University of the Philippines Visayas

Fungi are an understudied, biotechnologically valuable group of organisms. Due to the immense range of habitats that fungi inhabit, and the consequent need to compete against a diverse array of other fungi, bacteria, and animals, fungi have developed numerous survival mechanisms. The unique attributes of fungi thus herald great promise for their application in biotechnology and industry. Moreover, fungi can be grown with relative ease, making production at scale viable. The search for fungal biodiversity, and the construction of a living fungi collection, both have incredible economic potential in locating organisms with novel industrial uses that will lead to novel products. This manuscript reviews fifty ways in which fungi can potentially be utilized as biotechnology. We provide notes and examples for each potential exploitation and give examples from our own work and the work of other notable researchers. We also provide a flow chart that can be used to convince funding bodies of the importance of fungi for biotechnological research and as potential products. Fungi have provided the world with penicillin, lovastatin, and other globally significant medicines, and they remain an untapped resource with enormous industrial potential.

The expansion of clothing and textile industry and the fast fashion trend among consumers have caused a rapid global increase in textile waste in the municipal solid waste (MSW) stream. Worldwide, 75% of textile waste is landfilled, while 25% is recycled or reused. Landfilling of textile waste is a prevalent option that is deemed unsustainable. Promoting an enhanced diversion of textile waste from landfills demands optimized reuse and recycling technologies. Reuse is the more preferred option compared with recycling. Various textile reuse and recycling technologies are available and progressively innovated to favor blended fabrics. This paper aims to establish reuse and recycling technologies (anaerobic digestion, fermentation, composting, fiber regeneration, and thermal recovery) to manage textile waste. Improved collection systems, automation of sorting, and discovering new technologies for textile recycling remains a challenge. Applying extended producer responsibility (EPR) policy and a circular economy system implies a holistic consensus among major stakeholders.

The purpose of this paper is to extend the understanding of the drivers of social media in higher education institutions (HEIs) in an emerging economy. This research adopts the Technology Acceptance Model but included subjective norm, perceived playfulness, Internet reliability and speed as additional constructs. With these inclusions, the model is appropriate and relevant in explaining users’ adoption and usage behavior of social media. Data from 500 students from public and private HEIs in the Philippines were collected and analyzed. We used a combination of statistical analyses such as the Principal Component Analysis (PCA) and Structural Equation Modeling (SEM) in analysing the complex relationships between determinants of these technologies. The research demonstrated that perceived usefulness, perceived ease of use, subjective norm, and perceived playfulness (happiness) are robust predictors of usage behavior of students. However, Internet reliability and speed were only significant in (some) public HEIs. This evidence may be explained by the fact that information and communications technology (ICT) infrastructure in public HEIs is not a priority or underinvested in developing countries. On the other hand, the analysis between public and private HEIs undertaken here extends our understanding towards the different behaviors of users. The findings, though preliminary, suggest that private HEIs should initiate or continue the use of social media in classrooms, because intention to use translate to actual use of these tools. Public institutions, however, should improve Internet reliability and speed and should reassess their use of social media in order to fully take advantage of the benefits of ICT.

Effectively reducing climate change requires marked, global behavior change. However, it is unclear which strategies are most likely to motivate people to change their climate beliefs and behaviors. Here, we tested 11 expert-crowdsourced interventions on four climate mitigation outcomes: beliefs, policy support, information sharing intention, and an effortful tree-planting behavioral task. Across 59,440 participants from 63 countries, the interventions' effectiveness was small, largely limited to nonclimate skeptics, and differed across outcomes: Beliefs were strengthened mostly by decreasing psychological distance (by 2.3%), policy support by writing a letter to a future-generation member (2.6%), information sharing by negative emotion induction (12.1%), and no intervention increased the more effortful behavior-several interventions even reduced tree planting. Last, the effects of each intervention differed depending on people's initial climate beliefs. These findings suggest that the impact of behavioral climate interventions varies across audiences and target behaviors.

The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide pandemic causing substantial morbidity, mortality, and economic devastation. In response, many laboratories have redirected attention to SARS-CoV-2, meaning there is an urgent need for tools that can be used in laboratories unaccustomed to working with coronaviruses. Here we report a range of tools for SARS-CoV-2 research. First, we describe a facile single plasmid SARS-CoV-2 reverse genetics system that is simple to genetically manipulate and can be used to rescue infectious virus through transient transfection (without in vitro transcription or additional expression plasmids). The rescue system is accompanied by our panel of SARS-CoV-2 antibodies (against nearly every viral protein), SARS-CoV-2 clinical isolates, and SARS-CoV-2 permissive cell lines, which are all openly available to the scientific community. Using these tools, we demonstrate here that the controversial ORF10 protein is expressed in infected cells. Furthermore, we show that the promising repurposed antiviral activity of apilimod is dependent on TMPRSS2 expression. Altogether, our SARS-CoV-2 toolkit, which can be directly accessed via our website at https://mrcppu-covid.bio/, constitutes a resource with considerable potential to advance COVID-19 vaccine design, drug testing, and discovery science.

Abstract Seaweeds have been used as a food for centuries in Asia and are increasingly exploited as a source for dietary supplements, animal feed, chemicals, and biofuels. In recent years, there has been an increase in the prevalence of diseases and pests in these aquaculture crops, with a subsequent reduction in their quantity and commercial value. In this article, we review diseases that have been reported in the scientific literature for species of red and brown seaweeds. We have focused on the major seaweed crops grown in Asia, where much of this production is centered. We also provide information on disease management and biosecurity and some observations on future directions.

. All these taxa are supported by morphological and multigene phylogenetic analyses. This article allows the researchers to publish fungal collections which are important for future studies. An updated, accurate and timely report of fungus-host and fungus-geography is important. We also provide an updated list of fungal taxa published in the previous fungal diversity notes. In this list, erroneous taxa and synonyms are marked and corrected accordingly.

Disaster response work is associated with various psychological outcomes. In post-disaster conditions, social support is generally observed to impact mental health, particularly for survivors. This review was conducted to survey the extent of social support effectiveness on disaster responder groups. Published quantitative social support studies on police, emergency medical responders, rescue and recovery workers, firefighters, and military responders were searched in various academic databases using keyword searches, a reference list search, and a citation search that resulted in 24 studies with 90 effect sizes being included in the final data base. Articles were coded and effect sizes were averaged using the Hedges–Vevea Random Effects model. Nineteen categories of psychological outcomes (for example, anxiety, depression, posttraumatic stress symptoms, and psychological distress) and eight classifications of support were coded. Social support was found to be associated with anxiety, burnout, depression, job control, job satisfaction, psychological distress, turnover intentions, and work engagement, with mean effect sizes from − 0.36 to 0.57. Most studies measured perceived social support and negative outcomes. Social support correlated with outcomes in police responders and rescue and recovery workers. This review discusses the breadth of effect of social support, as well as other elements, such as temporal factors, that may affect the effectiveness of social support in disaster responders.

Salinity is a major abiotic stress affecting plant growth and productivity. Considerable genetic variation is present in rice in response to salt stress, with higher sensitivity during early seedling and reproductive stage. In this study, physiological changes in leaves and developing panicles of rice genotypes (IR686, Sadri, Rc222, CSR28, IR670 and Pokkali) contrasting in salt tolerance at the reproductive stage were evaluated in greenhouse experiment under salt stress. The results showed that IR670 and the tolerant-check Pokkali maintained lower Na+/K+ ratio, less reduction in chlorophyll concentration, lower malondialdehyde (MDA) production, higher concentrations of reduced ascorbate (reduced AsA), higher proline accumulation and lower percentage reduction in pollen viability than the salt-sensitive genotypes under salt stress. The higher concentration of reduced AsA suggests an efficient ROS-scavenging system. Physiological measurements and pollen viability analysis revealed that Sadri (moderately tolerant at the seedling stage) is sensitive to salt stress at the flowering stage. The findings will be useful in breeding salt tolerant varieties at both seedling and reproductive stages by selecting appropriate genotypes and phenotypes. Keywords: Na+/K+ ratio, Proline, Reproductive stage, Rice, Salinity stress, Reduced ascorbate, Pollen viability

Charcoal briquettes are inexpensive solid fuels made from carbonized biomass. The potential of converting water hyacinth (Eichhornia crassipes) charcoal into briquettes with molasses as binder was investigated in this study. Dried water hyacinth was carbonized at a temperature between 350°C to 500°C in a fabricated fine biomass carbonizer. A solution containing 80% by weight molasses was used in the production of briquettes having different charcoal/molasses ratios of 40:60, 30:70, and 20:80. Each briquette was characterized in terms of bulk density, calorific value, compressive strength, proximate analysis and micro-structure by Scanning Electron Microscopy. Charcoal briquettes were tested for their flammable characteristics through their burning rates and ignition time. Altering the molasses to charcoal ratio affected the quality and characteristics of the briquettes. Volatile combustible matter and fixed carbon increased with increasing amount of binder while ash content decreased. The 30:70 charcoal/molasses ratio produced the highest calorific value (16.6 MJ/kg) and compressive strength (19.1 kg/cm2). The results have shown the potential of converting water hyacinth into an alternative fuel source.

The general purpose of this study is to determine the issues and challenges of special education (SPED) teachers in teaching children with learning disabilities in the City Division of Ilagan Isabela, Philippines. The 15 SPED teachers were served as the respondents of this study using purposive sampling technique. Qualitative Research Method (QRM) was utilized to determine the issues and challenges of the teachers. Thematic analysis was employed to interpret the data. The analyses on the sharing of the key informants regarding their collective description of their challenges as SPED teacher led to the emergence of five distinct themes that include, choosing appropriate strategy and motivation; identifying individual needs; challenging but fulfilling; acceptance and patience; and respect one’s right. Most teachers teaching children with learning disabilities did not receive any special needs education training from the school, they feel that they are not qualified to teach the children with learning disability. Moreover, teachers assigned in SPED classes lack of strategies in dealing with learners with disabilities. This study revealed that the classrooms for children with learning disabilities in Division of Ilagan at large have poor learning environment to support the SPED such as lack of budget, curriculum guide, Instructional Materials (IMs) and even school facilities. It could be concluded that placement of learners with special needs in an inclusive classroom with ordinary learners is not enough with no proper support. Learners with disability did not receive all the necessary support and services for accessing the curriculum facilities; and stakeholders’ supports is very minimal to support the needs of the students enrolled in SPED classes. On the other hand, issues and problems were solved technically to sustain the positive working environment among school heads, teachers and stakeholders. The Department of Education Training and Development in collaboration with regional in service officers should organize continuous professional development opportunities on inclusion strategies of learners with SPED needs. The implementers of the SPED programs shall strictly adhere to the policies, and the strong support of the stakeholders shall be encouraged by formulating active organization spearheaded by the school head.

Mangroves provide valuable ecological and socio-economic services. The importance of mangroves is particularly evident in Southeast (SE) Asia where the most extensive and diverse forests are found. To recover degraded mangroves, several SE Asian countries have implemented restoration programs. However, to date, there has been no systematic and quantitative synthesis on mangrove restoration studies in the region. Here, we provide a bibliometric-based analysis of mangrove restoration to provide understanding on trends and future directions needed to meet biodiversity and restoration targets in the region. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol, we analyzed 335 articles (249 articles with ecological attributes; 86 articles with social attributes) published until February 2022 from Scopus and Web of Science databases. Mangrove restoration studies with ecological and social attributes started around the early 1990s mostly from Indonesia, Thailand, Malaysia, Vietnam, and the Philippines. Majority of SE Asian countries have stronger collaboration to western countries rather than within the region. Reasons for restoration vary per country, but mostly were intended to rehabilitate damaged mangroves. Direct planting was the most common restoration method used while hydrological rehabilitation was less practiced. Research on ecological attributes were dominated by biodiversity-related studies focused on flora and fauna, and less on other ecosystem services (e.g., coastal protection, fisheries production, etc.). Studies with social attributes only accounted for <30% of the publications, mostly on topics related to ecological economics. Although mangrove restoration studies are apparent, some thematic restoration foci are needed. We propose priority research topics to help achieve the biodiversity and restoration targets by 2030.

Abstract. Multi-hazard risk assessment of building portfolios is of primary importance in natural-hazard-prone regions, particularly for the prioritisation of disaster risk reduction and resilience-enhancing strategies. In this context, cultural heritage assets require special consideration because of their high vulnerability to natural hazards – due to ageing and types of construction – and their strong links with communities from both an economic and a historical–sociocultural perspective. This paper introduces a multi-hazard risk prioritisation framework specifically developed for cultural heritage assets. The proposed framework relies on a multilevel rapid-visual-survey (RVS) form for the multi-hazard exposure data collection and risk prioritisation of case-study assets. Because of the multilevel architecture of the proposed RVS form, based on three levels of refinement and information, an increasing degree of accuracy can be achieved in the estimation of structural vulnerability and, ultimately, structural risk of the considered assets. At the lowest level of refinement, the collected data are used for the computation of seismic-risk and wind-risk prioritisation indices, specifically calibrated in this study for cultural heritage assets with various structural and non-structural features. The resulting indices are then combined into a unique multi-hazard risk prioritisation index in which the intangible value of cultural heritage assets is also considered. This is achieved by defining a score expressing the cultural significance of the asset. The analytic hierarchy process is extensively used throughout the study to reduce the subjectivity involved in the framework, thus obtaining a simplified yet robust approach which can be adapted to different building typologies. The proposed framework is applied to 25 heritage buildings in Iloilo City, Philippines, for which innovative, non-invasive techniques and tools for improved surveying have also been tested. Thermal and omnidirectional cameras have helped in the collection of structural data, together with drones for the inspection of roofs. Results of the study are presented and critically discussed, highlighting advantages and drawbacks of the use of new technologies in this field.

The Coral Triangle Marine Protected Area System aspires to become a region-wide, comprehensive, ecologically representative and well-managed system of marine protected areas (MPAs) and MPA networks. The development of this system will proceed primarily through the implementation of ecological, social, and governance MPA networks at the sub-national scale. We describe six case studies that exemplify different approaches taken to develop MPA networks in the Coral Triangle region at different scales: Nusa Penida in Indonesia; Tun Mustapha Park in Malaysia; Kimbe Bay in Papua New Guinea; Verde Island Passage in the Philippines; The Lauru Ridges to Reefs Protected Area Network in Choiseul, Solomon Islands; and Nino Konis Santana Park in Timor Leste. Through synthesis of these case studies, we identify five common themes that contributed to successful outcomes: (1) the need for multi-stakeholder and cross-level management institutions; (2) the value of integrating cutting-edge science with local knowledge and community-based management; (3) the importance of building local capacity; (4) using multiple-use zoning to balance competing objectives; and (5) participation in learning and governance networks. These lessons will be invaluable in guiding future efforts to expand the Coral Triangle Marine Protected Area System, and provide important insights for MPA practitioners elsewhere.

Farming households in the Philippines are most vulnerable to climate change and variability due to their climate/weather-sensitive livelihood and lack of resources to finance adaptation measures. In order to formulate appropriate programs and policies addressing this vulnerability, it is essential to understand their adaptive capacity.This study analyzed the adaptive capacity to climate change and vulnerability of 520 farming households in Dumangas, a town in central Philippines confronting climate/weather-induced risks. The objectives were: to determine the levels of adaptive capacity of farming households to climate change, analyze the factors that cause the differences in adaptive capacity and find out whether adaptive capacity translates to adaptation. The level of adaptive capacity of each farming household was determined using a composite index based on previous studies. The index included five indicators namely human resources, physical resources, financial resources, information and diversity. Results showed that variations in adaptive capacity were caused by differences in information resources, physical and financial resources. Farming households that scored low in these three indicators had lower adaptive capacity. It was also found out that despite their level of adaptive capacity, households employed measures to adapt to climate change and variability. Households with higher adaptive capacity however employed more adaptation strategies.

A 50-day growth trial was conducted to evaluate the efficacy of Chlorella vulgaris (Beijerinck) as an ingredient in the diets of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae (PL30). Immune response (total haemocyte count and prophenoloxidase activity) was also assessed by subjecting postlarvae to a challenge test against Aeromonas hydrophila (Chester) for 14 days. Iso-nitrogenous and iso-lipidic test diets were prepared using a fishmeal-based-positive control diet (D0) and four basal diets with inclusion levels of 2% (D2), 4% (D4), 6% (D6) and 8% (D8) C. vulgaris. Postlarvae of M. rosenbergii were randomly stocked (mean initial body weight of 0.19 ± 0.02 g) in 30-L tanks in three replicates per dietary treatment for evaluation of growth performance. Another set of postlarvae (mean initial body weight of 1.25 ± 0.02 g) was randomly distributed in 95-L tanks in three replicates per dietary treatment for the assessment of immune response. Results showed that specific growth rate was significantly higher (P < 0.05) in postlarvae fed D4 and D6. Variations in values for carcass protein, lipid, moisture and ash were also evident. Postlarvae fed diets with Chlorella showed increased prophenol oxidase activity and total haemocyte counts. Moreover, survival rate after challenge with A. hydrophila was significantly increased (P < 0.05). Inclusion of C. vulgaris in diets enhanced immune response and resistance of M. rosenbergii postlarvae against A. hydrophila infection.

Societal Impact Statement Seaweed cultivation is the fastest‐growing aquaculture sector, with a demonstrable potential to drive development in some of the poorest coastal populations worldwide. However, sustainable exploitation, fair access and equitable benefits from marine genetic resources, such as seaweeds have yet to be fully realised. Patchy fundamental knowledge on the genetic diversity and metabolic potential of algae limits their exploitation; scant practical skills and low investment in breeding restricts germplasm availability and the Nagoya protocol has only partially remediated insufficient governance. Further developments and the addressing of knowledge gaps in relation to biosecurity, breeders’ rights and conservation of genetic resources are needed for progress. Summary We review how seaweed genetic resources are currently used in aquaculture, in relation to the diversification and rapidly increasing use of marine resources. Using a revealing case‐study, we summarise the potential for positive societal change, underpinned by the cultivation of eucheumatoid carrageenophytes (species of the red algal genera Eucheuma and Kappaphycus ), an activity which has been successfully initiated in many tropical countries to support their economic development. We also review the challenges currently faced by this industry and identify potential threats to the seaweed cultivation sector. Accordingly, we suggest new directions to support the continued development of an economically resilient and environmentally sustainable industry based on the utilisation of genetic resources.

There are three groups of scientists dominating the search for the origin of life: the organic chemists (the Soup), the molecular biologists (RNA world), and the inorganic chemists (metabolism and transient-state metal ions), all of which have experimental adjuncts. It is time for Clays and the Origin of Life to have its experimental adjunct. The clay data coming from Mars and carbonaceous chondrites have necessitated a review of the role that clays played in the origin of life on Earth. The data from Mars have suggested that Fe-clays such as nontronite, ferrous saponites, and several other clays were formed on early Mars when it had sufficient water. This raised the question of the possible role that these clays may have played in the origin of life on Mars. This has put clays front and center in the studies on the origin of life not only on Mars but also here on Earth. One of the major questions is: What was the catalytic role of Fe-clays in the origin and development of metabolism here on Earth? First, there is the recent finding of a chiral amino acid (isovaline) that formed on the surface of a clay mineral on several carbonaceous chondrites. This points to the formation of amino acids on the surface of clay minerals on carbonaceous chondrites from simpler molecules, e.g., CO2, NH3, and HCN. Additionally, there is the catalytic role of small organic molecules, such as dicarboxylic acids and amino acids found on carbonaceous chondrites, in the formation of Fe-clays themselves. Amino acids and nucleotides adsorb on clay surfaces on Earth and subsequently polymerize. All of these observations and more must be subjected to strict experimental analysis. This review provides an overview of what has happened and is now happening in the experimental clay world related to the origin of life. The emphasis is on smectite-group clay minerals, such as montmorillonite and nontronite.

BACKGROUND: Mud crabs, Scylla spp., are commercially important large-size marine crustaceans in the Indo-West Pacific region. As females have the higher growth rate and economic value, the production of all female stocks is extremely essential in aquaculture. However, the sex determination mechanism is still unclear. Development of sex-specific genetic markers based on next-generation sequencing proved to be an effective tool for discovering sex determination system in various animals. RESULTS: Restriction-site associated DNA sequencing (RAD-seq) was employed to isolate sex-specific SNP markers for S. paramamosain. A total of 335.6 million raw reads were obtained from 20 individuals, of which 204.7 million were from 10 females and 130.9 million from 10 males. After sequence assembly and female-male comparison, 20 SNP markers were identified to be sex-specific. Furthermore, ten SNPs in a short sequence (285 bp) were confirmed heterozygous in females and homozygous in males in a large population by PCR amplification and sequencing. Subsequently, a female-specific primer was successfully designed according to the female-specific nucleotide which could amplify an expected band from females but not from males. Thus, a rapid and effective method for molecular sexing in S. paramamosain was developed, meanwhile, this method could successfully identify the sex of S. tranquebarica and S. serrata. Finally, nine and four female-specific SNP markers were detected in S. tranquebarica and S. serrata, respectively. CONCLUSIONS: Sex-specific SNP markers were firstly identified in crab species and showed female heterogamety and male homogamety, which provided strong genetic evidence for a WZ/ZZ sex determination system in mud crabs S. paramamosain, S. tranquebarica and S. serrata. These findings will lay a solid foundation for the study of sex determination mechanism, sex chromosome evolution, and the development of mono-sex population in crustaceans.

Two separate experiments were conducted to determine the dietary requirements of juvenile Asian sea bass Lates calcarifer Bloch for lysine and arginine. Fish (average initial weight: lysine experiment, 13.12 ± 0.12 g; arginine experiment, 2.56 ± 0.13 g) were given amino acid test diets for 12 weeks containing fish meal, zein, squid meal, and crystalline amino acids. Each set of isonitrogenous and isocaloric test diets contained graded levels of L-lysine or L-arginine. The feeding rate in the lysine experiment was at 4–2.5% of the body weight day−1, while in the arginine experiment it was at 10–4% of the body weight day−1. The fish (20 per tank, lysine experiment; 15 per tank, arginine experiment) were reared in 500-L fibreglass tanks with continuous flowthrough sea water at 27 °C and salinity of 31 ppt in the lysine experiment and at 29 °C and salinity of 29 ppt in the arginine experiment. The experiments were in a completely randomized design with two replicates per treatment. Survival was high in fish given adequate lysine or arginine. Mean percentage weight gains were significantly different in fish fed varying levels of lysine or arginine. Fish fed high levels of L-arginine suffered high mortalities. No significant differences were obtained in the feed efficiency ratios (FER, g gain g−1 feed) of fish fed graded lysine, although the values tended to increase as the dietary lysine level was increased up to the requirement level. In contrast, in the arginine experiment, significant differences in FER of fish among treatments were obtained; the highest FER was observed in fish fed the diet containing an optimum arginine level. On the basis of the growth response, survival, and FER, the lysine and arginine requirements of juvenile Asian sea bass were estimated to be 20.6 g kg−1 dry diet (4.5% protein) and 18.2 g kg−1 dry diet (3.8% protein), respectively. These data will be useful in the further refinement of practical diet formulations for the Asian sea bass.