Expression of recombinant enhanced green fluorescent protein provides insight into foreign gene-expression differences between Mut+ and MutS strains of Pichia pastoris.

CW, Theron; J, Berrios; S, Steels; S, Telek; R, Lecler; C, Rodriguez; P, Fickers

doi:10.1002/yea.3388

Public

Expression of recombinant enhanced green fluorescent protein provides insight into foreign gene-expression differences between Mut+ and MutS strains of Pichia pastoris.

Shared by NobleBlocks on May 1, 2019 • 12:00 AM UTC

Authors:

Theron CW

Berrios J

Steels S

Abstract

Pichia pastoris is a very popular yeast for recombinant protein production, mainly due to the strong, methanol-inducible P<sub>AOX1</sub> promoter. Methanol induction however poses several drawbacks. One approach to improve processes is to use MutS strains with reduced methanol catabolic ability. Va...

Subject

Pichia pastoris

EGFP

Muts

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