Keto-polymethines: a versatile class of dyes with outstanding spectroscopic properties for <i>in cellulo</i> and <i>in vivo</i> two-photon microscopy imaging.

The synthesis of keto-heptamethine derivatives has been expanded to various new symmetrical and asymmetrical structures, including an unprecedented di-anionic keto-polymethine. The spectroscopic behavior of these new dyes has been systematically and thoroughly investigated, revealing that the format... View More