National Institute for Educational Policy Research

Intraspecific variation is a major component of biodiversity, yet it has received relatively little attention from governmental and nongovernmental organizations, especially with regard to conservation plans and the management of wild species. This omission is ill-advised because phenotypic and genetic variations within and among populations can have dramatic effects on ecological and evolutionary processes, including responses to environmental change, the maintenance of species diversity, and ecological stability and resilience. At the same time, environmental changes associated with many human activities, such as land use and climate change, have dramatic and often negative impacts on intraspecific variation. We argue for the need for local, regional, and global programs to monitor intraspecific genetic variation. We suggest that such monitoring should include two main strategies: (i) intensive monitoring of multiple types of genetic variation in selected species and (ii) broad-brush modeling for representative species for predicting changes in variation as a function of changes in population size and range extent. Overall, we call for collaborative efforts to initiate the urgently needed monitoring of intraspecific variation.

A large plasmid-encoded protein, VirG, on the bacterial surface is essential for the spreading of Shigella by eliciting polar deposition of filamentous actin in the cytoplasm of epithelial cells. VirG expression from the large plasmid is diminished greatly when it is introduced into Escherichia coli K-12 from Shigella. In an attempt to identify factors affecting VirG expression, we found that the absence of the ompT gene, encoding outer membrane protease OmpT, restored full production of VirG protein to E. coli K-12. Conversely, upon introduction of the ompT gene of E. coli K-12 into Shigella, spreading ability was completely abolished, probably because of the proteolytic degradation of VirG protein by OmpT. Analysis of the DNA sequence of the ompT region indicated that the absence of the ompT gene occurred in Shigella and enteroinvasive E. coli strains, and that the absent DNA segment corresponded to a remnant lambdoid phage structure found in E. coli K-12, which encompasses a 21 kb DNA segment spanning from argU through to the ompT genes. Since ompT is located near purE in E. coli K-12 and a virulence locus for provoking keratoconjunctivitis in the eyes of guinea-pigs, named kcpA, is located near purE in S. flexneri, and the two loci are involved in VirG expression, the KcpA- mutants of S. flexneri 2a constructed were examined for correlation between acquisition of ompT and VirG degradation. Our data suggest that the previous recognition of a kcpA locus in S. flexneri is the result of transfer of the ompT gene from E. coli K-12, giving rise to a KcpA- phenotype. These results indicate that the lack of OmpT protease confers upon Shigella the ability to spread into adjacent epithelial cells.

Seven virulence loci have been identified by Tn5 insertion mutagenesis on the large 230 kb plasmid (pMYSH6000) of Shigella flexneri 2a. In this study, we used Tn10 insertion mutagenesis and identified a novel virulence locus on pMYSH6000 responsible for bacterial spread. Characterization of the invading bacteria of the Tn10 insertion mutants in the epithelial cells revealed that the bacteria were capable of at least some intracellular spreading but not intercellular spreading. Immunoblot analysis of lysates of the Tn10 insertion mutants with a VirG-specific antipeptide antibody revealed diminished levels of the 116 kDa VirG protein. The virG mRNA in the mutants, however, was expressed at the same level as that in the wild type. The DNA region required for the virulence phenotype was localized to a 1.6 kb DNA sequence in the SalI-K fragment on the plasmid, and thus the locus was designated virK. Expression of virK in Escherichia coli using a T7 RNA polymerase-dependent promoter system yielded a 36 kDa protein. The nucleotide sequence of 1642 bp encoding VirK function was determined, and an open reading frame encoding 316 amino acid residues was shown to encode the VirK protein. The virK region was highly conserved among the large virulence plasmids of shigellae and enteroinvasive Escherichia coli. These results suggest that VirK function is an essential virulence determinant for shigellae involved in the expression of virG gene product at post-transcriptional level.

The oculo-motor re ects the viewer s ability to process visual information. This paper examines whether the oculo-motor was affected by two factors: rstly task dif culty and secondly eye-movement frequency. In this paper, oculo-motor indices were de ned as measurements of pupil size, blink and eye-movement. For the purpose of this study, two experiments were designed based on previous subsequential ocular tasks were subjects were required to solve a series of mathematical problems and to orally report their calculations.The results of this experiment found that pupil size and blink rate increased in response to task dif culty in the oral calculation group. In contrast however both the saccade occurrence rate and saccade length were found to decrease with the increased dif culty of the task. The results suggests that oculo-motor indices respond to task dif culty. Secondly, eye-movement frequencies were elicited by the switching frequency of a visual target. Pupil size and the saccade time were found to increase with the frequency however, blink and gazing time were found to decrease in response to the frequency. There was a negative correlation between blinking and gazing time. Additionally, the correlation between blinking and saccade time appeared in the higher frequencies.These results indicate the oculo-motor indices are affected by both task dif culty and eye-movement frequency. Furthermore, eye-movement frequency appears to play a different role than that of task dif culty.

Treatment plans of carbon-ion radiotherapy have been made on the assumption that the beams are delivered instantaneously irrespective to the dose delivery time as well as the interruption time. The advanced therapeutic techniques such as a hypofractionation and a respiratory gating usually require more time to deliver a fractioned dose than conventional techniques. The purpose of this study was to investigate the effects of dose-delivery time structure on biological effectiveness in carbon-ion radiotherapy. The rate equations defined in the microdosimetric kinetic model (MKM) for primary lesions caused in the DNA were reanalyzed and applied to continuous or interrupted irradiation with therapeutic carbon-ion beams. The rate constants characterizing the time of the primary nonlethal lesions to repair or to convert to lethal lesion were experimentally determined for human salivary gland (HSG) tumor cells. Treatment plans were made for a patient case on the assumption that the beam is delivered instantaneously. The RBE weighted absorbed doses of 2.65, 3.45 and 6.86 Gy (RBE) was prescribed to the target. These plans were recalculated by varying the dose delivery time and the interruption time ranging from 1-60 min based on the MKM with the determined parameters. The sum of rate constants for nonlethal lesion to repair a and to convert to lethal lesion c, (a + c), is 2.19 ± 0.40 h⁻¹. The biological effectiveness in the target decreases with the dose delivery time T in continuous irradiation compared to the planned one due to the repair of nonlethal lesions during the irradiation. The biological effectiveness in terms of equivalent acute dose decreases to 99.7% and 96.4% for T = 3 and 60 min in 2.65 Gy (RBE), 99.5% and 94.3% in 4.35 Gy (RBE), and 99.4% and 91.7% in 6.86 Gy (RBE), respectively. For all the cases, the decrease of biological effectiveness is larger at the proximal side with low-LET than the distal side with high-LET. Similar reductions of biological effectiveness with comparable amounts are observed in the interrupted irradiations with prolonged interruption time τ. For the fraction time, i.e., T and/or τ, shorter than 3 min, the decrease of the biological effectiveness with respect to the planned one is less than 1.0%. However, if the fraction time prolongs to 30 min or longer, the biological effectiveness is significantly influenced in carbon-ion radiotherapy, especially with high-prescribed doses. These effects, if confirmed by clinical studies, should be considered in designing the carbon-ion treatment planning.

Oral administration of mycobacterial 65-kDa heat shock protein (HSP) given daily for 5 days prior to immunization with Mycobacterium tuberculosis (Mt) suppressed the development of adjuvant arthritis (AA) in rats. AA was significantly suppressed by 30 and 300 micrograms HSP, and variably by 0.3, 3 micrograms or 1 mg. Histological analysis of joint samples obtained from control and test rats confirmed the suppression of AA in the fed group. Feeding Mt or hen egg lysozyme (HEL) failed to affect AA, indicating that the suppression was HSP specific. The oral administration of 30 micrograms HSP decreased both delayed-type hypersensitivity (DTH) reactions and proliferative responses to HSP and Mt. In addition, the proliferation of lymph node cells (LNC) from Mt-sensitized rats was inhibited by the addition of spleen cells (SPC) from HSP-fed animals, possibly by the secretion of transforming growth factor (TGF)-beta. Spleen cells obtained from tolerized donors were capable of transferring the tolerance to naive recipients. These results demonstrate that feeding HSP is an effective way to suppress AA and that the suppression of AA may be mediated by regulatory T cells generated following oral administration of mycobacterial 65-kDa HSP.

Socioeconomic status (SES) has a powerful influence on cognitive, social and brain development. Children from low-SES backgrounds show poor executive function (EF). However, it is unclear if there is a SES-dependent disparity in functional brain development. The present study examined whether the SES of preschool children (N = 93) is associated with prefrontal activation during cognitive shifting tasks as measured by near-infrared spectroscopy. Low-SES children did not show activation in lateral prefrontal regions during the tasks, whereas middle- and high-SES children showed prefrontal activations, although no differences were found in terms of behavioural performance. These results suggest that SES can affect the functional development of the prefrontal regions. In this study, we discuss the practical implications of the results.

We developed a novel gelatin particle agglutination test (MLPA) for the serodiagnosis of leprosy; this test is especially useful for clinical practice and epidemiological surveys of leprosy in countries in which the disease is endemic. The antigen used in the test is the chemically synthesized trisaccharide moiety of Mycobacterium leprae-specific phenolic glycolipid I. MLPA is a simple and easy technique having sensitivity and specificity comparable to those of the conventional indirect enzyme-linked immunosorbent assay. The new technique was found to be useful for monitoring of chemotherapy and predictive diagnosis of high-risk individuals in contact with persons with leprosy and may be useful for the prediction of relapse. We are now preparing to supply a quality-controlled ready-to-use MLPA kit for leprosy control in countries in which leprosy is endemic.

Purpose The purpose of this paper is to clarify the lesson study (LS) processes and evaluate their effectiveness in Japanese elementary and secondary (middle and high) schools, through a school survey by the National Institute for Educational Policy Research of Japan (NIER). Design/methodology/approach The authors randomly selected 1,000 elementary schools and 1,000 middle schools and 500 high schools in Japan. Survey items are methods of LS, and indicators of building professional learning communities (PLC) through LSs, close communication between teachers, high quality instruction by teachers, and test scores of students of the school. Findings Based on the school survey in elementary and middle schools, almost all schools set up a school‐wide committee, a research theme, and a schedule for LS, and LSs were implemented as part of a school‐wide lesson study from which an action research report is produced. On the other hand, in high schools, almost all the schools implemented LSs, but each LS is independent and implemented specifically for the professional development of the individual teacher who undertakes the research lesson. The authors consider LS as a way to facilitate a PLC in the school. There are correlations between the methods of LS and the indicators of a PLC in elementary and middle schools. However, the effectiveness of LS differs between elementary and middle schools. With respect to the research theme and the organization and discussion of lesson plans, LS methods in middle schools are developed into LS methods in elementary schools. The LS methods may be developed gradually both in elementary and middle schools. Originality/value Statistical data of LS in Japanese elementary and secondary schools are presented for the first time, demonstrating the effectiveness of LS.

Responding to international concerns regarding childhood bullying and a need to identify a common bullying measure, this study examines the comparability of children's self-reports of bullying across five countries. The Pacific-Rim Bullying Measure, a self-report measure of students' experiences with six different types of bullying behaviour and victimization, was administered to 1,398 Grade 5 students from Australia, Canada, Japan, Korea, and United States. Multigroup confirmatory factor analysis and item response theory modeling were used to evaluate construct equivalence on the measure across different countries. Preliminary results revealed some construct differences across countries, that is, the bullying measure is measuring one construct, but that the construct is manifested differently in the different countries.

Abstract This paper investigates teleworker efficiency in Japan in the COVID-19 era by using unique survey data on telework. Many Japanese workers started teleworking during the pandemic and found both benefits as well as impediments. Overall, we find that telework experience and the work environment (e.g., having clearly specified tasks and a flexible working system), changes in work–life balance (e.g., working time), and good mental health improves teleworker efficiency.

Kitagawa F. (2007) The regionalization of science and innovation governance in Japan?, Regional Studies 41, 1099–1114. Traditionally a country that has favoured the centralized national direction of industry–science relationships, Japan is gradually transforming its innovation system with the ‘regionalization’ of science and technology (S&T) policies, characterized by the promotion of ‘clusters’ and academic entrepreneurship. However, there remain fundamental questions as to the development and actual potential of truly ‘regional’ governance structures to guide science policy in target areas. This paper examines developments in the governance of science and innovation in Japan, with a particular focus on the policy instruments used, their impact on regional economic development, organizational embeddedness in regions and the ability of regions to coordinate innovation support policies. Kitagawa F. (2007) La régionalisation de la gouvernance de la science et de l'innovation au Japon?, Regional Studies 41, 1099–1114. Traditionnellement un pays qui a favorisé le dirigisme quant aux rapports entre la science et l'innovation, le Japon transforme progressivement son système d'innovation par la ‘régionalisation’ de ses politiques en faveur de la science et de la technologie (S et T), ce qui se caractérise par la promotion de ‘grappes’ et de l'esprit d'entreprise académique. Cependant, il reste des questions fondamentales quant au développement et au potentiel réel des structures de gouvernance vraiment ‘régionales’ susceptibles de piloter la politique en faveur de la science dans des zones cibles. Cet article cherche à examiner des développements dans le domaine de la gouvernance de la science et de l'innovation au Japon, portant notamment sur les outils de politique employés, leur impact sur le développement économique régional, l'ancrage organisationnel régional et la capacité des régions à coordonner des politiques de soutien pour l'innovation. Innovation Politique de S et T Régionalisation Gouvernance Esprit d'entreprise académique Japon Kitagawa F. (2007) Regionalisierung der Regierungsführung für Wissenschaft und Innovation in Japan?, Regional Studies 41, 1099–1114. Bisher war Japan ein Land, das auf eine zentralisierte nationale Leitung der Beziehungen zwischen Industrie und Wissenschaft setzte. Jetzt wandelt es sein Innovations, system allmählich um, indem es die Politiken für Wissenschaft und Technologie ,regionalisiert', was durch die Förderung von ,Clustern' und von akademischem Unternehmertum gekennzeichnet ist. Es verbleiben jedoch grundlegende Fragen hinsichtlich der Entwicklung und des tatsächlichen Potenzials für wirklich ,regionale' Strukturen der Regierungsführung zur Leitung der Wissenschaftspolitik in den Zielbereichen. In diesem Beitrag werden die Entwicklungen in der Regierungsführung für Wissenschaft und Innovation in Japan untersucht, wobei besonders auf die eingesetzten politischen Instrumente, ihre Auswirkung auf die regionale Wirtschaftsentwicklung, die organisationelle Einbettung in die Regionen und die Fähigkeit der Regionen zur Koordination der Politiken zur Unterstützung der Innovation eingegangen wird. Innovation Wissenschafts-und Technologiepolitik Regionalisierung Regierungsführung Akademisches Unternehmertum Japan Kitagawa F. (2007) ¿La regionalización de la gobernanza de la ciencia y la innovación en Japón?, Regional Studies 41, 1099–1114. Un país como Japón que tradicionalmente ha favorecido la dirección nacional centralizada de las relaciones entre la industria y la ciencia, está ahora transformando poco a poco su sistema de innovación con la regionalización de las políticas científicas y tecnológicas, caracterizadas por el fomento de agrupaciones y el empresariado académico. Sin embargo, quedan por responder cuestiones fundamentales en cuanto al desarrollo y el potencial actual de verdaderas estructuras de gobernanza regional para guiar la política científica en áreas importantes. En este artículo examino los desarrollos en la gobernanza de la ciencia y la innovación en Japón prestando especial atención a qué instrumentos políticos se utilizan, cómo repercuten en el desarrollo económico regional y cómo se integran en las regiones a nivel organizativo. Asimismo analizo cuál es la capacidad de las regiones para coordinar las políticas de apoyo a la innovación. Innovación Política científica y tecnológica Regionalización Gobernanza Empresariado académico Japón

In order to verify the participation of some cytokines in the expression of the suppressor activity of splenic macrophages (M phi s) induced by Mycobacterium avium complex (MAC) infection, we studied whether anticytokine antibodies were capable of blocking their suppressor activity against concanavalin A (ConA)-induced mitogenesis of splenocytes (SPCs). When either anti-tumor necrosis factor (TNF), anti-transforming growth factor-beta (TGF-beta), or anti-interferon-gamma (IFN-gamma) antibody was added to culture medium, suppressor activity was markedly reduced, in the order of anti-TNF, anti-IFN-gamma, and anti-TGF-beta antibodies. By contrast, neither anti-interleukin-6 (IL-6) nor anti-IL-10 antibody exerted such a blocking effect. Therefore, TNF, IFN-gamma, and TGF-beta seem to be related to the full display of the suppressor function of MAC-induced M phi s. However, TNF-alpha and IFN-gamma but not TGF-beta were substantially lacking in inhibitory action against SPC mitogenesis, when added exogenously. Hence, it is unlikely that TNF-alpha and INF-gamma directly modulated the proliferative response of T cells. On the other hand, both TNF-alpha and IFN-gamma potentiated the effector function of the suppressor M phi s. Because their suppressor activity was severely reduced by NG-monomethyl-L-arginine and aminoguanidine, nitric oxide (NO) synthase inhibitors, an NO-dependent mechanism is important for the expression of the immunosuppressive function of MAC-induced M phi s. Moreover, because these M phi s seem to produce a substantial amount of TNF-alpha in membrane-bound form, cell-to-cell contact might be needed for efficient expression of their suppressor action on target T cells.

article Share on Making a place for seniors on the Net: SeniorNet, senior identity, and the digital divide Authors: Mizuko Ito National Institute for Educational Policy Research of Japan, Tokyo, Japan National Institute for Educational Policy Research of Japan, Tokyo, JapanView Profile , Vicki L. O'Day Univ. of California, Santa Cruz, Santa Cruz, CA Univ. of California, Santa Cruz, Santa Cruz, CAView Profile , Annette Adler Agilent Labs, Palo Alto, CA Agilent Labs, Palo Alto, CAView Profile , Charlotte Linde NASA Ames Research Center, Moffett Field, CA NASA Ames Research Center, Moffett Field, CAView Profile , Elizabeth D. Mynatt Georgia Institute of Techology, Altanta Georgia Institute of Techology, AltantaView Profile Authors Info & Claims ACM SIGCAS Computers and SocietyVolume 31Issue 3September 2001 pp 15–21https://doi.org/10.1145/504696.504699Published:01 September 2001Publication History 23citation1,297DownloadsMetricsTotal Citations23Total Downloads1,297Last 12 Months31Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access

One widely accepted explanation of bullying, known as the aggressive-motive thesis, assumes that bullying is a form of aggressive behaviour triggered by external stress. However, recent evidences have suggested a different explanation, known as the frustration-aggression thesis, which asserts that bullying is a psychological defense triggered by external stress to reduce anxiety. The present investigation is an attempt to compare school bullying in Japan and Hong Kong and to find out whether the frustration-aggression thesis is applicable in the schooling context of both societies. Data analysis using structural equation modeling was based on 703 girls selected from 13 primary and 6 junior secondary schools in Japan and 2,477 girls selected from 21 primary and 19 secondary schools in Hong Kong. Results of the analysis suggest that girl bullying in Japan can be explained by the frustration-aggression thesis but that girl bullying in Hong Kong can be explained by the aggressive-motive thesis. The reasons behind the different bullying mechanisms are suggested, but further investigations are needed to uncover the underlying causes.</br>[Copyright of Educational Research & Evaluation is the property of Routledge. Full article may be available at the publisher's website: </br>http://dx.doi.org/10.1080/13803610701702894]

As artificial intelligence systems increasingly make high-stakes recommendations and decisions automatically in many facets of our lives, the use of explainable artificial intelligence to inform stakeholders about the reasons behind such systems has been gaining much attention in a wide range of fields, including education. Also, in the field of education there has been a long history of research into self-explanation, where students explain the process of their answers. This has been recognized as a beneficial intervention to promote metacognitive skills, however, there is also unexplored potential to gain insight into the problems that learners experience due to inadequate prerequisite knowledge and skills that are required, or in the process of their application to the task at hand. While this aspect of self-explanation has been of interest to teachers, there is little research into the use of such information to inform educational AI systems. In this paper, we propose a system in which both students and the AI system explain to each other their reasons behind decisions that were made, such as: self-explanation of student cognition during the answering process, and explanation of recommendations based on internal mechanizes and other abstract representations of model algorithms.

Low executive function (EF) during early childhood is a major risk factor for developmental delay, academic failure, and social withdrawal. Susceptible genes may affect the molecular and biological mechanisms underpinning EF. More specifically, genes associated with the regulation of prefrontal dopamine may modulate the response of prefrontal neurons during executive control. Several studies with adults and older children have shown that variants of the catechol-O-methyltransferase (COMT) gene are associated with behavioral performance and prefrontal activations in EF tasks. However, the effect of the COMT genotype on prefrontal activations during EF tasks on young children is still unknown. The present study examined whether a common functional polymorphism (Val158Met) in the COMT gene was associated with prefrontal activations and cognitive shifting in 3- to 6-year-old children. The study revealed that, compared with children with at least one Met allele (Met/Met and Met/Val), children who were Val homozygous (i) were more able to flexibly switch rules in cognitive shifting tasks and (ii) exhibited increased activations in lateral prefrontal regions during these tasks. This is the first evidence that demonstrates the relationship between a gene polymorphism and prefrontal activations in young children. It also indicates that COMT Val homozygosity may be advantageous for cognitive shifting and prefrontal functions, at least during early childhood, and children who possess this variant may have a lower risk of developing future cognitive and social development issues.

BACKGROUNDS: Austerity has been shown to have an adverse influence on people's mental health and suicide rates. Most existing studies have focused on the governments' reactions to a single event, for example, the Great Recession of 2008. METHODS: This study focused on significant changes in fiscal policy between 2001 and 2014 in Japan. The size of expenditures by national and local governments decreased dramatically between 2001 and 2006 under the neoliberal reform and then increased after the global economic crisis and the Great East Japan Earthquake. Using the data from 47 prefectures between 2001 and 2014, we tested whether more spending by the local governments was associated with a lower suicide rate in their jurisdiction. We also investigated whether this relationship was particularly salient during a more severe recession. RESULTS: Our analysis revealed that an increase of 1% in the per capita local government expenditures was associated with a decrease of 0.2% in the suicide rates among males and females aged between 40 and 64 and that this correlation was strengthened as the unemployment rate increased, particularly among males. CONCLUSIONS: Government's reaction to economic crises can either exacerbate or mitigate the negative impact of the economic recession on people's mental health and suicide rates.

We examine the influence of task types on information-seeking behaviors on the Web by using screen capture logs and eye-movement data. Eleven participants performed two different types of web search, an informational task and a transactional task, and their think aloud protocols and behaviors were recorded. Analyses of the screen capture logs showed that the task type affected the participants' informationseeking behaviors. In the transactional task, participants visited more web pages than for the informational task, but their reading time for each page was shorter than in the informational task. A preliminary analysis of eye-movement data for nine participants revealed characteristics of the scanpaths followed in search result pages as well as the distribution of lookzones for each task.

This paper investigates whether birth weight itself causes individuals’ future life chances. By using a sample of twins in Japan and controlling for the potential effects of genes and family backgrounds, we examine the effect of birth weight on later educational and economic outcomes. The most important finding is that birth weight has a causal effect on academic achievement around the age of 15, but not on the highest years of schooling and earnings.