Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer

MF, Scallan; C, Dempsey; J, MacSharry; I, O’Callaghan; PM, O’Connor; CP, Horgan; E, Durack; PD, Cotter; S, Hudson; HA, Moynihan; B, Lucey

doi:10.1101/2020.04.05.026435

Public

Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer

Shared by NobleBlocks on Apr 8, 2020 • 12:00 AM UTC

Authors:

Scallan MF

Dempsey C

MacSharry J

Abstract

The COVID-19 pandemic has resulted in increased need for diagnostic testing using reverse transcriptase real-time PCR (RT-PCR). An exponential increase in demand has resulted in a shortage of numerous reagents in particular those associated with the lysis buffer required to extract the viral RNA. He...

Discussions