PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.

RD, Saunders; DM, Glover; M, Ashburner; I, Siden-Kiamos; C, Louis; M, Monastirioti; C, Savakis; F, Kafatos

doi:10.1093/nar/17.22.9027

Public

PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.

Shared by NobleBlocks on Nov 1, 1989 • 12:00 AM UTC

Authors:

Saunders RD

Glover DM

Ashburner M

Abstract

A novel alternative to microcloning for the production of region specific chromosomal DNA is described. In this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with Sau3A. Oligonucleotide adaptors are ligated to these fragments to provide convenient pr...

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