This case study evaluates the use of organic diffusive gradients in thin-film (o-DGT) devices as proxies for measuring the bioavailability of methamphetamine and its metabolite amphetamine in zebrafish. The study critically analyzes the experimental design, quality assurance, and conclusions of Yin et al. (2019) , highlighting discrepancies between o-DGT-measured concentrations and actual zebrafish bioconcentration, particularly at higher exposure levels. While the study demonstrates correlations between o-DGT data and bioavailability, it reveals limitations in predictive accuracy due to differential behaviors of chemoavailability concentration factors and bioconcentration factors. The research underscores the potential of o-DGT as a tool for environmental monitoring but emphasizes the need for improved parameterization and standardized QA/QC protocols. Future directions include developing modeling frameworks to better relate o-DGT measurements to bioavailability, incorporating environmental variables, and validating methods in field conditions to enhance their applicability for organic pollutants.