Molecular diagnosis of African Swine Fever by a new real-time PCR using universal probe library.

J, Fernández-Pinero; C, Gallardo; M, Elizalde; A, Robles; C, Gómez; R, Bishop; L, Heath; E, Couacy-Hymann; FO, Fasina; V, Pelayo; A, Soler; M, Arias

doi:10.1111/j.1865-1682.2012.01317.x

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Molecular diagnosis of African Swine Fever by a new real-time PCR using universal probe library.

Shared by NobleBlocks on Mar 7, 2012 • 12:00 AM UTC

Authors:

Fernández-Pinero J

Gallardo C

Elizalde M

Abstract

A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the VP...

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