びんの切り方あれこれ(化学実験虎の巻 : 便利な実験器具・道具)
Published in Europe PMC (PubMed Central) • Dec 20, 1993
Authors:
利安 義雄
Abstract
We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-sal...
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