Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Aggarwal, Abhi; Liu, Rui; Chen, Yang; J. Ralowicz, Amelia; J. Bergerson, Samuel; Tomaska, Filip; Mohar, Boaz; L. Hanson, Timothy; P. Hasseman, Jeremy; Reep, Daniel; Tsegaye, Getahun; Yao, Pantong; Ji, Xiang; Kloos, Marinus; Walpita, Deepika; Patel, Ronak; A. Mohr, Manuel; W. Tillberg, Paul; GENIE Project Team, The; L. Looger, Loren; S. Marvin, Jonathan; B. Hoppa, Michael; Konnerth, Arthur; Kleinfeld, David; R. Schreiter, Eric; Podgorski, Kaspar

doi:10.1038/s41592-023-01863-6

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Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Shared by NobleBlocks on May 4, 2023 • 12:00 AM UTC

Authors:

Abhi Aggarwal

Rui Liu

Yang Chen

Abstract

Abstract The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit low in vivo signal-to-noise ratios, saturating activation kinetics and exclusion from postsynaptic densities. Using a multias...

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