Highly Multiplexed Subcellular RNA Sequencing in Situ

Hyuk Lee, Je; R. Daugharthy, Evan; Scheiman, Jonathan; Kalhor, Reza; L. Yang, Joyce; C. Ferrante, Thomas; Terry, Richard; S. F. Jeanty, Sauveur; Li, Chao; Amamoto, Ryoji; T. Peters, Derek; M. Turczyk, Brian; H. Marblestone, Adam; A. Inverso, Samuel; Bernard, Amy; Mali, Prashant; Rios, Xavier; Aach, John; M. Church, George

doi:10.1126/science.1250212

Public

Highly Multiplexed Subcellular RNA Sequencing in Situ

Shared by NobleBlocks on Mar 21, 2014 • 12:00 AM UTC

Authors:

Je Hyuk Lee

Evan R. Daugharthy

Jonathan Scheiman

Abstract

Transcripts Visualized in Situ Despite advances, current methods for single-cell sequencing are unable to resolve transcript location within the cell, so Lee et al. (p. 1360 , published online 27 February) developed a method of fluorescent in situ RNA sequencing (FISSEQ) that works in vivo to show m...

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