mo>+</m:mo><m:mn>1.7</m:mn></m:mrow></m:msubsup></m:math> for <o:math xmlns:o="http://www.w3.org/1998/Math/MathML" display="inline"><o:mi>z</o:mi><o:mo>≲</o:mo><o:mn>1</o:mn></o:math>. Using both binary neutron star and neutron star–black hole binaries, we obtain a broad, relatively flat neutron star mass distribution extending from <q:math xmlns:q="http://www.w3.org/1998/Math/MathML" display="inline"><q:msubsup><q:mn>1.2</q:mn><q:mrow><q:mo>−</q:mo><q:mn>0.2</q:mn></q:mrow><q:mrow><q:mo>+</q:mo><q:mn>0.1</q:mn></q:mrow></q:msubsup></q:math> to <s:math xmlns:s="http://www.w3.org/1998/Math/MathML" display="inline"><s:msubsup><s:mn>2.0</s:mn><s:mrow><s:mo>−</s:mo><s:mn>0.3</s:mn></s:mrow><s:mrow><s:mo>+</s:mo><s:mn>0.3</s:mn></s:mrow></s:msubsup><s:msub><s:mi>M</s:mi><s:mo stretchy="false">⊙</s:mo></s:msub></s:math>. We confidently determine that the merger rate as a function of mass sharply declines after the expected maximum neutron star mass, but cannot yet confirm or rule out the existence of a lower mass gap between neutron stars and black holes. We also find the binary black hole mass distribution has localized over- and underdensities relative to a power-law distribution, with peaks emerging at chirp masses of <v:math xmlns:v="http://www.w3.org/1998/Math/MathML" display="inline"><v:msubsup><v:mn>8.3</v:mn><v:mrow><v:mo>−</v:mo><v:mn>0.5</v:mn></v:mrow><v:mrow><v:mo>+</v:mo><v:mn>0.3</v:mn></v:mrow></v:msubsup></v:math> and <x:math xmlns:x="http://www.w3.org/1998/Math/MathML" display="inline"><x:msubsup><x:mn>27.9</x:mn><x:mrow><x:mo>−</x:mo><x:mn>1.8</x:mn></x:mrow><x:mrow><x:mo>+</x:mo><x:mn>1.9</x:mn></x:mrow></x:msubsup><x:msub><x:mi>M</x:mi><x:mo stretchy="false">⊙</x:mo></x:msub></x:math>. While we continue to find that the mass distribution of a binary’s more massive component strongly decreases as a function of primary mass, we observe no evidence of a strongly suppressed merger rate above approximately <ab:math xmlns:ab="http://www.w3.org/1998/Math/MathML" display="inline"><ab:mn>60</ab:mn><ab:msub><ab:mi>M</ab:mi><ab:mo stretchy="false">⊙</ab:mo></ab:msub></ab:math>, which would indicate the presence of a upper mass gap. Observed black hole spins are small, with half of spin magnitudes below <db:math xmlns:db="http://www.w3.org/1998/Math/MathML" display="inline"><db:msub><db:mi>χ</db:mi><db:mi>i</db:mi></db:msub><db:mo>≈</db:mo><db:mn>0.25</db:mn></db:math>. While the majority of spins are preferentially aligned with the orbital angular momentum, we infer evidence of antialigned spins among the binary population. We observe an increase in spin magnitude for systems with more unequal-mass ratio. We also observe evidence of misalignment of spins relative to the orbital angular momentum. Published by the American Physical Society 2023

The control strategy of active power filters using switching devices is proposed on the basis of the instantaneous reactive power theory. This aims at excellent compensation characteristics in transient states as well as steady states. The active power filter is developed, of which the power circuit consists of quadruple voltage-source PWM converters. As the result, interesting compensation characteristics were verified experimentally which could not be obtained by the active power filter based on the conventional reactive power theory.

A novel approach to compensating for harmonics in power systems is presented. It is a combined system of a shunt passive filter and a small rated series active filter. The compensation principle is described, and some filtering characteristics are discussed in detail. Excellent practicability and validity to compensate for harmonics in power systems are demonstrated experimentally. Although the source harmonic voltage was only 1%, the source harmonic current reached about 10% before the series active filter was started. After it was started, no harmonic current flowed into the shunt passive filter. In addition, no harmonic voltage appeared at the terminals of the shunt passive filter, because the source harmonic voltage was applied to the series active filter. The total loss of the series active filter was less than 40 W. It is concluded that the combined system is far superior in efficiency to conventional shunt active filters.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">&gt;</ETX>

This paper proposes a novel control strategy of a PWM converter with no power source voltage sensors. The strategy has two features to improve a total power factor including harmonic components without detecting the voltages. One is an estimation technique of the power source voltages, which can estimate instantaneous values of the voltages by evaluating instantaneous active and reactive power according to every switching mode of the converter. The other is a direct instantaneous power control technique, which can directly control the instantaneous active and reactive power by using switching modes as manipulated values for the converter. A DSP based experimental system was developed, and experimental tests were conducted. The control period of the system was only 15 /spl mu/s. It was confirmed that the maximum total power factor was more than 99%, and the maximum efficiency was 96.2%. The results have proven excellent performance of the proposed system.

Approximate layer-averaged equations describing the mechanics of turbid underflows are derived. Closure of the equations describing the balance of fluid mass, sediment mass, and mean flow momentum provides for the delineation of a three-equation model. A description of sediment exchange with the bed allows for the possibility of a self-accelerating turbidity current in which sediment entrainment from the bed is linked to flow velocity. A consideration of the balance of the mean energy of the turbulence yields a constraint on physically realistic solutions to the three-equation model. It is shown that the self-acceleration predicted by the three-equation model is so strong that the energy constraint fails to be satisfied. In particular, the turbulent energy consumed in entraining new bed sediment exceeds the supply of energy to the turbulence, so that the turbulence, and thus the turbidity current, must die. The problem is rectified by the formulation of a four-equation model, in which an explicit accounting is made of the mean energy of the turbulence. Sediment entrainment from the bed is linked to the level of turbulence in the four-equation model. Self-acceleration is again predicted, although it is somewhat subdued compared with that predicted by the three-equation model. The predictions of both models are summarized over a wide range of conditions.

A unified approach to obtain the characteristics of almost-periodic grating slab waveguides including gain in the waveguide is reported. In this approach the waveguides are divided into short segments, and in each segment the gratings are assumed to be periodic, that is, parameters such as coupling coefficient, grating phase, deviations from the Bragg frequency, and gain in the waveguide are independent of a propagation direction z. Then characteristics of almost-periodic grating slab waveguides can be obtained by multiplying each F matrix of a short segment with the proper grating phase conditions at the interface between two adjacent segments. The appropriateness of this approach is shown for typical aperiodic grating waveguides such as tapered, chirped, and phase-shifted gratings. The results obtained by this method are compared with others and prove to be in good agreement with the results obtained by other methods. In addition to these characteristics, it is shown that the F matrix can be used to obtain the threshold conditions for distributed feedback laser oscillations including reflections from cleaved edges.

Lignins are the most abundant aromatic compounds in nature, and their decomposition is essential to the terrestrial carbon cycle. White rot fungi secreting phenol oxidases are assumed to be involved in the initial degradation of native lignin, whereas bacteria play a main role in the mineralization of lignin-derived low-molecular-weight compounds in soil. There are a number of reports on the degradation pathways for lignin-derived aromatic compounds, but their catabolism has not been enzymatically or genetically characterized. Sphingomonas paucimobilis SYK-6 is one of the best-characterized lignin-degrading bacteria. It can grow on a wide variety of lignin-related biaryls and monoaryls, including beta-aryl ether, biphenyl, diarylpropane, and phenylpropane. These compounds are degraded via the protocatechuate (PCA) 4,5-cleavage pathway or multiple 3-O-methylgallate (3MGA) catabolic pathways. In this review, the enzyme systems for beta-aryl ether and biphenyl degradation, O demethylation linked with one carbon metabolism, the PCA 4,5-cleavage pathway, and the multiple 3MGA catabolic pathways in SYK-6 are outlined.

The recent detections of gravitational waves (GWs) reported by LIGO/Virgo collaborations have made significant impact on physics and astronomy. A global network of GW detectors will play a key role to solve the unknown nature of the sources in coordinated observations with astronomical telescopes and detectors. Here we introduce KAGRA (former name LCGT; Large-scale Cryogenic Gravitational wave Telescope), a new GW detector with two 3-km baseline arms arranged in the shape of an "L", located inside the Mt. Ikenoyama, Kamioka, Gifu, Japan. KAGRA's design is similar to those of the second generations such as Advanced LIGO/Virgo, but it will be operating at the cryogenic temperature with sapphire mirrors. This low temperature feature is advantageous for improving the sensitivity around 100 Hz and is considered as an important feature for the third generation GW detector concept (e.g. Einstein Telescope of Europe or Cosmic Explorer of USA). Hence, KAGRA is often called as a 2.5 generation GW detector based on laser interferometry. The installation and commissioning of KAGRA is underway and its cryogenic systems have been successfully tested in May, 2018. KAGRA's first observation run is scheduled in late 2019, aiming to join the third observation run (O3) of the advanced LIGO/Virgo network. In this work, we describe a brief history of KAGRA and highlights of main feature. We also discuss the prospects of GW observation with KAGRA in the era of O3. When operating along with the existing GW detectors, KAGRA will be helpful to locate a GW source more accurately and to determine the source parameters with higher precision, providing information for follow-up observations of a GW trigger candidate.

We report results of a search for an isotropic gravitational-wave background (GWB) using data from Advanced LIGO's and Advanced Virgo's third observing run (O3) combined with upper limits from the earlier O1 and O2 runs. Unlike in previous observing runs in the advanced detector era, we include Virgo in the search for the GWB. The results of the search are consistent with uncorrelated noise, and therefore we place upper limits on the strength of the GWB. We find that the dimensionless energy density GW 5.8 10 -9 at the 95% credible level for a flat (frequency-independent) GWB, using a prior which is uniform in the log of the strength of the GWB, with 99% of the sensitivity coming from the band 20-76.6 Hz; GW f 3.4 10 -9 at 25 Hz for a power-law GWB with a spectral index of 2=3 (consistent with expectations for compact binary coalescences), in the band 20-90.6 Hz; and GW f 3.9 10 -10 at 25 Hz for a spectral index of 3, in the band 20-291.6 Hz. These upper limits improve over our previous results by a factor of 6.0 for a flat GWB, 8.8 for a spectral index of 2=3, and 13.1 for a spectral index of 3. We also search for a GWB arising from scalar and vector modes, which are predicted by alternative theories of gravity; we do not find evidence of these, and place upper limits on the strength of GWBs with these polarizations. We demonstrate that there is no evidence of correlated noise of magnetic origin by performing a Bayesian analysis that allows for the presence of both a GWB and an effective magnetic background arising from geophysical Schumann resonances. We compare our upper limits to a fiducial model for the GWB from the merger of compact binaries, updating the model to use the most recent datadriven population inference from the systems detected during O3a. Finally, we combine our results with observations of individual mergers and show that, at design sensitivity, this joint approach may yield stronger constraints on the merger rate of binary black holes at z 2 than can be achieved with individually resolved mergers alone.

Experiments were conducted to determine the behaviour of turbidity currents laden with non-cohesive silt (silica flour) moving down a slope the bed of which is covered with similar silt. Each current was sustained with constant inlet conditions for six to eight minutes. The motion of the head was not studied; measurements were concentrated on the continuous part of the current that was essentially constant in time but developing in space. Only supercritical currents were studied. The currents were free to erode sediment from, and deposit sediment on, the bed. Measurements of vertical profiles of downstream velocity and sediment concentration allowed for the development of approximate similarity relations. These relations can be used to estimate most of the shape factors that occur in the vertically-integrated equations of motion. The values do not deviate grossly from those found by using a "top-hat" function, i.e. a step function, such that velocity or concentration equals its average value in the layer and vanishes outside the layer. Coefficients of water entrainment from above, sediment entrainment from the bed, and bed resistance were determined. Where possible, the data were compared with other data and relations in the literature. Several modified relations were developed. Several researchers have suggested that a continuous supercritical turbidity current would ultimately either "ignite" (self-accelerate and entrain sediment in the net) or subside (decelerate and deposit sediment in the net) in the downstream direction. This ignition hypothesis has been offered as an explanation for the formation of submarine canyons. The experimental facility was too short to allow for a test of ignition. If the data obtained are used in conjunction with the equations of motion to extrapolate the experimentally measured currents farther downstream, however, ignition is predicted in several cases.

Glass-ceramics are noted for their unusual combination of properties and manifold commercialized products for consumer and specialized markets. Evolution of novel glass and ceramic processing routes, a plethora of new compositions, and unique exotic nano- and microstructures over the past 60 years led us to review the definition of glass-ceramics. Well-established and emerging processing methods, such as co-firing, additive manufacturing, and laser patterning are analyzed concerning the core requirements of processing glass-ceramics and the performance of the final products. In this communication, we propose a revised, updated definition of glass-ceramics, which reads “Glass-ceramics are inorganic, non-metallic materials prepared by controlled crystallization of glasses via different processing methods. They contain at least one type of functional crystalline phase and a residual glass. The volume fraction crystallized may vary from ppm to almost 100%”.

The physical and photocatalytic properties of a novel solid solution between GaN and ZnO, (Ga(1-x)Zn(x))(N(1-x)O(x)), are investigated. Nitridation of a mixture of Ga(2)O(3) and ZnO at 1123 K for 5-30 h under NH(3) flow results in the formation of a (Ga(1-x)Zn(x))(N(1-x)O(x)) solid solution with x = 0.05-0.22. With increasing nitridation time, the zinc and oxygen concentrations decrease due to reduction of ZnO and volatilization of zinc, and the crystallinity and band gap energy of the product increase. The highest activity for overall water splitting is obtained for (Ga(1-x)Zn(x))(N(1-x)O(x)) with x = 0.12 after nitridation for 15 h. The crystallinity of the catalyst is also found to increase with increasing the ratio of ZnO to Ga(2)O(3) in the starting material, resulting in an increase in activity.

The present article introduces VLC for automotive applications using an image sensor. In particular, V2I-VLC and V2V-VLC are presented. While previous studies have documented the effectiveness of V2I and V2V communication using radio technology in terms of improving automotive safety, in the present article, we identify characteristics unique to image-sensor-based VLC as compared to radio wave technology. The two primary advantages of a VLC system are its line-of-sight feature and an image sensor that not only provides VLC functions, but also the potential vehicle safety applications made possible by image and video processing. Herein, we present two ongoing image-sensor-based V2I-VLC and V2VVLC projects. In the first, a transmitter using an LED array (which is assumed to be an LED traffic light) and a receiver using a high-framerate CMOS image sensor camera is introduced as a potential V2I-VLC system. For this system, real-time transmission of the audio signal has been confirmed through a field trial. In the second project, we introduce a newly developed CMOS image sensor capable of receiving highspeed optical signals and demonstrate its effectiveness through a V2V communication field trial. In experiments, due to the high-speed signal reception capability of the camera receiver using the developed image sensor, a data transmission rate of 10 Mb/s has been achieved, and image (320 × 240, color) reception has been confirmed together with simultaneous reception of various internal vehicle data, such as vehicle ID and speed.

Lignin is the most abundant phenolic polymer; thus, its decomposition by microorganisms is fundamental to carbon cycling on earth. Lignin breakdown is initiated by depolymerization catalysed by extracellular oxidoreductases secreted by white-rot basidiomycetous fungi. On the other hand, bacteria play a predominant role in the mineralization of lignin-derived heterogeneous low-molecular-weight aromatic compounds. The outline of bacterial catabolic pathways for lignin-derived bi- and monoaryls are typically composed of the following sequential steps: (i) funnelling of a wide variety of lignin-derived aromatics into vanillate and syringate, (ii) O demethylation of vanillate and syringate to form catecholic derivatives and (iii) aromatic ring-cleavage of the catecholic derivatives to produce tricarboxylic acid cycle intermediates. Knowledge regarding bacterial catabolic systems for lignin-derived aromatic compounds is not only important for understanding the terrestrial carbon cycle but also valuable for promoting the shift to a low-carbon economy via biological lignin valorisation. This review summarizes recent progress in bacterial catabolic systems for lignin-derived aromatic compounds, including newly identified catabolic pathways and genes for decomposition of lignin-derived biaryls, transcriptional regulation and substrate uptake systems. Recent omics approaches on catabolism of lignin-derived aromatic compounds are also described.

The microbial diversity of two types of methanogenic granular sludge, mesophilic (35 degrees C) and thermophilic (55 degrees C), which had been treating sucrose/propionate/acetate-based artificial wastewater were compared. 16S rDNA clone libraries were constructed by PCR with a prokaryote-specific primer set, and partial sequencing of the clonal 16S rDNAs was conducted for phylogenetic analysis. Of 115 mesophilic granule and 110 thermophilic granule clones sequenced, 19 and 22%, respectively, were phylogenetically affiliated with the domain Archaea, and the remainder in each case were assigned to the domain Bacteria. Within the domain Archaea, the 16S rDNA clones in both libraries showed relatively close relationships with those of methanogens. Within the Bacteria, a major group represented in the mesophilic clone library was the delta subclass of the Proteobacteria (27%), in which high degrees of relatedness were observed between the clonal 16S rDNA sequences and those of previously identified syntrophic bacteria and sulfate-reducing bacteria. In contrast, in the thermophilic clone library, the Thermodesulfovibrio group (19%), the green non-sulfur bacteria (18%) and the low G + C subclass of the Gram-positive bacteria (18%) were predominant. A significant difference between the two libraries was that no clone affiliated with the Proteobacteria was detected in the thermophilic clone library, whereas the Proteobacteria was detected in the thermophilic clone library, whereas the Proteobacteria was the most predominant group in the mesophilic clones. Thirty-six and 24 different sequences were found in the mesophilic and thermophilic clones, respectively, suggesting that the microbial diversity of the thermophilic granule was lower than that of the mesophilic granule.

OBJECTIVE: The purpose of this study was to determine the factors influencing diagnostic accuracy in CT-guided automated needle biopsies of lung nodules. SUBJECTS AND METHODS: One hundred thirty-eight consecutive CT-guided automated needle biopsy procedures were performed in 123 patients (124 pulmonary nodules). Factors for diagnostic accuracy were evaluated through analysis of the procedures, which were classified into a success group (true-positive and true-negative) and a failure group (false-positive and false-negative). RESULTS: Final diagnoses were 81 malignant lesions (91 biopsies) and 43 benign lesions (47 biopsies). More than two CT-guided biopsies were performed for 13 lesions. Seventy lesions were true-positive, 44 were true-negative, three were false-positive, and 21 were false-negative. The overall diagnostic accuracy was 82.6%. The sensitivity for malignancy and specificity for benign lesions were 76.9% and 93.6%, respectively. Positive and negative predictive values were 95.9% and 67.7%, respectively. Lesion size was a significant factor contributing to diagnostic accuracy (p = 0.014). Mean diameters of lesions (+/-SD) in the success and failure groups were 24.1+/-12.4 mm and 17.6+/-7.8 mm, respectively. For lesions 6-10 mm in diameter, diagnostic accuracy was 66.7%; for lesions 11-20 mm in diameter, 78.9%; for lesions 21-30 mm in diameter, 86.7%; for lesions 31-50 mm in diameter, 93.3%; and for lesions 51-70 mm in diameter, 100%. CONCLUSION: Lesion size was a determining factor in diagnostic accuracy. Diagnostic accuracy decreased in proportion to the decrease in the lesion diameter.

We have isolated a new recessive dwarf mutant of rice (Oryza sativa L. cv Nipponbare). Under normal growth conditions, the mutant has very short leaf sheaths; has short, curled, and frizzled leaf blades; has few tillers; and is sterile. Longitudinal sections of the leaf sheaths revealed that the cell length along the longitudinal axis is reduced, which explains the short leaf sheaths. Transverse sections of the leaf blades revealed enlargement of the motor cells along the dorsal-ventral axis, which explains the curled and frizzled leaf blades. In addition, the number of crown roots was smaller and the growth of branch roots was weaker than those in the wild-type plant. Because exogenously supplied brassinolide considerably restored the normal phenotypes, we designated the mutant brassinosteroid-dependent 1 (brd1). Further, under darkness, brd1 showed constitutive photomorphogenesis. Quantitative analyses of endogenous sterols and brassinosteroids (BRs) indicated that BR-6-oxidase, a BR biosynthesis enzyme, would be defective. In fact, a 0.2-kb deletion was detected in the genomic region of OsBR6ox (a rice BR-6-oxidase gene) in the brd1 mutant. These results indicate that BRs are involved in many morphological and physiological processes in rice, including the elongation and unrolling of leaves, development of tillers, skotomorphogenesis, root differentiation, and reproductive growth, and that the defect of BR-6-oxidase caused the brd1 phenotype.