This article appears in the Oxford Handbook of Sound and Image in Digital Media edited by Carol Vernallis, Amy Herzog, and John Richardson. This chapter attempts to think through the implications of eco-criticism in new media, especially for the theory of subjectivity. Because eco-criticism expands the concept of agency to nonhuman actors, it challenges older, exclusively humanist conceptions of subjectivity. Eco-critical perspectives on cinematic ontology, epistemology, and object formation (are we looking at organisms, ecologies, or a planetary system?) must also extend, in the age of informatics, to understanding what such extension implies for the human subject, both as matter of representation and as the site of interpretation and understanding. Source Code (Duncan Jones, 2011) offers itself as a laboratory for conducting research into the becoming-environment of information and what that implies for human subjectivity.