Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.

M, Jinek; F, Jiang; DW, Taylor; SH, Sternberg; E, Kaya; E, Ma; C, Anders; M, Hauer; K, Zhou; S, Lin; M, Kaplan; AT, Iavarone; E, Charpentier; E, Nogales; JA, Doudna

doi:10.1126/science.1247997

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Structures of Cas9 endonucleases reveal RNA-mediated conformational activation.

Published in Science (New York, N.Y.) • Feb 6, 2014

Authors:

Jinek M

Jiang F

Taylor DW

Abstract

Type II CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems use an RNA-guided DNA endonuclease, Cas9, to generate double-strand breaks in invasive DNA during an adaptive bacterial immune response. Cas9 has been harnessed as a powerful tool for genome ed...

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