Broadly Reactive and Highly Sensitive Assay for Norwalk-Like Viruses Based on Real-Time Quantitative Reverse Transcription-PCR

Kageyama, Tsutomu; Kojima, Shigeyuki; Shinohara, Michiyo; Uchida, Kazue; Fukushi, Shuetsu; B. Hoshino, Fuminori; Takeda, Naokazu; Katayama, Kazuhiko

doi:10.1128/jcm.41.4.1548-1557.2003

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Broadly Reactive and Highly Sensitive Assay for Norwalk-Like Viruses Based on Real-Time Quantitative Reverse Transcription-PCR

Shared by NobleBlocks on Apr 1, 2003 • 12:00 AM UTC

Authors:

Tsutomu Kageyama

Shigeyuki Kojima

Michiyo Shinohara

Abstract

ABSTRACT We have developed an assay for the detection of Norwalk-like viruses (NLVs) based on reverse transcription-PCR (RT-PCR) that is highly sensitive to a broad range of NLVs. We isolated virus from 71 NLV-positive stool specimens from 37 outbreaks of nonbacterial acute gastroenteritis and seque...

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