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Substrate translocation via Mep2<sup>H194E</sup> induces a pH decrease.

Published in Figshare • Feb 18, 2020
NobleIDNI2P684W840R365S778
Authors:
Ana Sofia Brito (8463537)
,
Benjamin Neuhäuser (8463540)
,
René Wintjens (237881)

Abstract

<p>Haploid triple-<i>mepΔ</i> cells (31019b) were transformed with pMep2-pHluorin (A), pMep2<sup>D186N</sup>-pHluorin (B) and pMep2<sup>H194E</sup>-pHluorin (C) and grown in a buffered minimal medium (pH = 6.1) containing 0.1% proline. Evolution of pH, corrected for negative control variations (delt...

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