A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes

Janke, C.; Magiera, M.; Rathfelder, N.; Taxis, Christof; Reber, Simone; Maekawa, H.; Moreno-Borchart, A.; Doenges, G.; Schwob, E.; Schiebel, E.; Knop, M.

doi:10.1002/yea.1142

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A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes

Shared by NobleBlocks on Aug 1, 2004 • 12:00 AM UTC

Authors:

Carsten Janke

Maria M. Magiera

Nicole Rathfelder

Abstract

Abstract Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo in the yeast Saccharomyces cerevisiae . This strategy directs the amplified tags to the desired chromosomal loci due to flanking homologous sequences provided by...

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