Application of RT-PCR designed from the sequence of the local SRSV strain to the screening in viral gastroenteritis outbreaks.

Saito, H; Saito, S; Kamada, K; Harata, S; Sato, H; Morita, M; Miyajima, Y

doi:10.1111/j.1348-0421.1998.tb02307.x

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Application of RT-PCR designed from the sequence of the local SRSV strain to the screening in viral gastroenteritis outbreaks.

Published in Microbiology and immunology • Jan 1, 1998

Authors:

H Saito

S Saito

K Kamada

Abstract

The Yuri strain of small round structured virus (SRSV) was cloned from a fecal specimen in which virus particles were observed by electron microscopy. The most common RT-PCR protocol in Japan, however, using 35/36 and NV81/NV82/SM82 nested primer pairs, could not detect the SRSV genome in this speci...

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