Cell proliferation at 122°C and isotopically heavy CH
                    <sub>4</sub>
                    production by a hyperthermophilic methanogen under high-pressure cultivation

Takai, Ken; Nakamura, Kentaro; Toki, Tomohiro; Tsunogai, Urumu; Miyazaki, Masayuki; Miyazaki, Junichi; Hirayama, Hisako; Nakagawa, Satoshi; Nunoura, Takuro; Horikoshi, Koki

doi:10.1073/pnas.0712334105

Public

Cell proliferation at 122°C and isotopically heavy CH <sub>4</sub> production by a hyperthermophilic methanogen under high-pressure cultivation

Shared by NobleBlocks on Aug 5, 2008 • 12:00 AM UTC

Authors:

Ken Takai

Kentaro Nakamura

Tomohiro Toki

Abstract

We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types of deep-sea chemolithoautotrophs, including methanogens. It is based on a glass-syringe-sealing liquid medium and gas mixture used in conjunction wi...

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