Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities.

LD, Graham; KD, Haggett; PA, Jennings; Brocque DS, Le; RG, Whittaker; PA, Schober

doi:10.1021/bi00075a019

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Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities.

Shared by NobleBlocks on Jun 1, 1993 • 12:00 AM UTC

Authors:

Graham LD

Haggett KD

Jennings PA

Abstract

In the past, several point mutations have been introduced individually into the substrate-binding site of alpha-lytic protease (EC 3.4.21.12) and shown to affect its specificity in a predictable manner [Bone, R., Silen, J. L., & Agard, D. A. (1989) Nature 339, 191-195]. One of the resulting mutant e...

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