Rapid Immunogen Selection (R.I.S.) method

(6103037), Carolina Beltran-Pavez; (6103040), Carolina B. Ferreira; (781674), Alberto Merino-Mansilla; (6103043), Amanda Fabra-Garcia; (6103046), Maria Casadella; (440725), Marc Noguera-Julian; (180297), Roger Paredes; (3343655), Alex Olvera; (2148178), Isabel Haro; (64220), Christian Brander; (5660647), Felipe Garcia; (5657728), Jose M. Gatell; (5664688), Eloisa Yuste; (5653459), Victor Sanchez-Merino

doi:10.1371/journal.pone.0208345.g001

Public

Rapid Immunogen Selection (R.I.S.) method.

Published in Figshare • Dec 19, 2018

NobleIDNI2P98W77R99S69

Authors:

Carolina Beltran-Pavez (6103037)

,

Carolina B. Ferreira (6103040)

,

Alberto Merino-Mansilla (781674)

Abstract

<p>(A) Random mutations were introduced into HIV-1 <i>env</i> AC10_29 sequence by mutagenic PCR. (B) A library of chimeric HIV virions was generated by cloning randomly mutated envelopes into pNL4-3 using XbaI and NotI restriction sites and transient transfection into 293T cells. (C) Virion library ...

Subject

Immunogen

Transfection

Cloning (programming)

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