A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source. A high sensitivity cooled charge-coupled device (CCD) camera with excitation and emission filters is utilized to obtain the excitation and fluorescence images. The laser beam performs fast raster scanning using a dual-axis galvanometric scanner. The accuracy of the laser spot position at the source window is within 200 m. Based on the phantom experimental results, the spatial resolution is less than 1.7 mm, and the relative quantitation error is about 10%. In vivo imaging of a tumor-bearing nude mouse tagged with near-infrared dye demonstrates the feasibility of the system.