Detection of
                    <i>Mycobacterium tuberculosis</i>
                    in Sputum Samples Using a Polymerase Chain Reaction

D. Eisenach, Kathleen; D. Sifford, Mark; Donald Cave, M.; H. Bates, Joseph; T. Crawford, Jack

doi:10.1164/ajrccm/144.5.1160

Public

Detection of Mycobacterium tuberculosis in Sputum Samples Using a Polymerase Chain Reaction

Shared by NobleBlocks on Nov 1, 1991 • 12:00 AM UTC

Authors:

Kathleen D. Eisenach

Mark D. Sifford

M. Donald Cave

Abstract

Abstract A polymerase chain reaction (PCR) assay for the rapid detection of Mycobacterium tuberculosis in sputum samples is described. The target DNA is a 123-base pair (bp) segment of IS6110, which is repeated in the M. tuberculosis chromosome and is specific for the M. tuberculosis complex. Method...

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