Arginyl-tRNA synthetase from Escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics.

X Lin, S; P Shi, J; D Cheng, X; L Wang, Y

doi:10.1021/bi00417a022

Public

Arginyl-tRNA synthetase from Escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics.

Shared by NobleBlocks on Aug 23, 1988 • 12:00 AM UTC

Authors:

S X Lin

J P Shi

X D Cheng

Abstract

A Blue Sephadex G-150 affinity column adsorbs the arginyl-tRNA synthetase of Escherichia coli K12 and purifies it with high efficiency. The relatively low enzyme content was conveniently purified by DEAE-cellulose chromatography, affinity chromatography, and fast protein liquid chromatography to a p...

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