Integrated Genomic and Proteomic Analyses of a Systematically Perturbed Metabolic Network

Ideker, Trey; Thorsson, Vesteinn; A. Ranish, Jeffrey; Christmas, Rowan; Buhler, Jeremy; K. Eng, Jimmy; Bumgarner, Roger; R. Goodlett, David; Aebersold, Ruedi; Hood, Leroy

doi:10.1126/science.292.5518.929

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Integrated Genomic and Proteomic Analyses of a Systematically Perturbed Metabolic Network

Shared by NobleBlocks on May 4, 2001 • 12:00 AM UTC

Authors:

Trey Ideker

Vesteinn Thorsson

Jeffrey A. Ranish

Abstract

We demonstrate an integrated approach to build, test, and refine a model of a cellular pathway, in which perturbations to critical pathway components are analyzed using DNA microarrays, quantitative proteomics, and databases of known physical interactions. Using this approach, we identify 997 messen...

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