Office of Environmental Management

Variation and tradeoffs within and among plant traits are increasingly being harnessed by empiricists and modelers to understand and predict ecosystem processes under changing environmental conditions. While fine roots play an important role in ecosystem functioning, fine-root traits are underrepresented in global trait databases. This has hindered efforts to analyze fine-root trait variation and link it with plant function and environmental conditions at a global scale. This Viewpoint addresses the need for a centralized fine-root trait database, and introduces the Fine-Root Ecology Database (FRED, http://roots.ornl.gov) which so far includes > 70 000 observations encompassing a broad range of root traits and also includes associated environmental data. FRED represents a critical step toward improving our understanding of below-ground plant ecology. For example, FRED facilitates the quantification of variation in fine-root traits across root orders, species, biomes, and environmental gradients while also providing a platform for assessments of covariation among root, leaf, and wood traits, the role of fine roots in ecosystem functioning, and the representation of fine roots in terrestrial biosphere models. Continued input of observations into FRED to fill gaps in trait coverage will improve our understanding of changes in fine-root traits across space and time.

The starchy swollen roots of cassava provide an essential food source for nearly a billion people, as well as possibilities for bioenergy, yet improvements to nutritional content and resistance to threatening diseases are currently impeded. A 454-based whole genome shotgun sequence has been assembled, which covers 69% of the predicted genome size and 96% of protein-coding gene space, with genome finishing underway. The predicted 30,666 genes and 3,485 alternate splice forms are supported by 1.4 M expressed sequence tags (ESTs). Maps based on simple sequence repeat (SSR)-, and EST-derived single nucleotide polymorphisms (SNPs) already exist. Thanks to the genome sequence, a high-density linkage map is currently being developed from a cross between two diverse cassava cultivars: one susceptible to cassava brown streak disease; the other resistant. An efficient genotyping-by-sequencing (GBS) approach is being developed to catalog SNPs both within the mapping population and among diverse African farmer-preferred varieties of cassava. These resources will accelerate marker-assisted breeding programs, allowing improvements in disease-resistance and nutrition, and will help us understand the genetic basis for disease resistance.

In this article we review the advances made in the 20th century in studying marriages. Progress moved from a self-report, personality-based approach to the study of interaction in the 1950s, following the advent of general systems theory. This shift led, beginning in the 1970s, to the rapid development of marital research using a multimethod approach. The development of more sophisticated observational measures in the 1970s followed theorizing about family process that was begun in the decade of the 1950s. New techniques for observation, particularly the study of affect and the merging of synchronized data streams using observational and self-report perceptual data, and the use of sequential and time-series analyses produced new understandings of process and power. Research in the decades of the 1980s and 1990s witnessed the realization of many secular changes in the American family, including the changing role of women, social science's discovery of violence and incest in the family, the beginning of the study of cultural variation in marriages, the expansion of the measurement of marital outcomes to include longevity, health, and physiology (including the immune system), and the study of comorbidities that accompany marital distress. A research agenda for the 21st century is then described.

Decision aids are evidence-based sources of health information that can help patients make informed treatment decisions. However, little is known about how decision aids affect health care use when they are implemented outside of randomized controlled clinical trials. We conducted an observational study to examine the associations between introducing decision aids for hip and knee osteoarthritis and rates of joint replacement surgery and costs in a large health system in Washington State. Consistent with prior randomized trials, our introduction of decision aids was associated with 26 percent fewer hip replacement surgeries, 38 percent fewer knee replacements, and 12-21 percent lower costs over six months. These findings support the concept that patient decision aids for some health conditions, for which treatment decisions are highly sensitive to both patients' and physicians' preferences, may reduce rates of elective surgery and lower costs.

Extensive data sets on water quality and seagrass distributions in Florida Bay have been assembled under complementary, but independent, monitoring programs. This paper presents the landscape-scale results from these monitoring programs and outlines a method for exploring the relationships between two such data sets. Seagrass species occurrence and abundance data were used to define eight benthic habitat classes from 677 sampling locations in Florida Bay. Water quality data from 28 monitoring stations spread across the Bay were used to construct a discriminant function model that assigned a probability of a given benthic habitat class occurring for a given combination of water quality variables. Mean salinity, salinity variability, the amount of light reaching the benthos, sediment depth, and mean nutrient concentrations were important predictor variables in the discriminant function model. Using a cross-validated classification scheme, this discriminant function identified the most likely benthic habitat type as the actual habitat type in most cases. The model predicted that the distribution of benthic habitat types in Florida Bay would likely change if water quality and water delivery were changed by human engineering of freshwater discharge from the Everglades. Specifically, an increase in the seasonal delivery of freshwater to Florida Bay should cause an expansion of seagrass beds dominated by Ruppia maritima and Halodule wrightii at the expense of the Thalassia testudinum-dominated community that now occurs in northeast Florida Bay. These statistical techniques should prove useful for predicting landscape-scale changes in community composition in diverse systems where communities are in quasi-equilibrium with environmental drivers. Corresponding Editor: W. M. Kemp.

An empirical model for predicting pressure drop across a cyclone, developed by Dirgo (1988), is presented.The model was developed through a statistical analysis of pressure drop data for 98 cyclone designs.The model is shown to perform better than the pressure drop models of Shepherd and Lapple (1940), Alexander (1949), First (1949), Stairmand (1949), and Barth (1956).This model is used with the efficiency model of --l o ~i a and Leith (1990) to develop an optimization curve which predicts the minimum pressure drop and the dimension ratios of the optimized cyclone for a given aerodynamic cut diameter, dS0.The effect of variation in cyclone height, cyclone diameter, and flow on the optimization is determined.The optimization results are used to develop a design procedure for optimized cyclones.

Communities around the world face increasingly severe fresh water supply shortages, largely due to expanding populations and associated food supply, economic development, and health issues. Intentional reuse of degraded waters (e.g., wastewater effluents, irrigation return flows, concentrated animal feeding operations [CAFO] effluents, stormwater, and graywater) as substitutes for fresh waters could be one solution to the challenge. We describe the various degraded water types and reuse options and limitations and restrictions to their use. Emphasis is given to reuse scenarios involving degraded water applications to soil. The potential for degraded water reuse is enormous, but significant barriers exist to widespread adoption. Barriers include research questions (some addressable by traditional soil science approaches, but others requiring novel techniques and advanced instrumentation), the lack of unifying national regulations, and public acceptance. Educational programs, based on hard science developed from long-term field studies, are imperative to convince the public and elected officials of the wisdom and safety of reusing degraded waters.

ABSTRACT The University of Texas Hydrate Pressure Coring Expedition (UT-GOM2-1) recovered cores at near in situ formation pressures from a gas hydrate reservoir composed of sandy silt and clayey silt beds in Green Canyon Block 955 in the deep-water Gulf of Mexico. The expedition results are synthesized and linked to other detailed analyses presented in this volume. Millimeter- to meter-scale beds of sandy silt and clayey silt are interbedded on the levee of a turbidite channel. The hydrate saturation (the volume fraction of the pore space occupied by hydrate) in the sandy silts ranges from 79% to 93%, and there is little to no hydrate in the clayey silt. Gas from the hydrates is composed of nearly pure methane (99.99%) with less than 400 ppm of ethane or heavier hydrocarbons. The δ13C values from the methane are depleted (−60‰ to −65‰ Vienna Peedee belemnite), and it is interpreted that the gases were largely generated by primary microbial methanogenesis but that low concentrations of propane or heavier hydrocarbons record at least trace thermogenic components. The in situ pore-water salinity is very close to that of seawater. This suggests that the excess salinity generated during hydrate formation diffused away because the hydrate formed slowly or because it formed long ago. Because the sandy silt deposits have high hydrate concentration and high intrinsic permeability, they may represent a class of reservoir that can be economically developed. Results from this expedition will inform a new generation of reservoir simulation models that will illuminate how these reservoirs might be best produced.

The traveler participated in a conference on environmental technology in Paris, sponsored by the US Embassy-Paris, US Environmental Protection Agency (EPA), the French Environmental Ministry, and others. The traveler sat on a panel for environmental aspects of energy technology and made a presentation on the potential contributions of Oak Ridge National Laboratory (ORNL) to a planned French-American Environmental Technologies Institute in Chattanooga, Tennessee, and Evry, France. This institute would provide opportunities for international cooperation on environmental issues and technology transfer related to environmental protection, monitoring, and restoration at US Department of Energy (DOE) facilities. The traveler also attended the Fourth International Conference on Environmental Contamination in Barcelona. Conference topics included environmental chemistry, land disposal of wastes, treatment of toxic wastes, micropollutants, trace organics, artificial radionuclides in the environment, and the use biomonitoring and biosystems for environmental assessment. The traveler presented a paper on The Fate of Radionuclides in Sewage Sludge Applied to Land.'' Those findings corresponded well with results from studies addressing the fate of fallout radionuclides from the Chernobyl nuclear accident. There was an exchange of new information on a number of topics of interest to DOE waste management and environmental restoration needs.

An epidemiologic investigation was done after 3 patients contracted Ochrobactrum anthropi meningitis at one hospital in October 1994. Neurosurgical patients with pericardial tissue implants were at greater risk of infection than other neurosurgical patients (3/14 vs. 0/566; P<.001). Cultures of implants removed from 2 case-patients, an implant at implantation, a nonimplanted pericardial tissue, and an unwrapped but unopened bottle of Hank's balanced salt solution (HBSS) grew O. anthropi. Patient and tissue isolates had identical genotypes; the isolate from the HBSS bottle had a unique genotype. Culture samples from an unopened HBSS bottle and from pericardial tissue grew Pseudomonas stutzeri of the same genotype; however, no P. stutzeri infections were detected. The investigation documented intrinsic P. stutzeri contamination of HBSS. O. anthropi contamination of tissues occurred during processing, possibly due to extrinsic contamination of HBSS. Active surveillance is needed to detect infection in patients receiving transplanted tissues, and rigorous infection control practice are necessary during tissue harvesting and processing to ensure sterility.

As company supply chains grow larger, more dispersed, and more complex, companies and their stakeholders are increasingly interested in knowing more about these supply chains and requesting greater transparency regarding them. This article examines several aspects that can assist companies in building greater transparency in supply chains, including supply chain mapping, traceability, third-party certification, and goal setting, as well as reporting on their progress toward achieving these efforts. In addition, this article provides summaries of how several companies are building greater transparency in their supply chains, efforts that also help companies better manage the environmental impacts from their supply chains.

Organic-inorganic perovskite solar cells (PSCs) have recently emerged as a potential candidate for large-scale and low-cost photovoltaic devices. However, the technology is still susceptible to degradation issues and toxicity concerns due to the presence of lead (Pb). Therefore, investigation on ideal methods to deal with PSC wastes once the device attains its end-of-life is crucial and to recycle the components within the cell is the most cost effective and energy effective method by far. This paper reported on a layer-by-layer extraction approach to recycle the fluorine-doped tin oxide (FTO) coated glass substrate which is the most expensive component in the device architecture of mesoporous planar PSC. By adapting the sequential removal of each layer, chemical properties of individual components, including spiro-OMeTAD and gold can be preserved, enabling the material to be easily reused. It also ensured that the toxic Pb component could be isolated without contaminating other materials. The removal of all individual layers allows the retrieval of FTO conductive glass which can be used in various applications that are not only restricted to photovoltaics. Comparison of electrical, morphological and physical properties of recycled FTO glasses to commercial ones revealed minimal variations. This confirmed that the recycling approach was useful in retrieving the substrate without affecting its physicochemical properties.

In this brief commentary we provide some parallel points to complement Steve Keen’s paper in the recent Globalization’s special forum on ‘Economics and Climate Emergency’. Keen’s critique of climate and economy Integrated Assessment Models (IAMs) is wide-ranging, but there is still scope to bring to the fore the general issues that help to make sense of the critique. Accordingly, we set out six key inadequacies of IAMs and argue towards the need for a different approach that is more realistic regarding the limits to growth.

The rate of generation of molecular sequence data is forcing the use of computers as a central tool in molecular biology. Current use of computers is limited largely to data management and sequence comparisons, but rapid growth in the volume of data is generating pressure for the development of high-speed analytical methods for deciphering the codes connecting nucleotide sequence with protein structure and function.

Photovoltaic thermal (PVT) systems are increasingly becoming an essential part of the solar application systems integrating the photovoltaic (PV) and solar thermal collectors into a single unit to produce heat and electrical energy from the intermittent solar irradiation.

Lagos has been identified as one of the 50 cities most vulnerable to extreme sea levels. The state also ranked 30th among 136 port cities in terms of population exposure to flooding under a past climate scenario (2005) and 15th under a future climate scenario (2070s). The state faces significant environmental strains as a result of its geo-economic status. Some of the problems are wetland loss, pollution, population pressure, restricted access to drinkable water, and flooding. All these factors have contributed to the instability of Lagos ecosystems, but the impact of flooding is particularly significant because Lagos is surrounded by aquatic ecosystems and its low elevation and topography increase its susceptibility to flooding. The method adopted in this review involved the use of PICO (Population, Intervention, Control, and Outcomes) criteria to synthesize the research questions and objectives. Thereafter, PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines was employed for the study selection criteria, search strategies and data extraction methods. A broad search strategy involving databases (Google scholar, Science Direct), pertinent keywords and search filters was used to identify relevant articles and minimize selection bias. After the search, PICO criteria was again used to select the studies to be considered in the review. The selected text were downloaded and the essential scientific information were extracted and analyzed in the study. The impacts of flooding are numerous. Flooding is a key factors that has prevented Africa’s growing urban population from escaping poverty, and it also impedes the achievement of some SDGs. This is because many African cities lack the resources and infrastructures needed to withstand extreme weather conditions. Surviving in flood-prone cities like Lagos is a daunting task because flood affects livelihood, human health, and can even cause death. Specifically, the 2020 flood incidence in Nigeria affected about 40% of the local government areas, and 97% of the states, displacing over 120,000 persons and killing 68 persons aside from properties and farmlands destroyed. Women and children are the most vulnerable. The adoption of advanced flood risk management strategies could help in flood containment and management in the state.

The green fluorescent protein gene (gfp) was introduced into a p-nitrophenol-metabolizing strain of Moraxella sp. by chromosomal integration. The gfp-marked transformants, designated Moraxella sp. strains G21 and G25, exhibited green fluorescence under UV light. Molecular characterization by PCR and Southern hybridization showed the presence of gfp in both transformants. Both transformants and the parent strain degraded 720 microM of p-nitrophenol with nitrite release within 4 h after inoculation in minimal medium supplemented with yeast extract. Transformants degraded up to 1440 microM p-nitrophenol and mineralized about 60% of 720 microM p-nitrophenol, both in broth and in soil, to the same extent as the parent strain. Insertion of gfp did not adversely affect the expression of p-nitrophenol-degrading genes in the transformants. Survival studies indicated that individual green fluorescent colonies of transformants can be detected up to 2 weeks after inoculation in soil. These marked strains could be of value in studies on microbial survival in the environment.

In Germany, nine permeable reactive barriers (PRBs) for passive in situ remediation of contaminated groundwater have been erected over the last 3 to 4 years (in Bernau, built 2001; Bitterfeld, 1999; Denkendorf, 2000; Edenkoben, 1998 and 2001; Karlsruhe, 2000; Oberursel, 2002; Reichenbach, 2000; Rheine, 1998; and Tübingen, 1998), all revealing interesting design and engineering features. At the Edenkoben site, one can find what is probably one of today’s largest funnel-and-gate (F&G) systems (approximately 450 m long, equipped with six gates). This paper provides an introduction to German PRB projects, focusing on design and engineering features as well as on some major first outcomes regarding contaminant destruction efficiency and long-term performance, where available. It shows the prevalence of F&G and related systems, which are, predominantly equipped with specially positioned or designed funnels and/or gates (for example, relatively flat gates installed close below ground level, or reactors receiving passively or even actively diverted or lifted groundwater). Different zero valent iron types or activated carbon are the reactive materials exclusively applied in German PRBs to treat chlorinated volatile organic carbons and polycyclic aromatic hydrocarbons (PAHs), though a biological treatment zone to degrade PAHs is planned to be set up at Offenbach, and different alternative innovative materials are currently being tested in a semitechnical scope at Bitterfeld and elsewhere.

Milling activities at a former uranium mill site near Riverton, Wyoming, USA, contaminated the shallow groundwater beneath and downgradient of the site. Although the mill operated for <6 years (1958–1963), its impact remains an environmental liability. Groundwater modeling predicted that natural flushing would achieve compliance with applicable groundwater protection standards by the year 2098. A decade of groundwater monitoring indicated that contaminant concentrations were declining steadily, which confirmed the conceptual site model (CSM). However, local flooding in 2010 mobilized contaminants that migrated downgradient from the Riverton site and resulted in a dramatic increase in groundwater contaminant concentrations. This observation indicated that the original CSM was inadequate to explain site conditions and needed to be refined. In response to the new observations after the flood, a collaborative investigation to better understand site conditions and processes commenced. This investigation included installing 103 boreholes to collect soil and groundwater samples, sampling and analysis of evaporite minerals along the bank of the Little Wind River, an analysis of evapotranspiration in the shallow aquifer, and sampling naturally organic-rich sediments near groundwater discharge areas. The enhanced characterization revealed that the existing CSM did not account for high uranium concentrations in groundwater remaining on the former mill site and groundwater plume stagnation near the Little Wind River. Observations from the flood and subsequent investigations indicate that additional characterization is still needed to continue refining the CSM and determine the viability of the natural flushing compliance strategy. Additional sampling, analysis, and testing of soil and groundwater are necessary to investigate secondary contaminant sources, mobilization of contaminants during floods, geochemical processes, contaminant plume stagnation, distribution of evaporite minerals and organic-rich sediments, and mechanisms and rates of contaminant transfer from soil to groundwater. Future data collection will be used to continually revise the CSM and evaluate the compliance strategy at the site.

This study investigated the stability and the in vitro digestion of curcumin nanoemulsions stabilized by three protein hydrolysates: peanut protein isolate (PPI), soybean protein isolate (SPI) and whey protein isolate (WPI). After enzymatic hydrolysis, the protein structure became more disordered, and increased antioxidant capacity was also observed for protein hydrolysates. The protein hydrolysates generated curcumin nanoemulsions with considerable stability over 28 days of storage. Moreover, protein hydrolysates more effectively improved the lipolysis rate and bioaccessibility of curcumin nanoemulsions than native proteins, and PPI hydrolysates exhibited the highest lipolysis rate (110.43%) and the highest bioaccessibility (53.24%). This study indicated that protein hydrolysates could be used as emulsifiers for preparing nanoemulsion delivery systems with high stability and bioaccessibility.