Fully validated LC-MS/MS assay for the simultaneous quantitation of coadministered therapeutic antibodies in cynomolgus monkey serum.

H, Jiang; J, Zeng; C, Titsch; K, Voronin; B, Akinsanya; L, Luo; H, Shen; DD, Desai; A, Allentoff; AF, Aubry; BS, Desilva; ME, Arnold

doi:10.1021/ac402420v

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Fully validated LC-MS/MS assay for the simultaneous quantitation of coadministered therapeutic antibodies in cynomolgus monkey serum.

Shared by NobleBlocks on Sep 26, 2013 • 12:00 AM UTC

Authors:

Jiang H

Zeng J

Titsch C

Abstract

An LC-MS/MS assay was developed and fully validated for the simultaneous quantitation of two coadministered human monoclonal antibodies (mAbs), mAb-A and mAb-B of IgG4 subclass, in monkey serum. The total serum proteins were digested with trypsin at 50 °C for 30 min after methanol denaturation and p...

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