Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250

Neuhoff, Volker; Arold, Norbert; Taube, Dieter; Ehrhardt, Wolfgang

doi:10.1002/elps.1150090603

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Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250

Shared by NobleBlocks on Jan 1, 1988 • 12:00 AM UTC

Authors:

Volker Neuhoff

Norbert Arold

Dieter Taube

Abstract

Abstract An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G‐250 and R‐250. The new method is based on addition of 20 % v/v methanol and higher concentrations of ammonium sulfate...

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