Image preprocessing.

<p>(A) The raw, unaligned, kymograph shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0121905#pone.0121905.g001" target="_blank">Fig 1a</a> (scale bar is 2 <i>μ</i>m, 20 seconds of imaging). (B) The kymograph after Gaussian smoothing, and (C) The Laplacian response imag... View More