<p>a. Luciferase reporter assays in VAL and OCI-LY7 cells expressing 4EBP1. Cells were electroporated with the dual luciferase reporter constructs and treated with 100 nM MLN0128 (+/−1 µg/ml Doxycycline) for 16 hrs. Results are averaged for three independent experiments and statistical significance measured using a t-test (*p<0.05, **p<0.01). b. Propidium iodide staining to measure the percentage of cells with sub-diploid DNA content in the VAL cells expressing 4EBP1 upon treatment with 100 nM MLN0128 with and without doxycycline induction. Results averaged for three independent experiments. Statistical analysis using a t-test (**p<0.01). c. Annexin V and PI staining to measure the percentage of cells undergoing apoptosis in the VAL cells expressing 4EBP1 upon treatment with 100 nM MLN0128 with and without doxycycline induction for 48 hours. Results averaged for three independent experiments. Statistical analysis using a t-test (**p<0.01). Shown to the right of the graphs is a western blot showing induction of 4EBP1 expression with 1 µg/ml Doxycycline.</p>