Sensitive, specific, and rapid detection of Leishmania donovani DNA by loop-mediated isothermal amplification.

H, Takagi; M, Itoh; MZ, Islam; A, Razzaque; AR, Ekram; Y, Hashighuchi; E, Noiri; E, Kimura

doi:10.4269/ajtmh.2009.09-0145

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Sensitive, specific, and rapid detection of Leishmania donovani DNA by loop-mediated isothermal amplification.

Shared by NobleBlocks on Oct 1, 2009 • 12:00 AM UTC

Authors:

Takagi H

Itoh M

Islam MZ

Abstract

We have applied a loop-mediated isothermal amplification (LAMP) technique to detect Leishmania donovani DNA. The LAMP technique detected 1 fg of L. donovani DNA, which was 10-fold more sensitive than a conventional polymerase chain reaction (PCR). All nested PCR-positive blood samples from visceral ...

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