A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes

Janke, Carsten; M. Magiera, Maria; Rathfelder, Nicole; Taxis, Christof; Reber, Simone; Maekawa, Hiromi; Moreno‐Borchart, Alexandra; Doenges, Georg; Schwob, Etienne; Schiebel, Elmar; Knop, Michael

doi:10.1002/yea.1142

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A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes

Shared by NobleBlocks on Aug 1, 2004 • 12:00 AM UTC

Authors:

Carsten Janke

Maria M. Magiera

Nicole Rathfelder

Abstract

Abstract Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo in the yeast Saccharomyces cerevisiae . This strategy directs the amplified tags to the desired chromosomal loci due to flanking homologous sequences provided by...

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