Focusing of alkaline proteases (subtilisins) in pH 10–12 immobilized gradients

Bossi, Alessandra; Giorgio Righetti, Pier; Vecchio, Giuseppe; Severinsen, Steen

doi:10.1002/elps.11501501221

Public

Focusing of alkaline proteases (subtilisins) in pH 10–12 immobilized gradients

Shared by NobleBlocks on Jan 1, 1994 • 12:00 AM UTC

Authors:

Alessandra Bossi

Pier Giorgio Righetti

Giuseppe Vecchio

Abstract

Isoelectric focusing in very alkaline immobilized pH gradients (IPG) was adopted for checking the purity and assessing the pI value of two strongly alkaline proteases: Savinase and Durazym. The first enzyme (known to be the most alkaline) contains 5 Asp, 5 Glu, 7 His, 7 Tyr, 5 Lys, no Cys and 8 Arg ...

Subject

Alkaline protease

Proteases

Chemistry

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