Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase: in vitro evidence for the NR-dependent formation of active nitrogen species.

H, Yamasaki; Y, Sakihama

doi:10.1016/s0014-5793(00)01203-5

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Simultaneous production of nitric oxide and peroxynitrite by plant nitrate reductase: in vitro evidence for the NR-dependent formation of active nitrogen species.

Shared by NobleBlocks on Feb 1, 2000 • 12:00 AM UTC

Authors:

Yamasaki H

Sakihama Y

Abstract

We examined the ability of plant nitrate reductase (NR) to produce nitric oxide (NO) using in vitro assays. Electrochemical and fluorometric measurements both showed that NO is produced by corn NR in the presence of nitrite and NADH at pH 7. The NO production was inhibited by sodium azide, a known i...

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