Observatorio Astronómico Los Molinos

Timed artificial insemination (TAI) is a very important tool in production systems, as it has a direct impact on cost-efficiency by reducing labour resulting from oestrus detection. However, to make TAI commercially feasible, hormonal treatments need to assure acceptable pregnancy rates and be economically viable. Recent advances in the knowledge of ovarian physiology (e.g. determination of follicular waves, sensitivity of the early corpus luteum) in small ruminants allowed the development of new treatments focused on an efficient estimation and synchronisation of the time of ovulation of the females of a treated flock/herd. In this review we summarise new information and concepts in the hormonal control of the life span of the corpus luteum, as well as methods to manipulate follicular growth in small ruminants. Additionally, we elaborate on recent studies concerning the use of TAI associated with short progestogen treatment in goats and the newly developed Synchrovine™ protocol (two doses of prostaglandin F2a given seven days apart) in sheep.

This article reviews four 'DNA screening techniques', namely heteroduplex analysis, single-strand conformational polymorphism (SSCP), denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) as tools for the study of allelic variation in natural populations. The resolving power, advantages, and limitations of each technique are discussed and compared. We also provide some criteria for choosing among techniques and illustrate some practical issues with examples taken primarily from our own laboratory experience.

Abstract Early differentiation in rainbow trout gonads was investigated by expression profiling and in situ hybridization (ISH). Expression of cyp19a1 and fst in females and sox9a1 in males were sexually dimorphic between 32 to 35 days post‐fertilization (dpf). After 35 dpf, the differentiation proceeded with sexually dimorphic profiles for sox9a2, dmrt1, cyp11b2.1, amh in males and foxl2a, foxl2b, hsd3b1, inha in females. cyp17a1, cyp11a1, star, nr5a1b increased only after 40 dpf in both sexes with a slightly higher expression in females. cyp19a1 expression was localized in a cluster of somatic cells in the ventral side of female gonads, and sox9a2 and amh in somatic cells surrounding the germ cells, at 28 dpf and thereafter, both in male and female gonads. cyp11b2.1, cyp17a1 , and cyp11a1 expressions were only detected in scattered somatic cells in males after 46 dpf. This confirms the early implication of cyp19a1 in trout ovarian differentiation and suggests that early testicular differentiation does not need androgen production. Developmental Dynamics 236:2198–2206, 2007. © 2007 Wiley‐Liss, Inc.

Timed artificial insemination (TAI) is a very important tool in production systems, as it has a direct impact on cost-efficiency by reducing labour resulting from oestrus detection. However, to make TAI commercially feasible, hormonal treatments need to assure acceptable pregnancy rates and be economically viable. Recent advances in the knowledge of ovarian physiology (e.g. determination of follicular waves, sensitivity of the early corpus luteum) in small ruminants allowed the development of new treatments focused on an efficient estimation and synchronisation of the time of ovulation of the females of a treated flock/herd. In this review we summarise new information and concepts in the hormonal control of the life span of the corpus luteum, as well as methods to manipulate follicular growth in small ruminants. Additionally, we elaborate on recent studies concerning the use of TAI associated with short progestogen treatment in goats and the newly developed Synchrovine protocol (two doses of prostaglandin F(2alpha) given seven days apart) in sheep.

The aim of this study was to evaluate the prevalence and severity of temporomandibular disorders (TMD) in Brazilian college students. A questionnaire was administered to 2,396 students. Seventy-three percent of women (mean age 21.94 +/- 5 years) and 27% of men (mean age 22.41 +/- 4.8 years) answered the questionnaire. The anamnestic index was used to classify the volunteers according to TMD severity degree. The results showed a higher percentage of men without TMD (43.74%) (p < 0.05, Chi-square test). The women exhibited some degree of severity (73.03%) at a higher frequency than men (56.26%). No significant differences were observed between sexes for a same TMD severity degree (p > 0.05). The results indicated TMD prevalence in Brazilian college students similar to that presented in other studies found in the literature reviewed. Longitudinal studies are recommended to follow the prevalence and health care needs in this population.

This study tested whether the effects of a short period of nutritional supplementation given to ewes during the luteal phase on follicle development and ovulation rate is associated with an increase in circulating concentrations of FSH, glucose or metabolic hormones. Oestrus was synchronised with two prostaglandin injections given 9 days apart and the supplement consisted of corn grain and soybean meal. Corriedale ewes with low body condition were randomly assigned to 2 groups: the control group (C; n = 10) received a maintenance diet while the short-term supplemented group (STS; n = 10) received double the maintenance diet over days 9 to 14 of the oestrous cycle (day 0 = ovulation). Ovaries were examined daily by ultrasound and blood was sampled three times a day during the inter-ovulatory interval for measuring reproductive and metabolic hormones. On days 9, 11 and 14 of the oestrous cycle, half of the ewes from each group (n = 5) were bled intensively to determine the concentrations of glucose, insulin, IGF-I and leptin. Plasma FSH, progesterone, oestradiol and androstenedione concentrations were similar among groups. Dietary supplementation increased plasma insulin concentrations from the first to the sixth day of supplementation and increased glucose concentrations on the third day, compared with control ewes. Plasma leptin concentrations were higher in STS ewes from the second to the fifth day of supplementation. The pattern of IGF-I concentrations was similar among groups. In STS ewes, the nutritional treatment prolonged the lifespan of the last non-ovulatory follicle, so fewer follicular waves developed during the cycle. In STS ewes, increased concentrations of glucose, insulin and leptin one day before ovulatory wave emergence were associated with increased numbers of follicles growing from 2 to 3 mm and with stimulation of the dominant follicle to grow for a longer period. We suggest that the mechanism by which short-term nutritional supplementation affects follicle development does not involve an increase in FSH concentrations, but may involve responses to increased concentrations of glucose, insulin and leptin, acting directly at the ovarian level. This effect is acute, since concentrations of all three substances decrease after reaching peak values on the third day of supplementation. The status of follicle development at the time of maximum concentrations of glucose and metabolic hormones may be one of the factors that determines whether ovulation rate increases or not.

The present study was designed to obtain new insights into fish gonadal sex differentiation by comparing the effects of two different masculinizing treatments on some candidate gene expression profiles. Masculinization was induced in rainbow trout, Oncorhynchus mykiss, genetic all-female populations using either an active fish androgen (11betaAnd, 11beta-hydroxyandrostenedione) or an aromatase inhibitor (ATD, 1,4,6-androstatriene-3,17-dione). The expression profiles of 100 candidate genes were obtained by real-time RT-PCR, and 46 profiles displayed a significant differential expression between control populations (males and females) and ATD/11betaAnd-treated populations. These expression profiles were grouped in four temporally correlated expression clusters. Among the common responses shared by the two masculinizing treatments, the inhibition of some early female differentiating genes (cyp19a1, foxl2a, fst, and fshb) appears to be crucial for effective masculinization, suggesting that these genes act together via a short regulation loop to maintain high sex-specific ovarian expression of cyp19a1. This simultaneous down-regulation of female-specific genes could be triggered by some testicular genes, such as dmrt1, nr0b1 (also known as dax1), and pdgfra, which are quickly up-regulated by the two masculinizing treatments. In contrast to 11betaAnd, ATD quickly restored the expression levels of steroidogenesis related genes (cyp11b2.1, cyp11b2.2, hsd3b1, cyp17a, star, and nr5a1) and some Sertoli cell markers (sox9a2 and amh) to the expression levels observed during control testicular differentiation. This demonstrates that these genes are probably not needed for active masculinization and that the inhibition of endogenous estrogen synthesis produces a much more complete and specific testicular pattern of gene expression than that observed following androgen-induced masculinization.

Abstract Two hundred and seventy-seven ewes were used in three experiments during late seasonal anoestrus to evaluate short-term progestogen primings for equine chorionic gonadotropin (eCG)-induced oestrus. In experiment 1, medroxy-progesterone acetate (MAP) sponges were inserted for 6, 9 or 13 days in nulliparous ewes. Percentage of ewes in oestrus (92.3, 85.7 and 96.4%, respectively) and conception rates (66.7, 75.0 and 63.0%, respectively) did not differ among groups (T &gt; 0·05). In experiment 2, MAP sponges were inserted for 1, 2, 3, 6 or 12 days in multiparous ewes. Percentages of ewes in oestrus were higher with 3, 6 or 12 days than with 1 or 2 days of priming (72.2, 93.8 and 87.5% v . 25.0 and 33.3% respectively, P &lt; 0.05) and maximum response was with 6-day priming. From both experiments we conclude that short-term progestogen (6 days) priming is as effective as long-term traditional primings for inducing oestrus. In experiment 3, level of progestogen priming was compared among treatments using a new progesterone releasing device (CIDR) for 6 days, a 6-day used CIDR for 6 days, or an 11-day used CIDR for 6 days. While percentages of oestrous response were similar among groups (95.9, 93.6 and 88.9%, respectively), pregnancy rates were higher with a new CIDR than with one that had been used for 11 days (57.1% v . 27.8%, P &lt; 0.05), with the 6-day used CIDR being intermediate (51.1%). We conclude that a 6-day progestogen priming combined with eCG can be used successfully to induce fertile oestrus in seasonal anoestrous ewes if progestogen levels during priming are kept sufficiently high.

Americanae nace como un proyecto conjunto que surge dentro de la Red Europea de Información y Documentación sobre América Latina (REDIAL), y que ha afrontado la Biblioteca de la Agencia Española de Cooperación Internacional para el Desarrollo (AECID). Esta nueva biblioteca virtual hace más accesibles los libros digitales de tema americanista a los investigadores y usuarios interesados de cualquier parte del mundo.

In non-mammalian vertebrates, estrogens are key players in ovarian differentiation, but the mechanisms by which they act remain poorly understood. The present study on rainbow trout was designed to investigate whether estrogens trigger the female pathway by activating a group of early female genes (i.e. cyp19a1, foxl2a, foxl2b, fst, bmp4, and fshb) and by repressing early testicular markers (i.e. dmrt1, nr0b1, sox9a1 and sox9a2). Feminization was induced in genetically all-male populations using 17alpha-ethynylestradiol (EE2, 20 mg/kg of food during 2 months). The expression profiles of 100 candidate genes were obtained by real-time RT-PCR and 45 expression profiles displayed a significant differential expression between control populations (males and females) and EE2-treated populations. These expression profiles were grouped in five temporally correlated expression clusters. The estrogen treatment induced most of the early ovarian differentiation genes (foxl2a, foxl2b, fst, bmp4, and fshb) and in particular foxl2a, which was strongly and quickly up-regulated. Simultaneously, Leydig cell genes, involved in androgen synthesis, as well as some Sertoli cell markers (amh, sox9a2) were strongly repressed. However, in contrast to our initial hypothesis, some genes considered as essential for mammalian and fish testis differentiation were not suppressed during the early process of estrogen-induced feminization (dmrt1, nr0b1, sox9a1 and pax2a) and some were even strongly up-regulated (nr0b1, sox9a1and pax2a). In conclusion, estrogens trigger male-to-female transdifferentiation by up-regulating most ovarian specific genes and this up-regulation appears to be crucial for an effective feminization, but estrogens do not concomitantly down-regulate all the testicular differentiation markers.

I review studies on decapod crustaceans to draw conclusions about the importance of effects of past environmental conditions on development, phenotype, performance, and survival in animals. I consider 3 critical points of the life cycle: the allocation of reserves into eggs, the hatching of larvae, and metamorphosis from the larval to the juvenile phase. Biomass allocated to eggs varies among females as a response to changes in environmental conditions. These variations are propagated to the larval stages, influencing the biomass at hatching, subsequent larval developmental pathways, and survival during periods of limited starvation. Suboptimal conditions experienced by embryos increase the loss of mass during embryogenesis; size or biomass of the juvenile is either positively or negatively correlated with initial biomass. Positive correlations may be the normal pattern; negative correlations occur when individuals hatched with low initial biomass follow developmental pathways that lead to increased biomass at metamorphosis. In estuarine crabs, salinity experienced by embryos leads to salinity acclimation in early larval stages. Phenotypic links originate as transgenerational effects that propagate to the juvenile stages. There are least 3 types of effects: disruption of physiological processes; direct adaptive responses; and indirect consequences of adaptive mechanisms. All types appear within a species; they are produced as a response to a single environmental factor. Variability in phenotype remains latent and is expressed in terms of survival according to the environmental conditions experienced by a particular stage. The fate of individuals is thus affected by interactions between their immediate developmental processes and their environmental history.

The relationship between nutrition and reproduction in sheep has been the subject of research in several international groups. This review will particularly focus on the effects of undernutrition on the potential causes of reproductive failure including abnormalities of the ovum or the embryo, luteal inadequacy and failure of the supply of progesterone to the uterus, or the mechanisms involved in maternal recognition of pregnancy. The level of nutrition and peripheral progesterone concentrations are inversely related, and increased rates of embryo loss, associated with higher progesterone concentrations in ewes with low levels of nutrition have been reported. Undernutrition may act through changes in the distribution of progesterone in the endometrium. Thus, lower endometrial levels on day 5 of the cycle in ewes fed half of their maintenance requirements have been observed, providing a link between the known role of progesterone in embryo survival by the modulation of uterine function and the higher embryo losses found in undernourished ewes. The evidence of an effect of maternal nutrition on IFNtau secretion from the conceptus and of PGF2alpha production from the uterus is presented. Moreover, undernutrition provokes a reduction in the sensitivity of the endometrium to progesterone that may affect embryo survival. Finally, a state of undernutrition induces changes in the endometrial sensitivity to steroid hormones at early stages of pregnancy that could adversely alter uterine environment to the detriment of embryo survival.

We determine the extinction curve in the z_l=0.83 lens galaxy of the gravitational lens SBS0909+532 from the wavelength dependence of the flux ratio between the lensed quasar images (z_s=1.38) from 3400 to 9200\AA. It is the first measurement of an extinction curve at a cosmological distance of comparable quality to those obtained within the Galaxy. The extinction curve has a strong 2175\AA feature, a noteworthy fact because it has been weak or non-existent in most estimates of extinction curves outside the Galaxy. The extinction curve is fitted well by a standard $R_V=2.1\pm0.9$ Galactic extinction curve. If we assume standard Galactic extinction laws, the estimated dust redshift of $z=0.88\pm0.02$ is in good agreement with the spectroscopic redshift of the lens galaxy. The widespread assumption that SMC extinction curves are more appropriate models for cosmological dust may be incorrect.

A catalog of a sample of 105 Jupiter family (JF) comets (defined as those with Tisserand constants and orbital periods yr) is presented with our “best estimates” of their absolute nuclear magnitudes . The catalog includes all the nuclear magnitudes reported after 1950 until August 1998 that appear in the International Comet Quarterly Archive of Cometary Photometric Data, the Minor Planet Center (MPC) data base, IAU Circulars, International Comet Quarterly, and a few papers devoted to some particular comets, together with our own observations. Photometric data previous to 1990 have mainly been taken from the Comet Light Curve Catalogue (CLICC) compiled by Kamél (1991). We discuss the reliability of the reported nuclear magnitudes in relation to the inherent sources of errors and uncertainties, in particular the coma contamination often present even at large heliocentric distances. A large fraction of the JF comets of our sample indeed shows various degrees of activity at large heliocentric distances, which is correlated with recent downward jumps in their perihelion distances. The reliability of coma subtraction methods to compute the nuclear magnitude is also discussed. Most absolute nuclear magnitudes are found in the range , with no magnitudes fainter than . The catalog can be found at: http://www.fisica.edu.uy/ ~gonzalo/catalog/.

Granular materials of different sizes are present on the surface of several atmosphereless Solar system bodies. The phenomena related to granular materials have been studied in the framework of the discipline called granular physics, both experimentally and, over the last few decades, by numerical simulations. The discrete element method simulates the mechanical behaviour of a medium formed by a set of particles which interact through their contact points. The difficulty in reproducing vacuum and low-gravity environments makes numerical simulations the most promising technique in the study of granular media under these conditions. In this work, relevant processes in minor bodies of the Solar system are studied using the discrete element method. Results of simulations of size segregation in low-gravity environments in the cases of the asteroids Eros and Itokawa are presented. The segregation of particles with different densities was analysed, in particular, the case of comet P/Hartley 2. The surface shaking in these different gravity environments could produce the ejection of particles from the surface at very low relative velocities. The shaking causing the above processes is due to impacts and explosions such as the release of energy by the liberation of internal stresses or the re-accommodation of material. Simulations of the passage of impact-induced seismic waves through a granular medium were also performed. We present several applications of the discrete element methods for the study of the physical evolution of agglomerates of rocks under low-gravity environments.

Abstract— On September 15, 2007, a bright fireball was observed and a big explosion was heard by many inhabitants near the southern shore of Lake Titicaca. In the community of Carancas (Peru), a 13.5 m crater and several fragments of a stony meteorite were found close to the site of the impact. The Carancas event is the first impact crater whose formation was directly observed by several witnesses as well as the first unambiguous seismic recording of a crater‐forming meteorite impact on Earth. We present several lines of evidence that suggest that the Carancas crater was a hypervelocity impact. An event like this should have not occurred according to the accepted picture of stony meteoroids ablating in the Earth's atmosphere, therefore it challenges our present models of entry dynamics. We discuss alternatives to explain this particular event. This emphasizes the weakness in the pervasive use of “average” parameters (such as tensile strength, fragmentation behavior and ablation behavior) in current modeling efforts. This underscores the need to examine a full range of possible values for these parameters when drawing general conclusions from models about impact processes.

Traditional treatments of two prostaglandin F2 alpha (PGF2alpha) doses at 10-day intervals or more did not result in acceptable pregnancy rates in timed artificial insemination (TAI) programmes in ewes. An explanation might be the undefined time-period of the onset of oestrus and ovulation after the treatment. Recently a consistent interval to oestrus and ovulation was obtained by giving PGF2alpha at day 3 post-ovulation, i.e. when the largest follicle of the first follicular wave of the cycle was still growing. This can be achieved when a second dose of PGF2alpha is given 7 days after a first dose. In this work, we evaluated the synchronization of oestrus and determined which of three different moments of TAI was the most successful using a PGF2alpha (PG-7d) protocol in a large flock. A total of 436 nulliparous and multiparous ewes were treated with two doses of a PGF2alpha analogue (delprostenate 160 microg, i.m.) separated by 7 days. Onset of oestrus was recorded twice a day and a single cervical TAI with fresh undiluted semen was performed either at 42 h (n = 152), 48 h (n = 120), or 54 h (n = 164), after the second PGF2alpha dose without taking into account the oestrous response. Pregnancy rate was determined by transrectal ultrasonography 30 days after insemination. Onset of oestrus was detected in 308 of 328 and 89 of 108 multiparous and nulliparous ewes, respectively (p < 0.001), within 72 h after treatment. The distribution of the onset of oestrus did not differ between multiparous and nulliparous ewes and the highest proportion of ewes in oestrus was detected between 25 to 48 h (313/397) from the second PGF2alpha dose. The pregnancy rate in ewes inseminated at 42 h tended to be higher than those inseminated at 48 h (p = 0.09) and was higher than those inseminated at 54 h (p < 0.05) (56/152, 31/120, 37/164; respectively). Therefore, the use of the PG-7d protocol resulted in a very high synchronization of oestrus with the highest concentration (around 80%) between 25 to 48 h from the end of treatment. The best pregnancy rate (37%) was obtained after a single cervical TAI with fresh semen at 42 h.

The fireball producing a crater‐forming meteorite fall near Carancas, Peru, on 15 September 2007 has been analyzed using eyewitness, seismic, and infrasound records. The meteorite impact, which produced a crater of 13.5 m diameter, is found to have released of order 10 10 J of energy, equivalent to ∼2–3 tons of TNT high explosives based on infrasonic measurements. Our best fit trajectory solution places the fireball radiant at an azimuth of 82° relative to the crater, with an entry angle from the horizontal of 63°. From entry modeling and infrasonic energetics constraints, we find an initial energy for the fireball to be in the 0.06–0.32 kton TNT equivalent. The initial velocity for the meteoroid is restricted to be below 17 km/s from orbit considerations alone, while modeling suggests an even lower best fit velocity close to 12 km/s. The initial mass of the meteoroid is in the range of 3–9 tons. At impact, modeling suggests a final end mass of order a few metric tons and impact velocity in the 1.5–4 km/s range. We suggest that the formation of such a substantial crater from a chondritic mass was the result of the unusually high strength (and corresponding low degree of fragmentation in the atmosphere) of the meteoritic body. Additionally, the high altitude of the impact site (3800 m.a.s.l) resulted in an almost one order of magnitude higher impact speed than would have been the case for the same body impacting close to sea level.

As a consequence of the combined effects of prey patchiness and diel or tidal vertical migrations in the water column, decapod crustacean larvae may experience temporal or spatial variability in the availability of planktonic food. In a laboratory study, we evaluated effects of temporarily limited access to prey on the larvae of three species of brachyuran crabs, Chasmagnathus granulata, Cancer pagurus and Carcinus maenas. Stage-I zoeae were fed ad libitum for 4 or 6 h per day (20 or 25% treatments; 6 h tested in C. pagurus only), and rates of larval survival and development were compared with those observed in continuously fed control groups (24 h, 100%). In C. granulata, we also tested if intraspecific variability in initial biomass of freshly hatched larvae originating from different broods has an influence on early larval tolerance of food limitation. Moreover, we exposed embryos and larvae of this estuarine species to moderately decreased salinities to identify possible interactions of osmotic and nutritional stress. Finally, we evaluated in this species the effect of food limitation on survival from hatching through all larval instars to metamorphosis. In all three species, limited access to prey had only weak or insignificant negative effects on survival through the Zoea-I stage. The strength of the effects of temporary food limitation varied in C. granulata significantly among broods. However, no significant relationships were found between initial larval biomass (C content) and either survival or development duration. Strongly decreased survival to metamorphosis was found when food limitation continued throughout larval development. Thus, early brachyuran crab larvae are well adapted to transitory lack of planktonic food. The capability of the Zoea-I stage of C. granulata to withstand nutritional stress also under conditions of concomitant salinity stress allows them to exploit various brackish environments within estuarine gradients. However, continued exposure to limited access to planktonic prey may exceed the nutritional flexibility of C. granulata larvae.

We present VR observations of QSO 2237+0305 conducted by the GLITP collaboration from 1999 October 1 to 2000 February 3. The observations were made with the 2.56 m Nordic Optical Telescope at Roque de los Muchachos Observatory, La Palma (Spain). The PSF fitting method and an adapted version of the ISIS subtraction method have been used to derive the VR light curves of the four components (A-D) of the quasar. The mean errors range in the intervals 0.01-0.04 mag (PSF fitting) and 0.01-0.02 mag (ISIS subtraction), with the faintest component (D) having the largest uncertainties. We address the relatively good agreement between the A-D light curves derived using different filters, photometric techniques, and telescopes. The new VR light curves of component A extend the time coverage of a high magnification microlensing peak, which was discovered by the OGLE team.