Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research.

M, Kitagawa; T, Ara; M, Arifuzzaman; T, Ioka-Nakamichi; E, Inamoto; H, Toyonaga; H, Mori

doi:10.1093/dnares/dsi012

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Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research.

Published in DNA research : an international journal for rapid publication of reports on genes and genomes • Jan 1, 2005

Authors:

Kitagawa M

Ara T

Arifuzzaman M

Abstract

Based on the genomic sequence data of Escherichia coli K-12 strain, we have constructed a complete set of cloned individual genes encoding Histidine-tagged proteins with or without GFP fused for functional genomic analysis. Each clone encodes a protein of predicted ORF attached by Histidines and sev...

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