National Museum of Japanese History

ABSTRACT Radiocarbon ( 14 C) ages cannot provide absolutely dated chronologies for archaeological or paleoenvironmental studies directly but must be converted to calendar age equivalents using a calibration curve compensating for fluctuations in atmospheric 14 C concentration. Although calibration curves are constructed from independently dated archives, they invariably require revision as new data become available and our understanding of the Earth system improves. In this volume the international 14 C calibration curves for both the Northern and Southern Hemispheres, as well as for the ocean surface layer, have been updated to include a wealth of new data and extended to 55,000 cal BP. Based on tree rings, IntCal20 now extends as a fully atmospheric record to ca. 13,900 cal BP. For the older part of the timescale, IntCal20 comprises statistically integrated evidence from floating tree-ring chronologies, lacustrine and marine sediments, speleothems, and corals. We utilized improved evaluation of the timescales and location variable 14 C offsets from the atmosphere (reservoir age, dead carbon fraction) for each dataset. New statistical methods have refined the structure of the calibration curves while maintaining a robust treatment of uncertainties in the 14 C ages, the calendar ages and other corrections. The inclusion of modeled marine reservoir ages derived from a three-dimensional ocean circulation model has allowed us to apply more appropriate reservoir corrections to the marine 14 C data rather than the previous use of constant regional offsets from the atmosphere. Here we provide an overview of the new and revised datasets and the associated methods used for the construction of the IntCal20 curve and explore potential regional offsets for tree-ring data. We discuss the main differences with respect to the previous calibration curve, IntCal13, and some of the implications for archaeology and geosciences ranging from the recent past to the time of the extinction of the Neanderthals.

Ancient migrations in Southeast Asia The past movements and peopling of Southeast Asia have been poorly represented in ancient DNA studies (see the Perspective by Bellwood). Lipson et al. generated sequences from people inhabiting Southeast Asia from about 1700 to 4100 years ago. Screening of more than a hundred individuals from five sites yielded ancient DNA from 18 individuals. Comparisons with present-day populations suggest two waves of mixing between resident populations. The first mix was between local hunter-gatherers and incoming farmers associated with the Neolithic spreading from South China. A second event resulted in an additional pulse of genetic material from China to Southeast Asia associated with a Bronze Age migration. McColl et al. sequenced 26 ancient genomes from Southeast Asia and Japan spanning from the late Neolithic to the Iron Age. They found that present-day populations are the result of mixing among four ancient populations, including multiple waves of genetic material from more northern East Asian populations. Science , this issue p. 92 , p. 88 ; see also p. 31

Anatomically modern humans reached East Asia more than 40,000 years ago. However, key questions still remain unanswered with regard to the route(s) and the number of wave(s) in the dispersal into East Eurasia. Ancient genomes at the edge of the region may elucidate a more detailed picture of the peopling of East Eurasia. Here, we analyze the whole-genome sequence of a 2,500-year-old individual (IK002) from the main-island of Japan that is characterized with a typical Jomon culture. The phylogenetic analyses support multiple waves of migration, with IK002 forming a basal lineage to the East and Northeast Asian genomes examined, likely representing some of the earliest-wave migrants who went north from Southeast Asia to East Asia. Furthermore, IK002 shows strong genetic affinity with the indigenous Taiwan aborigines, which may support a coastal route of the Jomon-ancestry migration. This study highlights the power of ancient genomics to provide new insights into the complex history of human migration into East Eurasia.

Abstract The color properties of aging wood samples from historical buildings have been compared with those of recent wood samples that were heat treated at temperatures ranging from 90°C to 180°C. The results of kinetic analysis obtained by the time-temperature superposition method showed that the color change during natural aging was mainly due to a slow and mild oxidation process. In other words, heat treatment could accelerate the changes in wood color that occur during aging. In one sample, the color change ( ΔE* ab ) after 921 years at ambient temperature was almost equivalent to that of heating (artificial aging) approximately for 6.7 h at 180°C. The results have been interpreted that the aging and the subsequent change in wood color begin at the time of tree harvesting.

Color is one of the most important features in digital images. The representation of color in digital form with a three-component image (RGB) is not very accurate, hence the use of a multiple-component spectral image is justified. At the moment, acquiring a spectral image is not as easy and as fast as acquiring a conventional three-component image. One answer to this problem is to use a regular digital RGB camera and estimate its RGB image into a spectral image by the Wiener estimation method, which is based on the use of a priori knowledge. In this paper, the Wiener estimation method is used to estimate the spectra of icons. The experimental results of the spectral estimation are presented.

Mitochondrial genetic variations were used to investigate the relationships between two Japanese wild boars, Japanese wild boar (Sus scrofa leucomystax) and Ryukyu wild boar (S.s. riukiuanus). Nucleotide sequences of the control (27 haplotypes) and cytochrome b (cyt-b) regions (19 haplotypes) were determined from 59 Japanese wild boars, 13 Ryukyu wild boars and 22 other boars and pigs. From phylogenetic analyses, the mtDNA of Ryukyu wild boar has a distinct lineage from that of Japanese wild boar, which was classified into the Asian pig lineage. This result suggests that the Ryukyu wild boar has a separate origin from the Japanese wild boar.

Abstract. Oxygen isotope ratios (δ18O) of tree-ring cellulose are a novel proxy for summer hydroclimate in monsoonal Asia. In central Japan, we collected 67 conifer wood samples, mainly Chamaecyparis obtusa, with ages encompassing the past 2600 years. The samples were taken from living trees, archeological wood, architectural wood, and buried logs. We analyzed stable isotope ratios of oxygen (δ18O) and hydrogen (δ2H) in tree-ring cellulose in these samples (more than 15 000 rings in total) without using a pooling method and constructed a statistically reliable tree-ring cellulose δ18O time series for the past 2500 years. However, there were distinct age trends and level offsets in the δ18O record, and cellulose δ18O values showed a gradual decrease as an individual tree matures. This suggested it is difficult to establish a cellulose δ18O chronology for low-frequency signals by simple averaging of all the δ18O time series data. In addition, there were opposite age trends in the cellulose δ2H, and δ2H gradually increased with tree age. There were clear positive correlations in the short-periodicity variations between δ18O and δ2H, probably indicating a common climate signal. A comparison of the δ18O and δ2H time series in individual trees with tree-ring width suggested that the opposite age trends of δ18O and δ2H are caused by temporal changes in the degree of post-photosynthetic isotope exchange with xylem water (physiological effect), accompanied by changes in stem growth rate that are influenced by human activity in the forests of central Japan. Based on the assumptions that cellulose δ18O and δ2H vary positively and negatively with constant proportional coefficients due to climatological and physiological effects, respectively, we solved simultaneous equations for the climatological and physiological components of variations in tree-ring cellulose δ18O and δ2H in order to remove the age trend. This enabled us to evaluate the climatic record from cellulose δ18O variations. The extracted climatological component in the cellulose δ18O for the past 2600 years in central Japan was well correlated with numerous instrumental, historical, and paleoclimatological records of past summer climate at various spatial and temporal scales. This indicates that integration of tree-ring cellulose δ18O and δ2H data is a promising method to reconstruct past summer climate variations on annual to millennial timescales, irrespective of the growth environment. However, analytical and statistical methods need to be improved for further development of this climate proxy.

Recently, primitive-type pottery was discovered in the Russian Far East, China, and Japan. Radiocarbon ages of far earlier than 10,000 BP have been obtained, relating directly or indirectly to the pottery. As an example of these very old 14 C ages for incipient pottery, we report here 14 C ages of charred adhesions on five potsherds and three charred wood fragments that were collected with the archeological artifacts (stone tools from the Chojakubo Culture) in the loam layers at the Odai Yamamoto I site (41°03′44′′N, 140°33′20′′E) in Aomori prefecture, at the northern end of the Japanese main island. The carbonaceous remains on the surface of the potsherds could be ancient food residues or soot from fuel for cooking. These small carbon samples were dated at the Tandetron accelerator mass spectrometry (AMS) 14 C dating facility at Nagoya University, as well as by Beta Analytic Co. Ltd. Except for two charred wood 14 C dates, 7070 ± 40 and 7710 ± 40 BP, all five charred-residue samples and one wood charcoal sample gave older 14 C ages of 12,680–13,780 BP, corresponding to the period of the Chojakubo Culture in Japan. This culture marks the beginning of the Jomon Culture, which is characterized by pottery usage and bow-and-arrow hunting.

Wood is present in many cultural heritage objects in Japan thanks to its capacity to resist over a long period of time. However, the evolution of its properties in regular use remains insufficiently known. The present study on the effect of wood aging takes advantage of the Japanese context where building traditions have been maintained for centuries. 3-point bending tests were performed in longitudinal (L) and radial (R) directions on small clear wood specimens cut from 8 historical samples and one modern reference considered of high quality by craftsmen. Although aged wood appeared more rigid and stronger than recent wood, after density and humidity corrections were applied no significant variation of L and R rigidity or L strength was observed. The post-linear behaviour, however, was drastically influenced by wood age especially in R direction where the strength and rupture energy decreased markedly with the time elapsed since the wood was processed. Well preserved aged wood considered as safe as long as it is not loaded perpendicular to grain.

Mitochondrial DNA (mtDNA) major non-coding regions were amplified from 73 dogs of eight Japanese native dog breeds and from 21 dogs of 16 non-Japanese dog breeds by the polymerase chain reaction and their DNA sequences were determined. A total of 51 nucleotide positions within the non-coding region (969-972 base pairs) showed nucleotide variations of which 48 were caused by transition. These nucleotide substitutions were abundant in the region proximate to tRNA(Pro). In addition to the nucleotide substitutions, the dog mtDNA D-loop sequences had a heteroplasmic repetitive sequence (TACACGTAGCG) involving size variation. The DNA sequences of the non-coding region were classified into four different groups by phylogenetic analysis and the deepest branchpoints of this dog phylogeny was calculated to about 100,000 years before the present. Phylogenetic analysis showed that Japanese native dog breeds could not be clearly delimited as distinct breeds. Many haplotypes found in members of some clustering groups were seen in each dog breed, and interbreed nucleotide differences between Japanese dog breeds were almost the same as the intrabreed nucleotide diversities.

This paper presents a specific examination of the introduction of grain cultivation and the processes of development in the Japanese Archipelago. In fact, no definitive archaeological evidence has been found that Jomon hunter–gatherers cultivated grain in the Japanese Archipelago; the earliest potential evidence of grain is a stamp mark of rice on the surface of a final late-Jomon, in about 11th century BC, pottery found at the Itaya 3 site in Shimane Prefecture. Current evidence indicates that the first grain cultivation was started by Jomon people who adopted irrigated wet rice cultivation that had arrived from the Korean Peninsula to northern parts of Kyushu, and gradually spread eastward thereafter. This study specifically examines four regions, including northern Kyushu, Kinki, southern Kanto, and northern Tohoku, in order to investigate the processes of grain cultivation initiation and spread. First, the years during which wet rice cultivation started in each region are estimated based on carbon-14 dating of earthenware types used during that period. Secondly, the timing of the spread of wet rice cultivation has been estimated based on carbon-14 dating of earthenware. Subsequently, differences in the periods between the initiation and dissemination of wet rice cultivation were estimated. Results suggest that dissemination took place over approximately 250 years in northern Kyushu, where wet rice cultivation first started. The time required for adoption decreased gradually as the trend moved eastward. It was estimated to have taken approximately 150 years in Kinki and 20–30 years in southern Kanto, taking place at about the same time. A factor, significantly contributing to such differences in timing and development processes among regions, was likely the relationship between the first farmers who introduced wet rice farming and the indigenous hunter–gatherers who lived there.

Abstract Annual rings record the intensity of cosmic rays (CRs) that had entered into the Earth’s atmosphere. Several rapid 14 C increases in the past, such as the 775 CE and 994CE 14 C spikes, have been reported to originate from extreme solar proton events (SPEs). Another rapid 14 C increase, also known as the ca. 660 BCE event in German oak tree rings as well as increases of 10 Be and 36 Cl in ice cores, was presumed similar to the 775 CE event; however, as the 14 C increase of approximately 10‰ in 660 BCE had taken a rather longer rise time of 3–4 years as compared to that of the 775 CE event, the occurrence could not be simply associated to an extreme SPE. In this study, to elucidate the rapid increase in 14 C concentrations in tree rings around 660 BCE, we have precisely measured the 14 C concentrations of earlywoods and latewoods inside the annual rings of Japanese cedar for the period 669–633 BCE. Based on the feature of 14 C production rate calculated from the fine measured profile of the 14 C concentrations, we found that the 14 C rapid increase occurred within 665–663.5 BCE, and that duration of 14 C production describing the event is distributed from one month to 41 months. The possibility of occurrence of consecutive SPEs over up to three years is offered.

Abstract The so-called Millennium Eruption of Baitoushan Volcano is one of the largest of the Common Era but its date has been uncertain. Recently, Oppenheimer et al. (2017) reported the eruptive year as late AD 946 using a new method called carbon-14 spike matching. However, it is necessary to verify their result to confirm the eruptive year, since only one wood sample was used in their study. We verified the eruptive year by measuring 14 C contents in tree rings from another wood sample buried during the Baitoushan eruption. We succeeded in reproducing the AD 774–775 14 C spike (Miyake et al. 2012), and counted the number of rings from the outermost ring accompanied by bark to the ring possessing the AD 774–775 14 C spike. We found the outermost ring was formed in AD 946. Our study supported the result of Oppenheimer et al. (2017), which makes the eruptive year conclusive. Also, we suggest that the 14 C spike-matching method can be a prominent dating tool applicable to ancient woods that are difficult to date using the usual dendrochronology techniques.

Elemental analysis of materials is fundamentally important to science and technology. Many elemental analysis methods have been developed, but three-dimensional nondestructive elemental analysis of bulk materials has remained elusive. Recently, our project team, dreamX (damageless and regioselective elemental analysis with muonic X-rays), developed a nondestructive depth-profiling elemental analysis method after a decade of research. This new method utilizes a new type of probe; a negative muon particle and high-energy muonic X-rays emitted after the muon stops in a material. We performed elemental depth profiling on an old Japanese gold coin (Tempo-Koban) using a low-momentum negative muon beam and successfully determined that the Au concentration in the coin gradually decreased with depth over a micrometer length scale. We believe that this method will be a promising tool for the elemental analysis of valuable samples, such as archeological artifacts.

Abstract Quantitative elemental analysis method of a bulk material by examining the relation between intensity of muonic X-rays and elemental composition was developed. Using this new calibration method, we demonstrated quantitatively determination of the elemental composition inside of an ancient Chinese coin without sample destruction.

Whether man is predisposed to lethal violence, ranging from homicide to warfare, and how that may have impacted human evolution, are among the most controversial topics of debate on human evolution. Although recent studies on the evolution of warfare have been based on various archaeological and ethnographic data, they have reported mixed results: it is unclear whether or not warfare among prehistoric hunter-gatherers was common enough to be a component of human nature and a selective pressure for the evolution of human behaviour. This paper reports the mortality attributable to violence, and the spatio-temporal pattern of violence thus shown among ancient hunter-gatherers using skeletal evidence in prehistoric Japan (the Jomon period: 13 000 cal BC-800 cal BC). Our results suggest that the mortality due to violence was low and spatio-temporally highly restricted in the Jomon period, which implies that violence including warfare in prehistoric Japan was not common.

Abstract Blooming seasonality is an important trait in ornamental plants and was selected by humans. Wild roses flower only in spring whereas most cultivated modern roses can flower continuously. This trait is explained by a mutation of a floral repressor gene, RoKSN , a TFL1 homologue. In this work, we studied the origin, the diversity and the selection of the RoKSN gene. We analyzed 270 accessions, including wild and old cultivated Asian and European roses as well as modern roses. By sequencing the RoKSN gene, we proposed that the allele responsible for continuous-flowering, RoKSN copia , originated from Chinese wild roses ( Indicae section), with a recent insertion of the copia element. Old cultivated Asian roses with the RoKSN copia allele were introduced in Europe, and the RoKSN copia allele was progressively selected during the 19th and 20th centuries, leading to continuous-flowering modern roses. Furthermore, we detected a new allele, RoKSN A181 , leading to a weak reblooming. This allele encodes a functional floral repressor and is responsible for a moderate accumulation of RoKSN transcripts. A transient selection of this RoKSN A181 allele was observed during the 19th century. Our work highlights the selection of different alleles at the RoKSN locus for recurrent blooming in rose.

In this paper, the morphologies of martensite and retained austenite for 1.5 mass%C-12 mass%Cr and 1 mass%C-8 mass%Cr steels were observed by means of optical microscopy, XRD, SEM/EBSD and TEM. The amount of retained austenites was quantitatively investigated and compared with XRD, EBSD and TEM observation methods. The retained austenites were distributed in the form of a block type and a film type in the martensite structures. For the 1.5 mass%C-12 mass%Cr steel, the amount of block type retained austenite and film type one are almost equal in three distinct regions of carbide; primary carbide, secondary carbide and without carbide. In the case of 1 mass%C-8 mass%Cr steel, as to film type retained austenite there are not so much differences among those regions. However, block type retained austenites are distributed with much amount, especially in the region around primary carbide.

The radiocarbon content of Japanese cedars was measured by accelerator mass spectrometry for decadal tree-ring samples from the period of 240 BC to AD 900. Conventional gas counting was also used for part of the samples. The data were compared with the INTCAL98 calibration curve (Stuiver et al. 1998). The results indicate that the difference in atmospheric 14 C between Japan and North America or Europe is negligible at this period, less than 18 14 C yr using an average of 50 yr. However, in the period of about AD 100 to about AD 200, we cannot exclude the possibility of a deviation of the order of 30 to 40 14 C yr to the older ages.

Muon irradiation and muonic X-ray detection can be applied to non-destructive elemental analysis. In this study, in order to develop the elemental analysis by muonic X-ray measurement we constructed a new X-ray measuring system in J-PARC muon facility. We performed muon irradiation for Tempo-koban (Japanese old coin) for test experiment of elemental analysis. Muonic X-rays originating from muon transition in muonic silver and gold atoms were identified. The contents of Tempo-koban (Au:56%) was determined by muonic X-ray intensities.