Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

Goedhart, J.; von Stetten, D.; Noirclerc-Savoye, M.; Lelimousin, M.; Joosen, L.; Hink, M.; van Weeren, Laura; Gadella, T.; Royant, A.

doi:10.1038/ncomms1738

Public

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

Published in Nature Communications • Jan 1, 2012

Authors:

J. Goedhart

D. von Stetten

M. Noirclerc-Savoye

Abstract

Cyan variants of green fluorescent protein are widely used as donors in Förster resonance energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent Protein (ECFP) was sequentially improved into the brighter variants Super Cyan Fluorescent Protein 3A (SCFP3A) and mTurqu...

Subject

Medicine

Mathematics

Biology

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